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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	paaG
Gene Synonym(s)	ECK1391, b1394, JW1389, ydbT^[1], ydbT
Product Desc.	putative enoyl-CoA hydratase/isomerase of phenylacetate degradation^[2]^[3] Phenylacetic acid degradation; mutants are unable to use phenylacetate as a carbon source^[4]
Product Synonyms(s)	acyl-CoA hydratase^[1], B1394^[2]^[1], YdbT^[2]^[1], PaaG^[2]^[1] , ECK1391, JW1389, ydbT, b1394
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): paaABCDEFGHIJK^[2]
Regulation/Activity
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Notes

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paaG mutants accumulate ring-1,2-dihydroxy-1,2-dihydrophenylacetyl lactone.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	paaG
Mnemonic	Phenylacetic acid
Synonyms	ECK1391, b1394, JW1389, ydbT^[1], ydbT
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	31.39 minutes	MG1655: 1456288..1457076 <gbrowseImage> name=NC_000913:1456288..1457076 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1348347..1349135 <gbrowseImage> name=NC_012759:1348347..1349135 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1459978..1460766 <gbrowseImage> name=NC_007779:1459978..1460766 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1546883..1547671 <gbrowseImage> name=NC_010473:1546883..1547671 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔpaaG (Keio:JW1389)	deletion	deletion		PMID:16738554	Shigen CGSC9228^[5]
paaGG121C		G121C	(in strain: W)			Strain variation; seeded from UniProt:P77467
ΔpaaG758::kan				PMID:16738554	CGSC:102226
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1389	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGGAATTCATCCTCAGTCA Primer 2:CCTTTCCCCGTGAACTGCGGTGA
5F1	Kohara Phage	Genobase	PMID:3038334
1A7	Kohara Phage	Genobase	PMID:3038334
zhj-3076::Tn10	Linked marker	CAG18640 = CGSC7453^[5]	est. P1 cotransduction: 43% ^[6]
trg-2::Tn10	Linked marker	CAG12026 = CGSC7376^[5]	est. P1 cotransduction: 21% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6715	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13741	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003309	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946263	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3504	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004661	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PaaG
Synonyms	acyl-CoA hydratase^[1], B1394^[2]^[1], YdbT^[2]^[1], PaaG^[2]^[1] , ECK1391, JW1389, ydbT, b1394
Product description	putative enoyl-CoA hydratase/isomerase of phenylacetate degradation^[2]^[3] Phenylacetic acid degradation; mutants are unable to use phenylacetate as a carbon source^[4]
EC number (for enzymes)	4.2.1.17^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001753	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004300	enoyl-CoA hydratase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1.17	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006629	lipid metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0443	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006631	fatty acid metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0276	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001753	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	sdhA		PMID:16606699	Experiment(s):EBI-1139758
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMEFILSHVE KGVMTLTLNR PERLNSFNDE MHAQLAECLK QVERDDTIRC LLLTGAGRGF CAGQDLNDRN VDPTGPAPDL GMSVERFYNP LVRRLAKLPK PVICAVNGVA AGAGATLALG GDIVIAARSA KFVMAFSKLG LIPDCGGTWL LPRVAGRARA MGLALLGNQL SAEQAHEWGM IWQVVDDETL ADTAQQLARH LATQPTFGLG LIKQAINSAE TNTLDTQLDL ERDYQRLAGR SADYREGVSA FLAKRSPQFT GK
Length	262
Mol. Wt	28.404 kDa
pI	6.9 (calculated)
Extinction coefficient	20,970 - 21,595 (calc based on 3 Y, 3 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	14..186	PF00378 Enoyl-CoA hydratase/isomerase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=paaG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129355	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946263	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004661	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77467	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6715	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13741	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946263	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003309	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3504	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
paaABCDEFGHIJK
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1456268..1456308 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1394 (EcoliWiki Page)	NCBI GEO profiles for paaG
microarray	GenExpDB:b1394 (EcoliWiki Page)	Summary of data for paaG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to paaG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6715	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3504	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1394
EcoGene	EcoGene:EG13741	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003309	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004661	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT4G16210 (score: 1.000; bootstrap: 64%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDR036C (score: 1.000; bootstrap: 85%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC2D109 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00025321001 (score: 1.000; bootstrap: 74%) GSTENP00017633001 (score: 0.114)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000040392 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00378 Enoyl-CoA hydratase/isomerase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:52096
EcoCyc	EcoCyc:G6715	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13741	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003309	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3504	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004661	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

paaG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to paaG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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