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Product(s) |

Expression|

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Standard Name	opgH
Gene Synonym(s)	ECK1035, b1049, JW1037, mdoA, mdoH^[1], mdoA, mdoH
Product Desc.	membrane glycosyltransferase; synthesis of membrane-derived oligosaccharide (MDO)^[2]^[3]
Product Synonyms(s)	glucan biosynthesis: glycosyl transferase^[1], MdoA^[2]^[1], B1049^[2]^[1], MdoH^[2]^[1] , ECK1035, JW1037, mdoA, mdoH, b1049
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mdoGH^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	opgH
Mnemonic	Osmoregulated periplasmic glucans
Synonyms	ECK1035, b1049, JW1037, mdoA, mdoH^[1], mdoA, mdoH
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	23.93 minutes	MG1655: 1110086..1112629 <gbrowseImage> name=NC_000913:1110086..1112629 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1125432..1127975 <gbrowseImage> name=NC_012967:1125432..1127975 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1014011..1016554 <gbrowseImage> name=NC_012759:1014011..1016554 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1112440..1114983 <gbrowseImage> name=NC_007779:1112440..1114983 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1165531..1168074 <gbrowseImage> name=NC_010473:1165531..1168074 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
Strain DH10B		P289L	Nonsynonomous mutation	PMID:18245285	ASAP	This is a SNP in E. coli K-12 Strain DH10B
ΔmdoH (Keio:JW1037)	deletion	deletion		PMID:16738554	Shigen CGSC9001^[4]
mdoH::Tn5KAN-2 (FB21169)	Insertion at nt 741 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20092	does not contain pKD46
ΔmdoH720::kan				PMID:16738554	CGSC:101784
Strain BW2952	CC865-866TT			PMID:19376874		Relative to MG1655.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1037	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAAGACAACTGAGTACAT Primer 2:CCTTGCGAAGCCGCATCCGGTTT
1H7	Kohara Phage	Genobase	PMID:3038334
E4H10S	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[4]	est. P1 cotransduction: 12% ^[5]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[4]	est. P1 cotransduction: 13% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11886	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1832	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1049
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C1314
EcoGene	EcoGene:EG11886	Escherichia coli str. K-12 substr. MG1655
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:MDOH
CGSC (EcoliWiki Page)	CGSC:31852
ASAP	ASAP:ABE-0003560	Escherichia coli str. K-12 substr. MG1655
EcoCyc (EcoliWiki Page)	ECOCYC:EG11886	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945624	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001816	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MdoH
Synonyms	glucan biosynthesis: glycosyl transferase^[1], MdoA^[2]^[1], B1049^[2]^[1], MdoH^[2]^[1] , ECK1035, JW1037, mdoA, mdoH, b1049
Product description	membrane glycosyltransferase; synthesis of membrane-derived oligosaccharide (MDO)^[2]^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01072	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009250	glucan biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01072	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016757	transferase activity, transferring glycosyl groups	PMID:1320618	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0016757	transferase activity, transferring glycosyl groups	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0328	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009250	glucan biosynthetic process	PMID:9352941	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0016758	transferase activity, transferring hexosyl groups	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01072	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006970	response to osmotic stress	PMID:9352941	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0005887	integral to plasma membrane	PMID:9352918	IDA: Inferred from Direct Assay		C		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence
plasma membrane	C-terminus localized in the cytoplasm with 6 predicted transmembrane domains	Daley et al. (2005) ^[6]
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNKTTEYIDA MPIAASEKAA LPKTDIRAVH QALDAEHRTW AREDDSPQGS VKARLEQAWP DSLADGQLIK DDEGRDQLKA MPEAKRSSMF PDPWRTNPVG RFWDRLRGRD VTPRYLARLT KEEQESEQKW RTVGTIRRYI LLILTLAQTV VATWYMKTIL PYQGWALINP MDMVGQDLWV SFMQLLPYML QTGILILFAV LFCWVSAGFW TALMGFLQLL IGRDKYSISA STVGDEPLNP EHRTALIMPI CNEDVNRVFA GLRATWESVK ATGNAKHFDV YILSDSYNPD ICVAEQKAWM ELIAEVGGEG QIFYRRRRRR VKRKSGNIDD FCRRWGSQYS YMVVLDADSV MTGDCLCGLV RLMEANPNAG IIQSSPKASG MDTLYARCQQ FATRVYGPLF TAGLHFWQLG ESHYWGHNAI IRVKPFIEHC ALAPLPGEGS FAGSILSHDF VEAALMRRAG WGVWIAYDLP GSYEELPPNL LDELKRDRRW CHGNLMNFRL FLVKGMHPVH RAVFLTGVMS YLSAPLWFMF LALSTALQVV HALTEPQYFL QPRQLFPVWP QWRPELAIAL FASTMVLLFL PKLLSILLIW CKGTKEYGGF WRVTLSLLLE VLFSVLLAPV RMLFHTVFVV SAFLGWEVVW NSPQRDDDST SWGEAFKRHG SQLLLGLVWA VGMAWLDLRF LFWLAPIVFS LILSPFVSVI SSRATVGLRT KRWKLFLIPE EYSPPQVLVD TDRFLEMNRQ RSLDDGFMHA VFNPSFNALA TAMATARHRA SKVLEIARDR HVEQALNETP EKLNRDRRLV LLSDPVTMAR LHFRVWNSPE RYSSWVSYYE GIKLNPLALR KPDAASQ
Length	847
Mol. Wt	96.939 kDa
pI	8.9 (calculated)
Extinction coefficient	211,760 - 213,010 (calc based on 24 Y, 32 W, and 10 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	245..430	PF00535 Glycosyl transferase family 2		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mdoH taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129012	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945624	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003560	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P62517	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11886	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11886	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945624	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001816	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1832	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	30.192+/-0.22	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.042272906	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1234	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	304	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	519	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mdoGH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1110066..1110106 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1049 (EcoliWiki Page)	NCBI GEO profiles for mdoH
microarray	GenExpDB:b1049 (EcoliWiki Page)	Summary of data for mdoH from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to opgH Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11886	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1832	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1049
EcoGene	EcoGene:EG11886	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001816	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003560	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MDOH	From SHIGELLACYC
E. coli O157	MDOH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00535 Glycosyl transferase family 2
Superfamily (EcoliWiki Page)	SUPERFAMILY:53448
EcoCyc	EcoCyc:EG11886	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11886	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001816	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1832	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003560	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

opgH:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to opgH Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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