PMID:20081027
| Citation |
Arutyunov, D, Arenson, B, Manchak, J and Frost, LS (2010) F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation. J. Bacteriol. 192:1730-4 |
|---|---|
| Abstract |
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH. |
| Links |
PubMed PMC2832511 Online version:10.1128/JB.00726-09 |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Cell Membrane/chemistry; Conjugation, Genetic/physiology; Conserved Sequence; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; F Factor; Molecular Sequence Data; Mutation; Nuclear Proteins/chemistry; Nuclear Proteins/metabolism; Protein Structure, Tertiary; Protein Transport |
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