PMID:20081027
Citation |
Arutyunov, D, Arenson, B, Manchak, J and Frost, LS (2010) F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation. J. Bacteriol. 192:1730-4 |
---|---|
Abstract |
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH. |
Links |
PubMed PMC2832511 Online version:10.1128/JB.00726-09 |
Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Cell Membrane/chemistry; Conjugation, Genetic/physiology; Conserved Sequence; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; F Factor; Molecular Sequence Data; Mutation; Nuclear Proteins/chemistry; Nuclear Proteins/metabolism; Protein Structure, Tertiary; Protein Transport |
edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.