PMID:11804582

From EcoliWiki
Jump to: navigation, search
Citation

Zhang, A, Wassarman, KM, Ortega, J, Steven, AC and Storz, G (2002) The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs. Mol. Cell 9:11-22

Abstract

The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhlA and rpoS mRNA translation. Here we report that Hfq is a bacterial homolog of the Sm and Sm-like proteins integral to RNA processing and mRNA degradation complexes in eukaryotic cells. Hfq exhibits the hallmark features of Sm and Sm-like proteins: the Sm1 sequence motif, a multisubunit ring structure (in this case a homomeric hexamer), and preferential binding to polyU. We also show that Hfq increases the OxyS RNA interaction with its target messages and propose that the enhancement of RNA-RNA pairing may be a general function of Hfq, Sm, and Sm-like proteins.

Links

PubMed

Keywords

Amino Acid Sequence; Autoantigens/genetics; Autoantigens/metabolism; Bacterial Proteins/genetics; Carrier Proteins/genetics; Carrier Proteins/metabolism; Escherichia coli; Escherichia coli Proteins; Host Factor 1 Protein; Humans; Integration Host Factors; Molecular Sequence Data; Protein Binding; Protein Biosynthesis; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/metabolism; Ribonucleoproteins, Small Nuclear/genetics; Ribonucleoproteins, Small Nuclear/metabolism; Sequence Alignment; Sigma Factor/genetics; Trans-Activators/genetics; Transcription Factors/genetics; Transcription Factors/metabolism; snRNP Core Proteins

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.