napA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
napA |
---|---|
Gene Synonym(s) |
ECK2198, b2206, JW2194, yojC, yojD, yojE[1], yojE |
Product Desc. |
large subunit of periplasmic nitrate reductase, molybdoprotein[2][3]; Component of molybdoprotein complex[2][3]; periplasmic nitrate reductase[2][3]; periplasmic nitrate reductase[3] Nitrate reductase, periplasmic[4] |
Product Synonyms(s) |
nitrate reductase, periplasmic, large subunit[1], B2206[2][1], YojE[2][1], YojC[2][1], YojD[2][1], NapA[2][1] , ECK2198, JW2194, yojC, yojD, yojE, b2206 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression |
transcription unit(s): napFDAGHBC[2], napFDAGHBC-ccmABCDEFGH[2], aeg-46.5 |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Verified Tat substrate: NapA has a Tat-only (Class I) 36 aa signal peptide (Tullman-Ercek, 2007).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
napA |
---|---|
Mnemonic |
NitrAte reductase, Periplasmic |
Synonyms |
ECK2198, b2206, JW2194, yojC, yojD, yojE[1], yojE |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
49.54 minutes |
MG1655: 2300775..2298289 |
||
NC_012967: 2252742..2250256 |
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NC_012759: 2184094..2186580 |
||||
W3110 |
|
W3110: 2306087..2303601 |
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DH10B: 2391763..2389277 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
2298292 |
Edman degradation |
PMID:10234835 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔnapA (Keio:JW2194) |
deletion |
deletion |
PMID:16738554 |
||||
napA722::Tn10 |
Insertion at 2,299,612 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[6] |
Synonyms: zeg-722, zei-722::Tn10 | ||||
napA722::Tn10 |
PMID:2540407 PMID:9829956 |
||||||
napA3130::Tn10kan |
PMID:2540407 PMID:9829956 |
||||||
ΔnapA759::kan |
PMID:16738554 |
| |||||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW2194 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAAACTCAGTCGTCGTAGCTT Primer 2:CCaACCTTCTCCAGTTTGACCGC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: % [6] | ||
Linked marker |
est. P1 cotransduction: 45% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001976 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB1994 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007289 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
NapA |
---|---|
Synonyms |
nitrate reductase, periplasmic, large subunit[1], B2206[2][1], YojE[2][1], YojC[2][1], YojD[2][1], NapA[2][1] , ECK2198, JW2194, yojC, yojD, yojE, b2206 |
Product description |
large subunit of periplasmic nitrate reductase, molybdoprotein[2][3]; Component of molybdoprotein complex[2][3]; periplasmic nitrate reductase[2][3]; periplasmic nitrate reductase[3] Nitrate reductase, periplasmic[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0030288 |
outer membrane-bounded periplasmic space |
C |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
GO:0009061 |
anaerobic respiration |
PMID:11844760 |
IGI: Inferred from Genetic Interaction |
EcoliWiki:narG |
P |
complete | ||
GO:0005488 |
binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009010 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005506 |
iron ion binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01630 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005506 |
iron ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0408 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006777 |
Mo-molybdopterin cofactor biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01630 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0813 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008940 |
nitrate reductase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01630 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008940 |
nitrate reductase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010051 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008940 |
nitrate reductase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:1.7.99.4 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008940 |
nitrate reductase activity |
PMID:11844760 |
IGI: Inferred from Genetic Interaction |
F |
Seeded from EcoCyc (v14.0) |
Missing: with/from | ||
GO:0009055 |
electron carrier activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01630 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009055 |
electron carrier activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006655 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009061 |
anaerobic respiration |
PMID:11844760 |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | ||
GO:0022900 |
electron transport chain |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0249 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0030151 |
molybdenum ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006657 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008940 |
nitrate reductase activity |
PMID:11844760 |
IGI: Inferred from Genetic Interaction |
EcoliWiki:narG |
F |
complete | ||
GO:0030151 |
molybdenum ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010051 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0030151 |
molybdenum ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0500 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042128 |
nitrate assimilation |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01630 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042128 |
nitrate assimilation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010051 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042128 |
nitrate assimilation |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0534 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042597 |
periplasmic space |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010051 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042597 |
periplasmic space |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0574 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0042597 |
periplasmic space |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0200 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0046872 |
metal ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0479 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051536 |
iron-sulfur cluster binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0411 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010051 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0004 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01630 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010051 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0055114 |
oxidation reduction |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0560 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of molybdoprotein complex |
could be indirect |
||
Protein |
aceE |
PMID:15690043 |
Experiment(s):EBI-886395 | |
Protein |
lpdA |
PMID:15690043 |
Experiment(s):EBI-886395 | |
Protein |
panC |
PMID:15690043 |
Experiment(s):EBI-892863 | |
Protein |
rpmB |
PMID:15690043 |
Experiment(s):EBI-892863 | |
Protein |
rpsN |
PMID:15690043 |
Experiment(s):EBI-892863 | |
Protein |
ushA |
PMID:15690043 |
Experiment(s):EBI-892863 | |
Protein |
yacL |
PMID:15690043 |
Experiment(s):EBI-892863 | |
Protein |
panC |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpmB |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpsN |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
yacL |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
nusG |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
ushA |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
Subunits of periplasmic nitrate reductase |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
Periplasm |
This protein is exported via the Tat-system and has a long signal peptide with an usual cleavage site. |
PubMedID:10234835 |
||
Periplasm |
PMID:10234835 |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MKLSRRSFMK ANAVAAAAAA AGLSVPGVAR AVVGQQEAIK WDKAPCRFCG TGCGVLVGTQ QGRVVACQGD PDAPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKNGKYD KEGEFTPITW DQAFDVMEEK FKTALKEKGP ESIGMFGSGQ WTIWEGYAAS KLFKAGFRSN NIDPNARHCM ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGANMAEMH PILWSRITNR RLSNQNVTVA VLSTYQHRSF ELADNGIIFT PQSDLVILNY IANYIIQNNA INQDFFSKHV NLRKGATDIG YGLRPTHPLE KAAKNPGSDA SEPMSFEDYK AFVAEYTLEK TAEMTGVPKD QLEQLAQLYA DPNKKVISYW TMGFNQHTRG VWANNLVYNL HLLTGKISQP GCGPFSLTGQ PSACGTAREV GTFAHRLPAD MVVTNEKHRD ICEKKWNIPS GTIPAKIGLH AVAQDRALKD GKLNVYWTMC TNNMQAGPNI NEERMPGWRD PRNFIIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWRQQVQ APGEAKSDLW QLVQFSRRFK TEEVWPEDLL AKKPELRGKT LYEVLYATPE VSKFPVSELA EDQLNDESRE LGFYLQKGLF EEYAWFGRGH GHDLAPFDDY HKARGLRWPV VNGKETQWRY SEGNDPYVKA GEGYKFYGKP DGKAVIFALP FEPAAEAPDE EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARDLRR GDKVKVVSRR GEVISIVETR GRNRPPQGLV YMPFFDAAQL VNKLTLDATD PLSKETDFKK CAVKLEKV |
Length |
828 |
Mol. Wt |
93.042 kDa |
pI |
8.2 (calculated) |
Extinction coefficient |
151,720 - 153,220 (calc based on 28 Y, 20 W, and 12 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0007289 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120001976 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1994 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MG1655 |
165 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
57 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
30 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
![]() Figure courtesy of RegulonDB |
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2300755..2300795
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for napA | |
microarray |
Summary of data for napA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to napA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1994 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001976 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007289 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
NAPA |
From SHIGELLACYC |
E. coli O157 |
NAPA |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12067 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001976 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1994 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0007289 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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