napA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

napA

Gene Synonym(s)

ECK2198, b2206, JW2194, yojC, yojD, yojE[1], yojE

Product Desc.

large subunit of periplasmic nitrate reductase, molybdoprotein[2][3];

Component of molybdoprotein complex[2][3]; periplasmic nitrate reductase[2][3]; periplasmic nitrate reductase[3]

Nitrate reductase, periplasmic[4]

Product Synonyms(s)

nitrate reductase, periplasmic, large subunit[1], B2206[2][1], YojE[2][1], YojC[2][1], YojD[2][1], NapA[2][1] , ECK2198, JW2194, yojC, yojD, yojE, b2206

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): napFDAGHBC[2], napFDAGHBC-ccmABCDEFGH[2], aeg-46.5

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Verified Tat substrate: NapA has a Tat-only (Class I) 36 aa signal peptide (Tullman-Ercek, 2007).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

napA

Mnemonic

NitrAte reductase, Periplasmic

Synonyms

ECK2198, b2206, JW2194, yojC, yojD, yojE[1], yojE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

49.54 minutes 

MG1655: 2300775..2298289
<gbrowseImage> name=NC_000913:2298289..2300775 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2252742..2250256
<gbrowseImage> name=NC_012967:2250256..2252742 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2184094..2186580
<gbrowseImage> name=NC_012759:2184094..2186580 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2306087..2303601
<gbrowseImage> name=NC_007779:2303601..2306087 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2391763..2389277
<gbrowseImage> name=NC_010473:2389277..2391763 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2298292

Edman degradation

PMID:10234835


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔnapA (Keio:JW2194)

deletion

deletion

PMID:16738554

Shigen
CGSC9774[5]

napA722::Tn10

Insertion at 2,299,612 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG12098 = CGSC7400[5]

Synonyms: zeg-722, zei-722::Tn10

napA722::Tn10

PMID:2540407 PMID:9829956

CGSC:40001

napA3130::Tn10kan

PMID:2540407 PMID:9829956

CGSC:40004

ΔnapA759::kan

PMID:16738554

CGSC:102010


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2194

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAACTCAGTCGTCGTAGCTT

Primer 2:CCaACCTTCTCCAGTTTGACCGC

19D1

Kohara Phage

Genobase

PMID:3038334

22A6

Kohara Phage

Genobase

PMID:3038334

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[5]

est. P1 cotransduction: % [6]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[5]

est. P1 cotransduction: 45% [6]
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12067

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12067

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001976

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947093

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1994

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007289

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NapA

Synonyms

nitrate reductase, periplasmic, large subunit[1], B2206[2][1], YojE[2][1], YojC[2][1], YojD[2][1], NapA[2][1] , ECK2198, JW2194, yojC, yojD, yojE, b2206

Product description

large subunit of periplasmic nitrate reductase, molybdoprotein[2][3];

Component of molybdoprotein complex[2][3]; periplasmic nitrate reductase[2][3]; periplasmic nitrate reductase[3]

Nitrate reductase, periplasmic[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0030288

outer membrane-bounded periplasmic space

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0009061

anaerobic respiration

PMID:11844760

IGI: Inferred from Genetic Interaction

EcoliWiki:narG

P

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009010

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01630

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0006777

Mo-molybdopterin cofactor biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01630

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01630

F

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010051

F

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.7.99.4

F

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

PMID:11844760

IGI: Inferred from Genetic Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0009055

electron carrier activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01630

F

Seeded from EcoCyc (v14.0)

complete

GO:0009055

electron carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006655

F

Seeded from EcoCyc (v14.0)

complete

GO:0009061

anaerobic respiration

PMID:11844760

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0022900

electron transport chain

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0249

P

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006657

F

Seeded from EcoCyc (v14.0)

complete

GO:0008940

nitrate reductase activity

PMID:11844760

IGI: Inferred from Genetic Interaction

EcoliWiki:narG

F

complete

GO:0030151

molybdenum ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010051

F

Seeded from EcoCyc (v14.0)

complete

GO:0030151

molybdenum ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0500

F

Seeded from EcoCyc (v14.0)

complete

GO:0042128

nitrate assimilation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01630

P

Seeded from EcoCyc (v14.0)

complete

GO:0042128

nitrate assimilation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010051

P

Seeded from EcoCyc (v14.0)

complete

GO:0042128

nitrate assimilation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0534

P

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010051

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0200

C

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051536

iron-sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0411

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010051

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01630

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010051

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of molybdoprotein complex

could be indirect

Protein

aceE

PMID:15690043

Experiment(s):EBI-886395

Protein

lpdA

PMID:15690043

Experiment(s):EBI-886395

Protein

panC

PMID:15690043

Experiment(s):EBI-892863

Protein

rpmB

PMID:15690043

Experiment(s):EBI-892863

Protein

rpsN

PMID:15690043

Experiment(s):EBI-892863

Protein

ushA

PMID:15690043

Experiment(s):EBI-892863

Protein

yacL

PMID:15690043

Experiment(s):EBI-892863

Protein

panC

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmB

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

yacL

PMID:19402753

LCMS(ID Probability):99.6

Protein

nusG

PMID:19402753

LCMS(ID Probability):99.6

Protein

ushA

PMID:19402753

LCMS(ID Probability):99.6

Protein

Subunits of periplasmic nitrate reductase

could be indirect


</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Periplasm

This protein is exported via the Tat-system and has a long signal peptide with an usual cleavage site.

PubMedID:10234835

Periplasm

PMID:10234835

EchoLocation:napA


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKLSRRSFMK ANAVAAAAAA AGLSVPGVAR AVVGQQEAIK WDKAPCRFCG TGCGVLVGTQ
QGRVVACQGD PDAPVNRGLN CIKGYFLPKI MYGKDRLTQP LLRMKNGKYD KEGEFTPITW
DQAFDVMEEK FKTALKEKGP ESIGMFGSGQ WTIWEGYAAS KLFKAGFRSN NIDPNARHCM
ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGANMAEMH PILWSRITNR RLSNQNVTVA
VLSTYQHRSF ELADNGIIFT PQSDLVILNY IANYIIQNNA INQDFFSKHV NLRKGATDIG
YGLRPTHPLE KAAKNPGSDA SEPMSFEDYK AFVAEYTLEK TAEMTGVPKD QLEQLAQLYA
DPNKKVISYW TMGFNQHTRG VWANNLVYNL HLLTGKISQP GCGPFSLTGQ PSACGTAREV
GTFAHRLPAD MVVTNEKHRD ICEKKWNIPS GTIPAKIGLH AVAQDRALKD GKLNVYWTMC
TNNMQAGPNI NEERMPGWRD PRNFIIVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER
RTQFWRQQVQ APGEAKSDLW QLVQFSRRFK TEEVWPEDLL AKKPELRGKT LYEVLYATPE
VSKFPVSELA EDQLNDESRE LGFYLQKGLF EEYAWFGRGH GHDLAPFDDY HKARGLRWPV
VNGKETQWRY SEGNDPYVKA GEGYKFYGKP DGKAVIFALP FEPAAEAPDE EYDLWLSTGR
VLEHWHTGSM TRRVPELHRA FPEAVLFIHP LDAKARDLRR GDKVKVVSRR GEVISIVETR
GRNRPPQGLV YMPFFDAAQL VNKLTLDATD PLSKETDFKK CAVKLEKV
Length

828

Mol. Wt

93.042 kDa

pI

8.2 (calculated)

Extinction coefficient

151,720 - 153,220 (calc based on 28 Y, 20 W, and 12 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-36

UniProt Manual:Signal Peptides

UniProt:P33937

Domain

39..93

PF04879 Molybdopterin oxidoreductase Fe4S4 domain

PMID:19920124

Domain

96..568

PF00384 Molybdopterin oxidoreductase

PMID:19920124

Domain

714..822

PF01568 Molydopterin dinucleotide binding domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=napA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

<protect>

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130143

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947093

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007289

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P33937

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12067

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12067

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947093

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001976

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1994

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

165

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

57

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

30

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

napFDAGHBC

napFDAGHBC-ccmABCDEFGH

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2300755..2300795 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2206 (EcoliWiki Page)

NCBI GEO profiles for napA

microarray

GenExpDB:b2206 (EcoliWiki Page)

Summary of data for napA from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to napA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12067

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1994

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2206

EcoGene

EcoGene:EG12067

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001976

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007289

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

NAPA

From SHIGELLACYC

E. coli O157

NAPA

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF04879 Molybdopterin oxidoreductase Fe4S4 domain

Pfam (EcoliWiki Page)

PF01568 Molydopterin dinucleotide binding domain

Pfam (EcoliWiki Page)

PF00384 Molybdopterin oxidoreductase

Superfamily (EcoliWiki Page)

SUPERFAMILY:50692

Panther (EcoliWiki Page)

PTHR11615:SF42

Superfamily (EcoliWiki Page)

SUPERFAMILY:53706

EcoCyc

EcoCyc:EG12067

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12067

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001976

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1994

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007289

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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