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Gene |

Product(s) |

Expression|

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Standard Name	nanM
Gene Synonym(s)	ECK4301, b4310, JW5777, yjhT^[1], JW5777, yjhT
Product Desc.	conserved protein^[2]^[3]
Product Synonyms(s)	conserved protein^[1], B4310^[2]^[1], YjhT^[2]^[1] , ECK4301, JW5777, yjhT, b4310
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nanM
Mnemonic	N-Acetylneuraminate
Synonyms	ECK4301, b4310, JW5777, yjhT^[1], JW5777, yjhT
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	97.76 minutes, 97.76 minutes	MG1655: 4536788..4535682 <gbrowseImage> name=NC_000913:4535682..4536788 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 4474417..4475523 <gbrowseImage> name=NC_012759:4474417..4475523 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4543445..4542339 <gbrowseImage> name=NC_007779:4542339..4543445 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
yjhT(del) (Keio:JW5777)	deletion	deletion		PMID:16738554	Shigen
nanMK30A		K30A	No effect on catalytic activity			seeded from UniProt:P39371
nanME228A		E228A	Great decrease in catalytic activity			seeded from UniProt:P39371
nanMR234A		R234A	Decrease in catalytic activity			seeded from UniProt:P39371
nanMH297A		H297A	No effect on catalytic activity			seeded from UniProt:P39371
nanMK302A		K302A	No effect on catalytic activity			seeded from UniProt:P39371
nanMY328A		Y328A	No effect on catalytic activity			seeded from UniProt:P39371
nanME344A		E344A	No effect on catalytic activity			seeded from UniProt:P39371
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5777	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAAAACAATAACGGCGCT Primer 2:CCGTTTTGTACTGTGACTTTATT
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[4]	est. P1 cotransduction: % ^[5] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[4]	est. P1 cotransduction: 5% ^[5] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7920	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12562	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004415	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949106	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2450	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014132	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YjhT
Synonyms	conserved protein^[1], B4310^[2]^[1], YjhT^[2]^[1] , ECK4301, JW5777, yjhT, b4310
Product description	conserved protein^[2]^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki. See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005975	carbohydrate metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0119	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016857	racemase and epimerase activity, acting on carbohydrates and derivatives	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01195	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016857	racemase and epimerase activity, acting on carbohydrates and derivatives	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.1.3	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpsT	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplP	PMID:19402753	LCMS(ID Probability):99.6
Protein	yejK	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplW	PMID:19402753	LCMS(ID Probability):99.6
Protein	dps	PMID:19402753	LCMS(ID Probability):99.6
Protein	pspC	PMID:19402753	LCMS(ID Probability):98.2
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See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki. </protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Periplasm	Soluble periplasmic protein. Normal Sec-dependent signal peptide	^[6]
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See Help:Product_localization for how to add or edit information in this section of EcoliWiki. <protect></protect>

Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTTLTVRVFT TAEIIYRKTV IALVCHLNCS RQETVTMNKT ITALAIMMAS FAANASVLPE TPVPFKSGTG AIDNDTVYIG LGSAGTAWYK LDTQAKDKKW TALAAFPGGP RDQATSAFID GNLYVFGGIG KNSEGLTQVF NDVHKYNPKT NSWVKLMSHA PMGMAGHVTF VHNGKAYVTG GVNQNIFNGY FEDLNEAGKD STAIDKINAH YFDKKAEDYF FNKFLLSFDP STQQWSYAGE SPWYGTAGAA VVNKGDKTWL INGEAKPGLR TDAVFELDFT GNNLKWNKLA PVSSPDGVAG GFAGISNDSL IFAGGAGFKG SRENYQNGKN YAHEGLKKSY STDIHLWHNG KWDKSGELSQ GRAYGVSLPW NNSLLIIGGE TAGGKAVTDS VLITVKDNKV TVQN
Length	404
Mol. Wt	43.667 kDa
pI	8.5 (calculated)
Extinction coefficient	77,350 - 77,600 (calc based on 15 Y, 10 W, and 2 C residues)
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See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	74..122	PF01344 Kelch motif		PMID:19920124
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nanM taxon=562,83333 </beststructure>

Models

View models at:

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See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111727	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949106	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014132	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39371	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7920	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12562	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949106	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004415	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2450	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	1.673+/-0.343	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.011754069	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	53	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	128	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	29^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yjhT
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4536768..4536808 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4310 (EcoliWiki Page)	NCBI GEO profiles for yjhT
microarray	GenExpDB:b4310 (EcoliWiki Page)	Summary of data for yjhT from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nanM Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7920	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2450	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4310
EcoGene	EcoGene:EG12562	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004415	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014132	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Ciona intestinalis	ENSCINP00000005165 (score: 1.000; bootstrap: 100%) ENSCINP00000017827 (score: 0.382) ENSCINP00000013854 (score: 0.378) ENSCINP00000005691 (score: 0.342) ENSCINP00000013720 (score: 0.168) ENSCINP00000013317 (score: 0.166) ENSCINP00000002479 (score: 0.162) ENSCINP00000008135 (score: 0.147) ENSCINP00000005810 (score: 0.140) ENSCINP00000009330 (score: 0.135) ENSCINP00000014080 (score: 0.123) ENSCINP00000017048 (score: 0.122) ENSCINP00000006415 (score: 0.117) ENSCINP00000014893 (score: 0.115) ENSCINP00000002505 (score: 0.115) ENSCINP00000006099 (score: 0.106) ENSCINP00000011706 (score: 0.101) ENSCINP00000003436 (score: 0.079) ENSCINP00000020667 (score: 0.079) ENSCINP00000003285 (score: 0.074) ENSCINP00000004954 (score: 0.069) ENSCINP00000019558 (score: 0.056) ENSCINP00000012688 (score: 0.056) ENSCINP00000014153 (score: 0.055) ENSCINP00000000883 (score: 0.053)	From Inparanoid:20070104
Shigella flexneri	YJHT	From SHIGELLACYC
E. coli O157	YJHT	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01344 Kelch motif
Panther (EcoliWiki Page)	PTHR23244:SF132
Superfamily (EcoliWiki Page)	SUPERFAMILY:117281
EcoCyc	EcoCyc:G7920	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12562	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004415	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2450	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014132	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Severi, E et al. (2008) Sialic acid mutarotation is catalyzed by the Escherichia coli beta-propeller protein YjhT. J. Biol. Chem. 283 4841-9 PubMed

nanM:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nanM Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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