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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	nanE
Gene Synonym(s)	ECK3212, b3223, JW3192, yhcJ^[1], yhcJ
Product Desc.	NANE^[2]^[3] ManNAc-6-P to GlcNAc-6-P epimerase^[4]
Product Synonyms(s)	predicted N-acetylmannosamine-6-P epimerase^[1], B3223^[2]^[1], YhcJ^[2]^[1], NanE^[2]^[1] , ECK3212, JW3192, yhcJ, b3223
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nanATEK-yhcH^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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NanE is not predicted to be a lipoprotein by LipOP or PATOSEQ, but is listed in DOLOP as a putative lipoprotein. Although the Cys is conserved, none of the homologs are predicted by LipoP to be a lipoprotein, so it is likely not to be a lipoprotein. NanE was just below the PATOSEQ cutoff for a lipoprotein prediction.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nanE
Mnemonic	N-Acetylneuraminate
Synonyms	ECK3212, b3223, JW3192, yhcJ^[1], yhcJ
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	72.6 minutes	MG1655: 3369058..3368369 <gbrowseImage> name=NC_000913:3368369..3369058 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3298934..3298245 <gbrowseImage> name=NC_012967:3298245..3298934 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3255517..3256206 <gbrowseImage> name=NC_012759:3255517..3256206 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3370891..3370202 <gbrowseImage> name=NC_007779:3370202..3370891 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3466803..3466114 <gbrowseImage> name=NC_010473:3466114..3466803 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔnanE (Keio:JW3192)	deletion	deletion	PMID:16738554	Shigen CGSC10421^[5]
yhcJ::Tn5KAN-I-SceI (FB21070)	Insertion at nt 385 in Plus orientation		PMID:15262929	E. coli Genome Project:FB21070	contains pKD46
ΔnanE751::kan			PMID:16738554	CGSC:102002
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3192	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCGTTACTTGCACAACTGGA Primer 2:CCTAGCACCGCCTTTTTCATCGC
8D6	Kohara Phage	Genobase	PMID:3038334
7C8	Kohara Phage	Genobase	PMID:3038334
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[5]	est. P1 cotransduction: 86% ^[6] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[5]	est. P1 cotransduction: 12% ^[6] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7677	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12816	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004224	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947745	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2667	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010575	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NanE
Synonyms	predicted N-acetylmannosamine-6-P epimerase^[1], B3223^[2]^[1], YhcJ^[2]^[1], NanE^[2]^[1] , ECK3212, JW3192, yhcJ, b3223
Product description	NANE^[2]^[3] ManNAc-6-P to GlcNAc-6-P epimerase^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011060	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016853	isomerase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0413	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016857	racemase and epimerase activity, acting on carbohydrates and derivatives	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01235	F	Seeded from EcoCyc (v14.0)	complete
	GO:0047465	N-acylglucosamine-6-phosphate 2-epimerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007260	F	Seeded from EcoCyc (v14.0)	complete
	GO:0047465	N-acylglucosamine-6-phosphate 2-epimerase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.1.3.9	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dcyD	PMID:15690043	Experiment(s):EBI-882084
Protein	ttdR	PMID:16606699	Experiment(s):EBI-1145183
Protein	aldB	PMID:16606699	Experiment(s):EBI-1145183
Protein	dcyD	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSLLAQLDQK IAANGGLIVS CQPVPDSPLD KPEIVAAMAL AAEQAGAVAI RIEGVANLQA TRAVVSVPII GIVKRDLEDS PVRITAYIED VDALAQAGAD IIAIDGTDRP RPVPVETLLA RIHHHGLLAM TDCSTPEDGL ACQKLGAEII GTTLSGYTTP ETPEEPDLAL VKTLSDAGCR VIAEGRYNTP AQAADAMRHG AWAVTVGSAI TRLEHICQWY NTAMKKAVL
Length	229
Mol. Wt	24.073 kDa
pI	4.8 (calculated)
Extinction coefficient	16,960 - 17,585 (calc based on 4 Y, 2 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	35..226	PF04131 Putative N-acetylmannosamine-6-phosphate epimerase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nanE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131113	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947745	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010575	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A761	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7677	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12816	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947745	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004224	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2667	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.29E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 MG1655	93	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	39	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	61^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nanATEK-yhcH
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3369038..3369078 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3223 (EcoliWiki Page)	NCBI GEO profiles for nanE
microarray	GenExpDB:b3223 (EcoliWiki Page)	Summary of data for nanE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nanE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7677	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2667	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3223
EcoGene	EcoGene:EG12816	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004224	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010575	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000020525 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YHCJ	From SHIGELLACYC
E. coli O157	YHCJ	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04131 Putative N-acetylmannosamine-6-phosphate epimerase
Superfamily (EcoliWiki Page)	SUPERFAMILY:51366
EcoCyc	EcoCyc:G7677	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12816	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004224	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2667	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010575	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

nanE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nanE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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