nagE:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
nagE |
---|---|
Gene Synonym(s) |
ECK0667, b0679, JW0665, pstN[1], pstN, ptsN |
Product Desc. |
Component of N-acetylglucosamine PTS permease[2][3] N-acetylglucosamine-specific enzyme II of phosphotransferase system[4] |
Product Synonyms(s) |
fused N-acetyl glucosamine specific PTS enzymes: IIC component[1], IIB component[1], IIA component[1], B0679[2][1], PstN[2][1], NagE[2][1] , ECK0667, JW0665, pstN, ptsN, b0679 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
nagE |
---|---|
Mnemonic |
N-Acetylglucosamine |
Synonyms |
ECK0667, b0679, JW0665, pstN[1], pstN, ptsN |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
15.16 minutes |
MG1655: 703167..705113 |
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NC_012967: 686039..687985 |
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NC_012759: 605927..607873 |
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W3110 |
|
W3110: 704366..706312 |
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DH10B: 755759..757705 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
nagE(del) (Keio:JW0665) |
deletion |
deletion |
PMID:16738554 |
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nagE467 |
|||||||
nagE728(del)::kan |
PMID:16738554 |
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nagE |
Resistant to |
Resistance to Deoxy(2)-2-iodoacetamideoglucose. |
PMID:6252281 |
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nagE in strain LR2-163, LR-12, LR-29 |
Resistant to |
Resistance to Streptozotocin |
PMID:6450313 |
STRAINS: LR2-163, LR-12, LR-29 |
table 2 | ||
nagE in strain LR2-163, LR-12, LR-29 |
Sensitivity to |
Sensitive to D-mannose |
PMID:6450313 |
table 2 | |||
nagE |
Resistant to |
Resistance to Iodoacetylglucosamine resistance |
PMID:4919471 |
Table 4 | |||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0665 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAATATTTTAGGTTTTTTCCA Primer 2:CCCTTTTTGATTTCATACAGCGG | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 92% [6] | ||
Linked marker |
est. P1 cotransduction: 1% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000628 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0629 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002312 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
NagE |
---|---|
Synonyms |
fused N-acetyl glucosamine specific PTS enzymes: IIC component[1], IIB component[1], IIA component[1], B0679[2][1], PstN[2][1], NagE[2][1] , ECK0667, JW0665, pstN, ptsN, b0679 |
Product description |
Component of N-acetylglucosamine PTS permease[2][3] N-acetylglucosamine-specific enzyme II of phosphotransferase system[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005215 |
transporter activity |
GO_REF:0000029 |
NAS: Non-traceable Author Statement |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005351 |
sugar:hydrogen symporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001127 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005351 |
sugar:hydrogen symporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001996 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005351 |
sugar:hydrogen symporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003352 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005351 |
sugar:hydrogen symporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010974 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001127 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001996 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0813 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008643 |
carbohydrate transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0762 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003352 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004719 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010974 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR011535 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008982 |
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:2.7.1.69 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001127 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001996 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003352 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004719 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010974 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR011535 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009401 |
phosphoenolpyruvate-dependent sugar phosphotransferase system |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0598 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001127 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001996 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003352 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0472 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GO_REF:0000029 |
NAS: Non-traceable Author Statement |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004719 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010974 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0812 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016301 |
kinase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0418 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0808 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016740 |
transferase activity |
GO_REF:0000029 |
NAS: Non-traceable Author Statement |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0019866 |
organelle inner membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR010974 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006974 |
response to DNA damage stimulus |
PMID:11967071 |
IEP: Inferred from Expression Pattern |
P |
complete | |||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of N-acetylglucosamine PTS permease |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
plasma membrane |
C-terminus localized in the cytoplasm with 11 predicted transmembrane domains |
Daley et al. (2005) [7] |
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plasma membrane |
From EcoCyc[3] |
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Inner Membrane |
PMID:3284790 |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MNILGFFQRL GRALQLPIAV LPVAALLLRF GQPDLLNVAF IAQAGGAIFD NLALIFAIGV ASSWSKDSAG AAALAGAVGY FVLTKAMVTI NPEINMGVLA GIITGLVGGA AYNRWSDIKL PDFLSFFGGK RFVPIATGFF CLVLAAIFGY VWPPVQHAIH AGGEWIVSAG ALGSGIFGFI NRLLIPTGLH QVLNTIAWFQ IGEFTNAAGT VFHGDINRFY AGDGTAGMFM SGFFPIMMFG LPGAALAMYF AAPKERRPMV GGMLLSVAVT AFLTGVTEPL EFLFMFLAPL LYLLHALLTG ISLFVATLLG IHAGFSFSAG AIDYALMYNL PAASQNVWML LVMGVIFFAI YFVVFSLVIR MFNLKTPGRE DKEDEIVTEE ANSNTEEGLT QLATNYIAAV GGTDNLKAID ACITRLRLTV ADSARVNDTM CKRLGASGVV KLNKQTIQVI VGAKAESIGD AMKKVVARGP VAAASAEATP ATAAPVAKPQ AVPNAVSIAE LVSPITGDVV ALDQVPDEAF ASKAVGDGVA VKPTDKIVVS PAAGTIVKIF NTNHAFCLET EKGAEIVVHM GIDTVALEGK GFKRLVEEGA QVSAGQPILE MDLDYLNANA RSMISPVVCS NIDDFSGLII KAQGHIVAGQ TPLYEIKK |
Length |
648 |
Mol. Wt |
68.348 kDa |
pI |
6.1 (calculated) |
Extinction coefficient |
50,880 - 51,505 (calc based on 12 Y, 6 W, and 5 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0002312 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000628 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0629 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MG1655 |
1222 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
213 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
352 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:703147..703187
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for nagE | |
microarray |
Summary of data for nagE from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
GFP Fusion |
Intergenic region (702739..703185) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
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Notes
Accessions Related to nagE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0629 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000628 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002312 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
NAGE |
From SHIGELLACYC |
E. coli O157 |
NAGE |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
PF00358 phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 |
||
EcoCyc:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10635 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000628 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0629 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002312 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
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