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Standard Name	nadC
Gene Synonym(s)	ECK0108, b0109, JW0105, nic^[1]^[2]
Product Desc.	quinolinate phosphoribosyltransferase (decarboxylating)^[2]^[3]; Component of quinolinate phosphoribosyltransferase (decarboxylating)^[2]^[3] Quinolinate phosphoribosyl transferase^[4]
Product Synonyms(s)	quinolinate phosphoribosyltransferase^[1], B0109^[2]^[1], Nic^[2]^[1], NadC^[2]^[1], nicotinate-nucleotide pyrophosphorylase (carboxylating)^[2]^[1], QAPRTase^[2]^[1], nicotinate-nucleotide:pyrophosphate phospho-α-D-ribosyltransferase (carboxylating)^[2]^[1] , ECK0108, JW0105, b0109
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nadC^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nadC
Mnemonic	Nicotinamide adenine dinucleotide
Synonyms	ECK0108, b0109, JW0105, nic^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	2.54 minutes	MG1655: 118645..117752 <gbrowseImage> name=NC_000913:117752..118645 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 121449..120556 <gbrowseImage> name=NC_012967:120556..121449 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 117751..118644 <gbrowseImage> name=NC_012759:117751..118644 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 118645..117752 <gbrowseImage> name=NC_007779:117752..118645 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 92749..91856 <gbrowseImage> name=NC_010473:91856..92749 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
nadC(del) (Keio:JW0105)	deletion	deletion	PMID:16738554	Shigen CGSC8387^[5]
nadC8				CGSC:5506
nadC13				CGSC:5614
nadC727(del)::kan			PMID:16738554	CGSC:101984
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0105	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCGCCTCGCCGCTATAACCC Primer 2:CCGCGAAAACGCATTGAAAGGTC
15B8	Kohara Phage	Genobase	PMID:3038334
4D12	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 25% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 30% ^[6]
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc	EcoCyc:EG11546	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001500	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948869	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1508	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000374	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NadC
Synonyms	quinolinate phosphoribosyltransferase^[1], B0109^[2]^[1], Nic^[2]^[1], NadC^[2]^[1], nicotinate-nucleotide pyrophosphorylase (carboxylating)^[2]^[1], QAPRTase^[2]^[1], nicotinate-nucleotide:pyrophosphate phospho-α-D-ribosyltransferase (carboxylating)^[2]^[1] , ECK0108, JW0105, b0109
Product description	quinolinate phosphoribosyltransferase (decarboxylating)^[2]^[3]; Component of quinolinate phosphoribosyltransferase (decarboxylating)^[2]^[3] Quinolinate phosphoribosyl transferase^[4]
EC number (for enzymes)	2.4.2.19^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity		ISS: Inferred from Sequence or Structural Similarity	UniProtKB:P30012	F	S. typhimurium NadC shown to have this activity (IDA). ^[7]	Missing: reference
	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity		ISS: Inferred from Sequence or Structural Similarity	UniProtKB:P30012	F	PMID:203571 nadC mutant of Salmonella typhimurium requires nicotinate for growth on minimal medium (IMP, Table 4).	Missing: reference
	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002638	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004393	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.4.2.19	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:8561507	IDA: Inferred from Direct Assay		C		complete
	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity	PMID:8561507	IDA: Inferred from Direct Assay		F		complete
	GO:0009435	NAD biosynthetic process	PMID:8561507	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of quinolinate phosphoribosyltransferase (decarboxylating)	could be indirect
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1135586
Protein	rpsS		PMID:19402753	LCMS(ID Probability):99.6
Protein	rplF		PMID:19402753	LCMS(ID Probability):99.6
Protein	dksA		PMID:19402753	LCMS(ID Probability):99.6
Protein	rplK		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPPRRYNPDT RRDELLERIN LDIPGAVAQA LREDLGGTVD ANNDITAKLL PENSRSHATV ITRENGVFCG KRWVEEVFIQ LAGDDVTIIW HVDDGDVINA NQSLFELEGP SRVLLTGERT ALNFVQTLSG VASKVRHYVE LLEGTNTQLL DTRKTLPGLR SALKYAVLCG GGANHRLGLS DAFLIKENHI IASGSVRQAV EKASWLHPDA PVEVEVENLE ELDEALKAGA DIIMLDNFET EQMREAVKRT NGKALLEVSG NVTDKTLREF AETGVDFISV GALTKHVQAL DLSMRFR
Length	297
Mol. Wt	32.761 kDa
pI	5.0 (calculated)
Extinction coefficient	20,970 - 21,220 (calc based on 3 Y, 3 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed (By similarity)	UniProt:P30011
Domain	131..295	PF01729 Quinolinate phosphoribosyl transferase, C-terminal domain		PMID:19920124
Domain	41..129	PF02749 Quinolinate phosphoribosyl transferase, N-terminal domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nadC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128102	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948869	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000374	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P30011	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11546	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11546	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948869	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001500	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1508	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	39.117+/-0.204	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.026315789	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1072	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	465	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	591	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nadC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:118625..118665 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0109 (EcoliWiki Page)	NCBI GEO profiles for nadC
microarray	GenExpDB:b0109 (EcoliWiki Page)	Summary of data for nadC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (118571..118804) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:B11^[8]
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Notes

Accessions Related to nadC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11546	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1508	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0109
EcoGene	EcoGene:EG11546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001500	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000374	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000002238 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT2G01350 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000024072 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000024925 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0231362 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000219771 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000015701 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000001766 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1914625 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000013614 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000023080 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YFR047C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000154070 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00032500001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000041000 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	NADC	From SHIGELLACYC
E. coli O157	NADC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02749 Quinolinate phosphoribosyl transferase, N-terminal domain
Pfam (EcoliWiki Page)	PF01729 Quinolinate phosphoribosyl transferase, C-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:51690
Panther (EcoliWiki Page)	PTHR11948:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:54675
EcoCyc	EcoCyc:EG11546	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001500	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1508	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000374	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Hughes, KT et al. (1993) The Salmonella typhimurium nadC gene: sequence determination by use of Mud-P22 and purification of quinolinate phosphoribosyltransferase. J. Bacteriol. 175 479-86 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

nadC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nadC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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