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Product(s) |

Expression|

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Standard Name	nadA
Gene Synonym(s)	ECK0739, b0750, JW0733, nicA^[1], nicA
Product Desc.	QUINOLINATE-SYNTHA^[2]^[3]; Component of quinolinate synthase complex^[2]^[3] Quinolinate synthase, A protein; has one [4Fe-4S] cluster per chain^[4]
Product Synonyms(s)	quinolinate synthase, subunit A^[1], B0750^[2]^[1], NicA^[2]^[1], NadA^[2]^[1] , ECK0739, JW0733, nicA, b0750
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nadA^[2]
Regulation/Activity
Quick Links
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Notes

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C291 and C294 form a reversible disulfide bond that regulates NadA activity (Saunders, 2008).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nadA
Mnemonic	Nicotinamide adenine dinucleotide
Synonyms	ECK0739, b0750, JW0733, nicA^[1], nicA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	16.84 minutes	MG1655: 781308..782351 <gbrowseImage> name=NC_000913:781308..782351 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 762714..763757 <gbrowseImage> name=NC_012967:762714..763757 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 684276..685319 <gbrowseImage> name=NC_012759:684276..685319 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 782507..783550 <gbrowseImage> name=NC_007779:782507..783550 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 833900..834943 <gbrowseImage> name=NC_010473:833900..834943 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔnadA (Keio:JW0733)	deletion	deletion		PMID:16738554	Shigen CGSC8797^[5]
nadAC294S		C294S	Activity is 13-fold lower under oxic conditions than under anoxic conditions			seeded from UniProt:P11458
nadAC291S		C291S	Activity is 3.3-fold lower under oxic conditions than under anoxic conditions			seeded from UniProt:P11458
nadA57::Tn10	Insertion at 781,532 bp in MG1655 (NC_000913)			adapted from Nichols et al.^[6]	CAG12147 = CGSC7351^[5]	Synonyms:
nadA50::Tn10					CGSC:7104
ΔnadA71					CGSC:10631
nadA5::Mu					CGSC:11552
nadA19					CGSC:11811
[nadA12]					CGSC:16902	null allele
nadA57::Tn10				PMID:2540407	CGSC:38015
nadA3052::Tn10kan				PMID:2540407	CGSC:38018
ΔnadA721::kan				PMID:16738554	CGSC:101980
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0733	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCGTAATGTTTGATCCAGA Primer 2:CCTCCACGTAGTGTAGCCGCAAA
10G5	Kohara Phage	Genobase	PMID:3038334
4H1	Kohara Phage	Genobase	PMID:3038334
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: 19% ^[6] Synonyms:zbi-29::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10630	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10630	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000623	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945351	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0624	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NadA
Synonyms	quinolinate synthase, subunit A^[1], B0750^[2]^[1], NicA^[2]^[1], NadA^[2]^[1] , ECK0739, JW0733, nicA, b0750
Product description	QUINOLINATE-SYNTHA^[2]^[3]; Component of quinolinate synthase complex^[2]^[3] Quinolinate synthase, A protein; has one [4Fe-4S] cluster per chain^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00567	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008987	quinolinate synthetase A activity	PMID:2841129	IMP: Inferred from Mutant Phenotype		F	Activity discussed in methodology.	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008987	quinolinate synthetase A activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00567	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008987	quinolinate synthetase A activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003473	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051539	4 iron, 4 sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0004	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of quinolinate synthase complex	could be indirect
Protein	kdsD		PMID:16606699	Experiment(s):EBI-1137852
Protein	accA		PMID:16606699	Experiment(s):EBI-1137852
Protein	nadE		PMID:16606699	Experiment(s):EBI-1137852
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSVMFDPDTA IYPFPPKPTP LSIDEKAYYR EKIKRLLKER NAVMVAHYYT DPEIQQLAEE TGGCISDSLE MARFGAKHPA STLLVAGVRF MGETAKILSP EKTILMPTLQ AECSLDLGCP VEEFNAFCDA HPDRTVVVYA NTSAAVKARA DWVVTSSIAV ELIDHLDSLG EKIIWAPDKH LGRYVQKQTG GDILCWQGAC IVHDEFKTQA LTRLQEEYPD AAILVHPESP QAIVDMADAV GSTSQLIAAA KTLPHQRLIV ATDRGIFYKM QQAVPDKELL EAPTAGEGAT CRSCAHCPWM AMNGLQAIAE ALEQEGSNHE VHVDERLRER ALVPLNRMLD FAATLRG
Length	347
Mol. Wt	38.24 kDa
pI	5.1 (calculated)
Extinction coefficient	35,410 - 36,535 (calc based on 9 Y, 4 W, and 9 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	30..341	PF02445 Quinolinate synthetase A protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nadA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128718	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945351	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002541	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P11458	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10630	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10630	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945351	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000623	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0624	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	5.71E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	4511	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1359	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	3709	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nadA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:781288..781328 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0750 (EcoliWiki Page)	NCBI GEO profiles for nadA
microarray	GenExpDB:b0750 (EcoliWiki Page)	Summary of data for nadA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nadA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10630	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0624	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0750
EcoGene	EcoGene:EG10630	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000623	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000029575 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G50210 (score: 1.000; bootstrap: 100%) AT1G67810 (score: 0.082)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000020754 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000034096 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	NADA	From SHIGELLACYC
E. coli O157	NADA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02445 Quinolinate synthetase A protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:142754
EcoCyc	EcoCyc:EG10630	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10630	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000623	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0624	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

nadA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nadA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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