mutM:Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
mutM |
|---|---|
| Mnemonic |
Mutator |
| Synonyms |
ECK3625, b3635, JW3610, fpg[1], fpg |
| edit table |
</protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
82.08 minutes |
MG1655: 3809175..3808366 |
||
|
NC_012967: 3749507..3748698 |
||||
|
NC_012759: 3696692..3697501 |
||||
|
W3110 |
|
W3110: 3829263..3830072 |
||
|
DH10B: 3906752..3905943 |
||||
| edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3808369 |
Edman degradation |
| ||
| edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
mutM(del) (Keio:JW3610) |
deletion |
deletion |
|||||
|
mutM::Tn5KAN-I-SceI (FB21352) |
Insertion at nt 312 in Plus orientation |
contains pKD46 | |||||
|
mutM::Tn5KAN-I-SceI (FB21353) |
Insertion at nt 312 in Plus orientation |
does not contain pKD46 | |||||
|
mutMK218T |
K218T |
Slight increase of substrate affinity and decrease of activity |
seeded from UniProt:P05523 | ||||
|
mutME174Q |
E174Q |
Strong decrease of glycosylase activity; slight decrease of AP lyase activity |
seeded from UniProt:P05523 | ||||
|
mutME3Q |
E3Q |
Strong decrease of glycosylase activity; slight decrease of AP lyase activity |
seeded from UniProt:P05523 | ||||
|
mutME6Q |
E6Q |
Decrease of glycosylase activity; no effect on AP lyase activity |
seeded from UniProt:P05523 | ||||
|
mutMH90A |
H90A |
Increase of substrate affinity and decrease of activity |
seeded from UniProt:P05523 | ||||
|
mutMD107N |
D107N |
No effect on glycosylase and AP lyase activity |
seeded from UniProt:P05523 | ||||
|
mutMR109A |
R109A |
Over 100-fold decrease of activity |
seeded from UniProt:P05523 | ||||
|
mutMR110A |
R110A |
Over 100-fold increase of substrate affinity and decrease of activity |
seeded from UniProt:P05523 | ||||
|
mutME132Q |
E132Q |
Decrease of glycosylase activity; slight decrease of AP lyase activity |
seeded from UniProt:P05523 | ||||
|
mutMD160N |
D160N |
No effect on glycosylase and AP lyase activity |
seeded from UniProt:P05523 | ||||
|
mutM102::kan |
|||||||
|
mutM103::mini-Tn10 |
| ||||||
| edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3610 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCCTGAATTACCCGAAGTTGA Primer 2:CCtTTCTGGCACTGCCGACAATA | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
zhg-50::Tn10 |
Linked marker |
est. P1 cotransduction: % [9] | |
|
zic-4901::Tn10 |
Linked marker |
est. P1 cotransduction: 50% [9] | |
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
<protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Boiteux, S et al. (1987) Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein. EMBO J. 6 3177-83 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 5.0 5.1 Bridges, BA et al. (1996) Effect of mutY and mutM/fpg-1 mutations on starvation-associated mutation in Escherichia coli: implications for the role of 7,8-dihydro-8-oxoguanine. Mol. Gen. Genet. 251 352-7 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
