murE:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
murE |
---|---|
Gene Synonym(s) |
ECK0086, b0085, JW0083[1], JW0083 |
Product Desc. |
UDP- meso-diaminopimelate adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase[2] |
Product Synonyms(s) |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase[1], B0085[3][1], MurE[3][1] , ECK0086, JW0083, b0085 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression |
transcription unit(s): mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ[3] |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
A carbamylated lysine residue is present in the active site.[2]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
murE |
---|---|
Mnemonic |
Murein |
Synonyms |
ECK0086, b0085, JW0083[1], JW0083 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
2.01 minutes |
MG1655: 93166..94653 |
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NC_012967: 95970..97457 |
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NC_012759: 93165..94652 |
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W3110 |
|
W3110: 93166..94653 |
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DH10B: 67270..68757 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
93169 |
Edman degradation |
PMID:2198024 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
murEA495S |
A495S |
(in murE1) |
Strain variation; seeded from UniProt:P22188 | ||||
murEE344K |
E344K |
(in murE1) |
Strain variation; seeded from UniProt:P22188 | ||||
murE1 |
|||||||
ispA Pxyl-murE-B |
Growth Phenotype |
repression of the murE-B operon allowed vigorous L-form growth. |
PMID:23452849 |
Mutant Strain: LR2 |
Repression in Figure 2A | ||
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Notes
Sequence of the murE1 allele [4]. A strain with the murE1 is temperature sensitive for growth. Mutants grow at 30C, but lyse at 42C. [5]
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0083 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGCAGATCGTAATTTGCGCGA Primer 2:CCTGCAATCACCCCCAGCAGACG | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 72% [7] | |
Linked marker |
est. P1 cotransduction: 7% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000614 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0616 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0000311 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
MurE |
---|---|
Synonyms |
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase[1], B0085[3][1], MurE[3][1] , ECK0086, JW0083, b0085 |
Product description |
UDP- meso-diaminopimelate adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso-diaminopimelate ligase[2] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00208 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008765 |
UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity |
PMID:2269304 |
IDA: Inferred from Direct Assay |
F |
complete | |||
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000713 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004101 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005761 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013221 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00208 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005761 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007047 |
cellular cell wall organization |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0961 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007049 |
cell cycle |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0131 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008360 |
regulation of cell shape |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005761 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008360 |
regulation of cell shape |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0133 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008765 |
UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:6.3.2.13 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009058 |
biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000713 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009058 |
biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004101 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009058 |
biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013221 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009252 |
peptidoglycan biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00208 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009252 |
peptidoglycan biosynthetic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005761 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009252 |
peptidoglycan biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0573 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051301 |
cell division |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005761 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051301 |
cell division |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0132 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
dnaK |
PMID:16606699 |
Experiment(s):EBI-1135536 | |
Protein |
nadE |
PMID:16606699 |
Experiment(s):EBI-1135536 | |
Protein |
hyfB |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
katG |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
pepP |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
pnp |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
rpsB |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
rseP |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
ydaY |
PMID:15690043 |
Experiment(s):EBI-894085 | |
Protein |
ftsE |
PMID:19402753 |
MALDI(Z-score):36.340065 | |
Protein |
fusA |
PMID:19402753 |
MALDI(Z-score):29.057010 | |
Protein |
rpsJ |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rfaD |
PMID:19402753 |
MALDI(Z-score):37.417484 | |
Protein |
hyfB |
PMID:19402753 |
LCMS(ID Probability):99.0 | |
Protein |
rseP |
PMID:19402753 |
LCMS(ID Probability):99.0 | |
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</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MADRNLRDLL APWVPDAPSR ALREMTLDSR VAAAGDLFVA VVGHQADGRR YIPQAIAQGV AAIIAEAKDE ATDGEIREMH GVPVIYLSQL NERLSALAGR FYHEPSDNLR LVGVTGTNGK TTTTQLLAQW SQLLGEISAV MGTVGNGLLG KVIPTENTTG SAVDVQHELA GLVDQGATFC AMEVSSHGLV QHRVAALKFA ASVFTNLSRD HLDYHGDMEH YEAAKWLLYS EHHCGQAIIN ADDEVGRRWL AKLPDAVAVS MEDHINPNCH GRWLKATEVN YHDSGATIRF SSSWGDGEIE SHLMGAFNVS NLLLALATLL ALGYPLADLL KTAARLQPVC GRMEVFTAPG KPTVVVDYAH TPDALEKALQ AARLHCAGKL WCVFGCGGDR DKGKRPLMGA IAEEFADVAV VTDDNPRTEE PRAIINDILA GMLDAGHAKV MEGRAEAVTC AVMQAKENDV VLVAGKGHED YQIVGNQRLD YSDRVTVARL LGVIA |
Length |
495 |
Mol. Wt |
53.342 kDa |
pI |
5.6 (calculated) |
Extinction coefficient |
54,890 - 55,890 (calc based on 11 Y, 7 W, and 8 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0000311 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000614 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0616 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
1.73E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
Ecoli K-12 |
31.722+/-0.115 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.051782112 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
1147 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
398 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
1007 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:93146..93186
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for murE | |
microarray |
Summary of data for murE from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to murE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0616 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000614 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0000311 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Shigella flexneri |
MURE |
From SHIGELLACYC |
E. coli O157 |
MURE |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10621 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000614 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0616 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0000311 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Eveland, SS et al. (1997) Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily. Biochemistry 36 6223-9 PubMed
- ↑ Lugtenberg, EJ & v Schijndel-van Dam, A (1972) Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme. J. Bacteriol. 110 35-40 PubMed
- ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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