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Gene |

Product(s) |

Expression|

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Standard Name	msyB
Gene Synonym(s)	ECK1037, b1051, JW1039^[1], JW1039
Product Desc.	acidic protein that suppresses mutants lacking function of protein export^[2]^[3] In multicopy restores growth and protein export functions of secY and secA mutants^[4]
Product Synonyms(s)	predicted protein^[1], B1051^[2]^[1], MsyB^[2]^[1] , ECK1037, JW1039, b1051
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): msyB^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	msyB
Mnemonic	Multicopy suppressor of secY
Synonyms	ECK1037, b1051, JW1039^[1], JW1039
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	23.99 minutes	MG1655: 1113404..1113030 <gbrowseImage> name=NC_000913:1113030..1113404 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1128750..1128376 <gbrowseImage> name=NC_012967:1128376..1128750 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1016955..1017329 <gbrowseImage> name=NC_012759:1016955..1017329 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1115758..1115384 <gbrowseImage> name=NC_007779:1115384..1115758 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1168849..1168475 <gbrowseImage> name=NC_010473:1168475..1168849 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔmsyB (Keio:JW1039)	deletion	deletion	PMID:16738554	Shigen CGSC9002^[5]
msyB::Tn5KAN-2 (FB20093)	Insertion at nt 255 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20093	does not contain pKD46
ΔmsyB722::kan			PMID:16738554	CGSC:101960
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1039	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACCATGTACGCAACGCTTGA Primer 2:CCACGTTCATCCCACTCATCAGC
1H7	Kohara Phage	Genobase	PMID:3038334
E4H10S	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: 9% ^[6]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 16% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11338	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11338	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001309	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945612	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1314	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003567	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MsyB
Synonyms	predicted protein^[1], B1051^[2]^[1], MsyB^[2]^[1] , ECK1037, JW1039, b1051
Product description	acidic protein that suppresses mutants lacking function of protein export^[2]^[3] In multicopy restores growth and protein export functions of secY and secA mutants^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	pepD	PMID:16606699	Experiment(s):EBI-1138694
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1138694
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1138694
Protein	rplB	PMID:16606699	Experiment(s):EBI-1138694
Protein	rpmB	PMID:16606699	Experiment(s):EBI-1138694
Protein	tatE	PMID:16606699	Experiment(s):EBI-1138694
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1138694
Protein	stpA	PMID:16606699	Experiment(s):EBI-1138694
Protein	rpsG	PMID:16606699	Experiment(s):EBI-1138694
Protein	dnaN	PMID:19402753	MALDI(Z-score):18.769963
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMTMYATLEE AIDAAREEFL ADNPGIDAED ANVQQFNAQK YVLQDGDIMW QVEFFADEGE EGECLPMLSG EAAQSVFDGD YDEIEIRQEW QEENTLHEWD EGEFQLEPPL DTEEGRAAAD EWDER
Length	125
Mol. Wt	14.389 kDa
pI	3.4 (calculated)
Extinction coefficient	26,470 - 26,595 (calc based on 3 Y, 4 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=msyB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111208	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945612	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003567	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P25738	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11338	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11338	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945612	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001309	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1314	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	6.13E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	402	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	5249	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	334	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
msyB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1113384..1113424 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1051 (EcoliWiki Page)	NCBI GEO profiles for msyB
microarray	GenExpDB:b1051 (EcoliWiki Page)	Summary of data for msyB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to msyB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11338	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1314	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1051
EcoGene	EcoGene:EG11338	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001309	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003567	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
E. coli O157	MSYB	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

msyB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to msyB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Families

Notes

Links

References

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