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Standard Name	mreC
Gene Synonym(s)	ECK3238, b3250, JW3219^[1], JW3219
Product Desc.	MreC^[2]^[3]; Component of longitudinal peptidoglycan synthesis/chromosome segregation-directing complex^[3] Cell division and growth; mecillinam resistance; rod shape-determining protein^[4]
Product Synonyms(s)	cell wall structural complex MreBCD transmembrane component MreC^[1], B3250^[2]^[1], MreC^[2]^[1] , ECK3238, JW3219, b3250
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mreBCD-yhdE-rng^[2]
Regulation/Activity
Quick Links
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Notes

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In operon with mreBD and the gene products form an essential membrane-bound complex responsible for cell morphology^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mreC
Mnemonic	Murein cluster e; mecillinam resistance
Synonyms	ECK3238, b3250, JW3219^[1], JW3219
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	73.21 minutes	MG1655: 3398000..3396897 <gbrowseImage> name=NC_000913:3396897..3398000 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3327869..3326766 <gbrowseImage> name=NC_012967:3326766..3327869 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3284045..3285148 <gbrowseImage> name=NC_012759:3284045..3285148 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3399833..3398730 <gbrowseImage> name=NC_007779:3398730..3399833 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
Strain DH10B	L17Q		Nonsynonomous mutation	PMID:18245285	ASAP	This is a SNP in E. coli K-12 Strain DH10B
mre-129		Resistant to	Mecillinam resistance at 42C	PMID:2822655 & ^[5]	Strain: AT1325-678	table 2
mre-678		Cell Shape	Sphere shaped	PMID:2822655 & ^[6]	Strain: AT1325-678	table 2
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3219	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAGCCAATTTTTAGCCGTGG Primer 2:CCTTGCCCTCCCGGCGCACGCGC
21D3	Kohara Phage	Genobase	PMID:3038334
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[7]	est. P1 cotransduction: 27% ^[8] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[7]	est. P1 cotransduction: 53% ^[8] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10609	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10609	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000602	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947655	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0604	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010658	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MreC
Synonyms	cell wall structural complex MreBCD transmembrane component MreC^[1], B3250^[2]^[1], MreC^[2]^[1] , ECK3238, JW3219, b3250
Product description	MreC^[2]^[3]; Component of longitudinal peptidoglycan synthesis/chromosome segregation-directing complex^[3] Cell division and growth; mecillinam resistance; rod shape-determining protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008360	regulation of cell shape	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005223	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007221	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0133	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of longitudinal peptidoglycan synthesis/chromosome segregation-directing complex	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKPIFSRGPS LQIRLILAVL VALGIIIADS RLGTFSQIRT YMDTAVSPFY FVSNAPRELL DGVSQTLASR DQLELENRAL RQELLLKNSE LLMLGQYKQE NARLRELLGS PLRQDEQKMV TQVISTVNDP YSDQVVIDKG SVNGVYEGQP VISDKGVVGQ VVAVAKLTSR VLLICDATHA LPIQVLRNDI RVIAAGNGCT DDLQLEHLPA NTDIRVGDVL VTSGLGGRFP EGYPVAVVSS VKLDTQRAYT VIQARPTAGL QRLRYLLLLW GADRNGANPM TPEEVHRVAN ERLMQMMPQV LPSPDAMGPK LPEPATGIAQ PTPQQPATGN AATAPAAPTQ PAANRSPQRA TPPQSGAQPP ARAPGGQ
Length	367
Mol. Wt	39.53 kDa
pI	8.6 (calculated)
Extinction coefficient	17,420 - 17,670 (calc based on 8 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	121..273	PF04085 rod shape-determining protein MreC		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mreC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131138	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947655	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010658	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P16926	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10609	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10609	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947655	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000602	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0604	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	738	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	176	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	333	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mreBCD-yhdE-rng
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3397980..3398020 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3250 (EcoliWiki Page)	NCBI GEO profiles for mreC
microarray	GenExpDB:b3250 (EcoliWiki Page)	Summary of data for mreC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3397535..3397716) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:H11^[9]
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Notes

Accessions Related to mreC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10609	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0604	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3250
EcoGene	EcoGene:EG10609	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000602	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010658	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000030618 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000002890 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MREC	From SHIGELLACYC
E. coli O157	MREC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04085 rod shape-determining protein MreC
EcoCyc	EcoCyc:EG10609	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10609	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000602	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0604	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010658	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Okada, Y., M. Eachi,K. Nagai, and M. Matsihashi. 1992. Chage of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB mreC mreD of Escherichia coli. J. Gen. Appl. Microbiol. 38:157-167.
↑ Okada, Y., M. Eachi,K. Nagai, and M. Matsihashi. 1992. Chage of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB mreC mreD of Escherichia coli. J. Gen. Appl. Microbiol. 38:157-167.
↑ ^7.0 ^7.1 CGSC: The Coli Genetics Stock Center
↑ ^8.0 ^8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

mreC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mreC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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