mreC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mreC

Mnemonic

Murein cluster e; mecillinam resistance

Synonyms

ECK3238, b3250, JW3219[1], JW3219

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

73.21 minutes 

MG1655: 3398000..3396897
<gbrowseImage> name=NC_000913:3396897..3398000 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3327869..3326766
<gbrowseImage> name=NC_012967:3326766..3327869 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3284045..3285148
<gbrowseImage> name=NC_012759:3284045..3285148 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3399833..3398730
<gbrowseImage> name=NC_007779:3398730..3399833 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

L17Q

Nonsynonomous mutation

PMID:18245285[2]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

mre-129

Resistant to

Mecillinam resistance at 42C

PMID:2822655[3] & [4]

Strain: AT1325-678

table 2

mre-678

Cell Shape

Sphere shaped

PMID:2822655[3] & [5]

Strain: AT1325-678

table 2

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3219

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAGCCAATTTTTAGCCGTGG

Primer 2:CCTTGCCCTCCCGGCGCACGCGC

21D3

Kohara Phage

Genobase

PMID:3038334[7]

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[8]

est. P1 cotransduction: 27% [9]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[8]

est. P1 cotransduction: 53% [9]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10609

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10609

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000602

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947655

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0604

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010658

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  3. 3.0 3.1 Wachi, M et al. (1987) Mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam, and amount of penicillin-binding proteins in Escherichia coli. J. Bacteriol. 169 4935-40 PubMed
  4. Okada, Y., M. Eachi,K. Nagai, and M. Matsihashi. 1992. Chage of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB mreC mreD of Escherichia coli. J. Gen. Appl. Microbiol. 38:157-167.
  5. Okada, Y., M. Eachi,K. Nagai, and M. Matsihashi. 1992. Chage of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB mreC mreD of Escherichia coli. J. Gen. Appl. Microbiol. 38:157-167.
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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