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Standard Name	mrdB
Gene Synonym(s)	ECK0627, b0634, JW0629, rodA, mdrB^[1]
Product Desc.	rod shape-determining membrane protein; sensitivity to radiation and drugs^[2]^[3] Affects cell shape, mecillinam sensitivity; recruits cognate transpetidase MrdA; SEDS protein^[4]
Product Synonyms(s)	cell wall shape-determining protein^[1], B0634^[2]^[1], RodA^[2]^[1], MdrB^[2]^[1], MrdB^[2]^[1] , ECK0627, JW0629, rodA, b0634
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rlpA^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mrdB
Mnemonic	Murein cluster d
Synonyms	ECK0627, b0634, JW0629, rodA, mdrB^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	14.32 minutes	MG1655: 665536..664424 <gbrowseImage> name=NC_000913:664424..665536 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 648897..647785 <gbrowseImage> name=NC_012967:647785..648897 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 567184..568296 <gbrowseImage> name=NC_012759:567184..568296 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 666735..665623 <gbrowseImage> name=NC_007779:665623..666735 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 604868..603756 <gbrowseImage> name=NC_010473:603756..604868 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 718128..717016 <gbrowseImage> name=NC_010473:717016..718128 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
mrdB51				CGSC:7212
mrdB4	Resistant to	Resistant to Mecillinam at 42C. WT at 30C.	PMID:6243629	Strain: TMM4	Strain TMM6 (mrdA6) has the exact same phenotype
mrdB4	Cell Shape	Cell shape is spherical compared to the rod shaped wild type	PMID:6243629	Strain: TMM4	Strain mutant TMM6 (mrdB6) has the exact same phenotype
rodX^- in strains H3, HL29, HL103	Resistant to	Resistance to mecillinam	PMID:201607	strains: H3, HL29, HL103	rodX is a suggested synonym of rodA by this paper as well as Iwaya et. al. ^[5]
rodX^- in strains H3, HL29, HL103	Colony Morphology	Round morphology	PMID:201607	strains: H3, HL29, HL103	rodX is a suggested synonym of rodA by this paper as well as Iwaya et. al. ^[5]
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0629	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACGGATAATCCGAATAAAAA Primer 2:CCCACGCTTTTCGACAACATTTT
1G6	Kohara Phage	Genobase	PMID:3038334
15D7	Kohara Phage	Genobase	PMID:3038334
crcA280::Tn10	Linked marker	CAG12077 = CGSC7347^[6]	est. P1 cotransduction: 70% ^[7] Synonyms:zbe-280::Tn10
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[6]	est. P1 cotransduction: 23% ^[7] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10607	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10607	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000600	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945238	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0602	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002173	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MrdB
Synonyms	cell wall shape-determining protein^[1], B0634^[2]^[1], RodA^[2]^[1], MdrB^[2]^[1], MrdB^[2]^[1] , ECK0627, JW0629, rodA, b0634
Product description	rod shape-determining membrane protein; sensitivity to radiation and drugs^[2]^[3] Affects cell shape, mecillinam sensitivity; recruits cognate transpetidase MrdA; SEDS protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	PMID:6451612	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001182	P	Seeded from EcoCyc (v14.0)	complete
	GO:0007049	cell cycle	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018365	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011923	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0133	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001182	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011923	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018365	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence
plasma membrane	C-terminus localized in the cytoplasm with 9 predicted transmembrane domains	Daley et al. (2005) ^[8]
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTDNPNKKTF WDKVHLDPTM LLILLALLVY SALVIWSASG QDIGMMERKI GQIAMGLVIM VVMAQIPPRV YEGWAPYLYI ICIILLVAVD AFGAISKGAQ RWLDLGIVRF QPSEIAKIAV PLMVARFINR DVCPPSLKNT GIALVLIFMP TLLVAAQPDL GTSILVALSG LFVLFLSGLS WRLIGVAVVL VAAFIPILWF FLMHDYQRQR VMMLLDPESD PLGAGYHIIQ SKIAIGSGGL RGKGWLHGTQ SQLEFLPERH TDFIFAVLAE ELGLVGILIL LALYILLIMR GLWIAARAQT TFGRVMAGGL MLILFVYVFV NIGMVSGILP VVGVPLPLVS YGGSALIVLM AGFGIVMSIH THRKMLSKSV
Length	370
Mol. Wt	40.477 kDa
pI	9.7 (calculated)
Extinction coefficient	57,410 - 57,660 (calc based on 9 Y, 8 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	17..365	PF01098 Cell cycle protein		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mrdB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128617	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945238	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002173	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABG7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10607	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10607	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945238	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000600	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0602	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	435	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	103	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	156	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rlpA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:665516..665556 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0634 (EcoliWiki Page)	NCBI GEO profiles for mrdB
microarray	GenExpDB:b0634 (EcoliWiki Page)	Summary of data for mrdB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mrdB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10607	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0602	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0634
EcoGene	EcoGene:EG10607	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000600	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MRDB	From SHIGELLACYC
E. coli O157	MRDB	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01098 Cell cycle protein
EcoCyc	EcoCyc:EG10607	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10607	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000600	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0602	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 Iwaya, M et al. (1978) Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape. J. Bacteriol. 136 1143-58 PubMed
↑ ^6.0 ^6.1 CGSC: The Coli Genetics Stock Center
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

mrdB:On One Page

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Gene

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Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Notes

Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

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Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

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Experimental

Notes

Accessions Related to mrdB Expression

Notes

Links

Evolution

Homologs in Other Organisms

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Notes

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Notes

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