mrdB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mrdB

Mnemonic

Murein cluster d

Synonyms

ECK0627, b0634, JW0629, rodA, mdrB[1]

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

14.32 minutes 

MG1655: 665536..664424
<gbrowseImage> name=NC_000913:664424..665536 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 648897..647785
<gbrowseImage> name=NC_012967:647785..648897 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 567184..568296
<gbrowseImage> name=NC_012759:567184..568296 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 666735..665623
<gbrowseImage> name=NC_007779:665623..666735 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 604868..603756
<gbrowseImage> name=NC_010473:603756..604868 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 718128..717016
<gbrowseImage> name=NC_010473:717016..718128 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mrdB51

CGSC:7212

mrdB4

Resistant to

Resistant to Mecillinam at 42C. WT at 30C.

PMID:6243629[2]

Strain: TMM4

Strain TMM6 (mrdA6) has the exact same phenotype

mrdB4

Cell Shape

Cell shape is spherical compared to the rod shaped wild type

PMID:6243629[2]

Strain: TMM4

Strain mutant TMM6 (mrdB6) has the exact same phenotype

rodX - in strains H3, HL29, HL103

Resistant to

Resistance to mecillinam

PMID:201607[3]

strains: H3, HL29, HL103

rodX is a suggested synonym of rodA by this paper as well as Iwaya et. al. [4]

rodX - in strains H3, HL29, HL103

Colony Morphology

Round morphology

PMID:201607[3]

strains: H3, HL29, HL103

rodX is a suggested synonym of rodA by this paper as well as Iwaya et. al. [4]

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0629

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACGGATAATCCGAATAAAAA

Primer 2:CCCACGCTTTTCGACAACATTTT

1G6

Kohara Phage

Genobase

PMID:3038334[6]

15D7

Kohara Phage

Genobase

PMID:3038334[6]

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[7]

est. P1 cotransduction: 70% [8]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[7]

est. P1 cotransduction: 23% [8]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10607

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10607

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000600

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945238

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0602

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002173

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Tamaki, S et al. (1980) Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli. J. Bacteriol. 141 52-7 PubMed
  3. 3.0 3.1 Iwaya, M et al. (1978) Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli. J. Bacteriol. 133 196-202 PubMed
  4. 4.0 4.1 Iwaya, M et al. (1978) Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape. J. Bacteriol. 136 1143-58 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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