molR 3:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

molR_3

Gene Synonym(s)

molR, ECK2108, b2115, JW2102, yehF, yehG, b2116, JW5915, b2117, JW5916, b4499, JW2102+JW5915+JW5916, molR_1, yehH, molR_2[1][2][3], dinO, sosF

Product Desc.

molybdate metabolism regulator, interrupted[3][2]; twice split molybdate metabolism regulator[3][2]

Product Synonyms(s)

B2115[3], MolR_1[3], MolR[3], YehH[3], YehG[3], YehF[3], B2116[3] , dinO, ECK2108, JW2102, JW5915, JW5916, molR, sosF, yehF, yehG, b4499

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): molR_1[3], molR

Regulation/Activity
Quick Links

porteco.png regulondb.jpg

dnadisplay.png pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

molR_3

Mnemonic
Synonyms

molR, ECK2108, b2115, JW2102, yehF, yehG, b2116, JW5915, b2117, JW5916, b4499, JW2102+JW5915+JW5916, molR_1, yehH, molR_2[1][2][3], dinO, sosF

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

47.3 minutes 

MG1655: 2194496..2195320
<gbrowseImage> name=NC_000913:2194496..2195320 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2147671..2151465
<gbrowseImage> name=NC_012967:2147671..2151465 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2199808..2200632
<gbrowseImage> name=NC_007779:2199808..2200632 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

47.32 minutes, 47.32 minutes 

MG1655: 2195432..2197369
<gbrowseImage> name=NC_000913:2195432..2197369 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2200744..2202681
<gbrowseImage> name=NC_007779:2200744..2202681 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

47.36 minutes 

MG1655: 2197344..2198291
<gbrowseImage> name=NC_000913:2197344..2198291 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2202656..2203603
<gbrowseImage> name=NC_007779:2202656..2203603 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

 

MG1655: 2194496..2198291
<gbrowseImage> name=NC_000913:2194496..2198291 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2199808..2203603
<gbrowseImage> name=NC_007779:2199808..2203603 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

molR_1::Tn5KAN-2 (FB20621)

Insertion at nt 80 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20621

does not contain pKD46

molR_1::Tn5KAN-2 (FB20622)

Insertion at nt 80 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20622

contains pKD46

ΔmolR739::kan

PMID:16738554

CGSC:101926


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

3E4

Kohara Phage

Genobase

PMID:3038334

2B4

Kohara Phage

Genobase

PMID:3038334

17A4

Kohara Phage

Genobase

PMID:3038334

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[4]

est. P1 cotransduction: 46% [5]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[4]

est. P1 cotransduction: % [5]
Synonyms:zeg-722, zei-722::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:2847726

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004459

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MolR

MolR_2

Synonyms

B2115[3], MolR_1[3], MolR[3], YehH[3], YehG[3], YehF[3], B2116[3] , dinO, ECK2108, JW2102, JW5915, JW5916, molR, sosF, yehF, yehG, b4499

MolR_3[3], B2117[3]

Product description

molybdate metabolism regulator, interrupted[3][2]; twice split molybdate metabolism regulator[3][2]

twice split molybdate metabolism regulator[3][2]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology

molybdate metabolism regulator, interrupted

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status


Interactions

molybdate metabolism regulator, interrupted

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

groL

PMID:16606699

Experiment(s):EBI-1141967

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

 

at EcoCyc

 
Length
Mol. Wt
pI
Extinction coefficient



Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..71

PF05406 WGR domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=molR_3 taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GeneID:2847726

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0006999

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0007001

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0285100

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P33345

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:2847726

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004459

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

molR_1

Figure courtesy of RegulonDB

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Notes

LexA- regulated [6]

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2194476..2194516 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2117 (EcoliWiki Page)

NCBI GEO profiles for molR_3

microarray

GenExpDB:b2117 (EcoliWiki Page)

Summary of data for molR_3 from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2195230..2195474) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ07; Well:F5[7]

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Notes

Accessions Related to molR_3 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2117

RegulonDB

RegulonDB:ECK120004459

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

E. coli O157

MOLR_D

From ECOO157CYC

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Notes

Families

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Database Accession Notes

Pfam (EcoliWiki Page)

PF05406 WGR domain


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. Fernández De Henestrosa, AR et al. (2000) Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol. Microbiol. 35 1560-72 PubMed
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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