mhpF:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

mhpF

Gene Synonym(s)

ECK0348, b0351, JW0342[1], JW0342

Product Desc.

acetaldehyde dehydrogenase 2[2][3]

Acetaldehyde dehydrogenase, acylating; utilizes MHP[4]

Product Synonyms(s)

acetaldehyde-CoA dehydrogenase II, NAD-binding[1], B0351[2][1], MhpF[2][1] , ECK0348, JW0342, b0351

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mhpABCDFE[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mhpF

Mnemonic

m-Hydroxyphenylpropionic acid

Synonyms

ECK0348, b0351, JW0342[1], JW0342

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

8.02 minutes 

MG1655: 372145..373095
<gbrowseImage> name=NC_000913:372145..373095 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 345477..346427
<gbrowseImage> name=NC_012967:345477..346427 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 274904..275854
<gbrowseImage> name=NC_012759:274904..275854 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 372145..373095
<gbrowseImage> name=NC_007779:372145..373095 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mhpF(del) (Keio:JW0342)

deletion

deletion

PMID:16738554

Shigen

CGSC8532[5]

mhpF720(del)::kan

PMID:16738554

CGSC:101864


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0342

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTAAGCGTAAAGTCGCCAT

Primer 2:CCTGCCGCTTCTCCTGCCTTGCG

8F10

Kohara Phage

Genobase

PMID:3038334

mhpC281::Tn10

Linked marker

CAG12080 = CGSC7333[5]

est. P1 cotransduction: 97% [6]
Synonyms:zah-281::Tn10

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[5]

est. P1 cotransduction: 44% [6]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:M014

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004473

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945008

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3390

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001207

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MhpF

Synonyms

acetaldehyde-CoA dehydrogenase II, NAD-binding[1], B0351[2][1], MhpF[2][1] , ECK0348, JW0342, b0351

Product description

acetaldehyde dehydrogenase 2[2][3]

Acetaldehyde dehydrogenase, acylating; utilizes MHP[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:16782065

IPI: Inferred from Physical Interaction

UniProtKB:P51020

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000534

C

Seeded from EcoCyc (v14.0)

complete

GO:0006520

cellular amino acid metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000534

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc (v14.0)

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01657

F

Seeded from EcoCyc (v14.0)

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003361

F

Seeded from EcoCyc (v14.0)

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015426

F

Seeded from EcoCyc (v14.0)

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.2.1.10

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:16782065

IPI: Inferred from Physical Interaction

EcoliWiki:mhpE


F

complete

GO:0016620

oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000534

F

Seeded from EcoCyc (v14.0)

complete

GO:0008774

acetaldehyde dehydrogenase (acetylating) activity

PMID:6345511

IDA: Inferred from Direct Assay

F

complete

GO:0019439

aromatic compound catabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01657

P

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003361

P

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015426

P

Seeded from EcoCyc (v14.0)

complete

GO:0019439

aromatic compound catabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0058

P

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000534

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003361

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015426

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1136338

Protein

glpD

PMID:16606699

Experiment(s):EBI-1136338

Protein

nadE

PMID:16606699

Experiment(s):EBI-1136338
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRM GVATTHEGVI
GLMNMPEFAD IDIVFDATSA GAHVKNDAAL REAKPDIRLI DLTPAAIGPY CVPVVNLEAN
VDQLNVNMVT CGGQATIPMV AAVSRVARVH YAEIIASIAS KSAGPGTRAN IDEFTETTSR
AIEVVGGAAK GKAIIVLNPA EPPLMMRDTV YVLSDEASQD DIEASINEMA EAVQAYVPGY
RLKQRVQFEV IPQDKPVNLP GVGQFSGLKT AVWLEVEGAA HYLPAYAGNL DIMTSSALAT
AEKMAQSLAR KAGEAA
Length

316

Mol. Wt

33.442 kDa

pI

5.3 (calculated)

Extinction coefficient

15,930 - 16,180 (calc based on 7 Y, 1 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

5..123

PF01118 Semialdehyde dehydrogenase, NAD binding domain

PMID:19920124

Domain

131..284

PF09290 Prokaryotic acetaldehyde dehydrogenase, dimerisation

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mhpF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128336

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945008

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001207

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P77580

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:M014

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13625

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945008

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004473

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3390

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

18.658+/-0.128

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.008947368

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

14

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

15

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mhpABCDFE

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:372125..372165 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0351 (EcoliWiki Page)

NCBI GEO profiles for mhpF

microarray

GenExpDB:b0351 (EcoliWiki Page)

Summary of data for mhpF from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to mhpF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:M014

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3390

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0351

EcoGene

EcoGene:EG13625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004473

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001207

Escherichia coli str. K-12 substr. MG1655

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000014140 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

E. coli O157

MHPF

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:51735

Panther (EcoliWiki Page)

PTHR21123:SF0

Superfamily (EcoliWiki Page)

SUPERFAMILY:55347

Pfam (EcoliWiki Page)

PF01118 Semialdehyde dehydrogenase, NAD binding domain

Pfam (EcoliWiki Page)

PF09290 Prokaryotic acetaldehyde dehydrogenase, dimerisation

EcoCyc

EcoCyc:M014

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13625

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004473

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3390

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001207

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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