mglA:On One Page

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

<protect>

Standard Name

mglA

Gene Synonym(s)

ECK2142, b2149, JW2136, mglP, PMG, mgl[1]

Product Desc.

MglA[2][3];

Component of galactose ABC transporter[2][3]

Methyl-galactoside transporter[4]

Product Synonyms(s)

fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components[1], B2149[2][1], MglP[2][1], Mgl[2][1], MglA[2][1] , ECK2142, JW2136, mglP, b2149

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mglAC[2], mglBAC[2], OP00230, mglBAK

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]


Binds TrxA (Kumar, 2004).[4]



Gene

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mglA

Mnemonic

Methyl-galactoside

Synonyms

ECK2142, b2149, JW2136, mglP, PMG, mgl[1]

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

48.19 minutes 

MG1655: 2237311..2235791
<gbrowseImage> name=NC_000913:2235791..2237311 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2190527..2189007
<gbrowseImage> name=NC_012967:2189007..2190527 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2128274..2129794
<gbrowseImage> name=NC_012759:2128274..2129794 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2242623..2241103
<gbrowseImage> name=NC_007779:2241103..2242623 source=W3110 preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmglA (Keio:JW2136)

deletion

deletion

PMID:16738554

Shigen
CGSC9731[5]

mglA::Tn5KAN-2 (FB20651)

Insertion at nt 123 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20651

does not contain pKD46

mglA::Tn5KAN-2 (FB20652)

Insertion at nt 123 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20652

contains pKD46

mgl-51

CGSC:4507

mgl-50

CGSC:5278

mgl-2

CGSC:9752

mgl-500::Tn10

CGSC:15615

mgl-15

CGSC:19380

ΔmglA776::kan

PMID:16738554

CGSC:101848


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2136

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGTCAGCTCAACGACTCCGTC

Primer 2:CCAAGGTGCAAAGACGCAAGACG

7F1

Kohara Phage

Genobase

PMID:3038334

7H12

Kohara Phage

Genobase

PMID:3038334

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[5]

est. P1 cotransduction: 5% [6]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[5]

est. P1 cotransduction: 3% [6]
Synonyms:zeg-722, zei-722::Tn10

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10592

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10592

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000585

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949036

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0587

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007103

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



Product(s)

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MglA

Synonyms

fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components[1], B2149[2][1], MglP[2][1], Mgl[2][1], MglA[2][1] , ECK2142, JW2136, mglP, b2149

Product description

MglA[2][3];

Component of galactose ABC transporter[2][3]

Methyl-galactoside transporter[4]

EC number (for enzymes)


<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of galactose ABC transporter

could be indirect

Protein

mutM

PMID:16606699

Experiment(s):EBI-1142018

Protein

groL

PMID:16606699

Experiment(s):EBI-1142018

Protein

yifB

PMID:16606699

Experiment(s):EBI-1142018

Protein

degP

PMID:16606699

Experiment(s):EBI-1142018

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MVSSTTPSSG EYLLEMSGIN KSFPGVKALD NVNLKVRPHS IHALMGENGA GKSTLLKCLF
GIYQKDSGTI LFQGKEIDFH SAKEALENGI SMVHQELNLV LQRSVMDNMW LGRYPTKGMF
VDQDKMYRET KAIFDELDID IDPRARVGTL SVSQMQMIEI AKAFSYNAKI VIMDEPTSSL
TEKEVNHLFT IIRKLKERGC GIVYISHKME EIFQLCDEVT VLRDGQWIAT EPLAGLTMDK
IIAMMVGRSL NQRFPDKENK PGEVILEVRN LTSLRQPSIR DVSFDLHKGE ILGIAGLVGA
KRTDIVETLF GIREKSAGTI TLHGKQINNH NANEAINHGF ALVTEERRST GIYAYLDIGF
NSLISNIRNY KNKVGLLDNS RMKSDTQWVI DSMRVKTPGH RTQIGSLSGG NQQKVIIGRW
LLTQPEILML DEPTRGIDVG AKFEIYQLIA ELAKKGKGII IISSEMPELL GITDRILVMS
NGLVSGIVDT KTTTQNEILR LASLHL
Length

506

Mol. Wt

56.417 kDa

pI

7.7 (calculated)

Extinction coefficient

36,900 - 37,275 (calc based on 10 Y, 4 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

53..178

PF00005 ABC transporter

PMID:19920124

Domain

303..435

PF00005 ABC transporter

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mglA taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130087

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949036

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007103

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AAG8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10592

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10592

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949036

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000585

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0587

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]




Expression

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

9.79E+01

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

52

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

83

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

21a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

[back to top]


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mglAC

mglBAC

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


<protect><gbrowseImage> name=NC_000913:2237291..2237331 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2149 (EcoliWiki Page)

NCBI GEO profiles for mglA

microarray

GenExpDB:b2149 (EcoliWiki Page)

Summary of data for mglA from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2237226..2237446) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ14; Well:C12[7]

<protect></protect>

Notes

Accessions Related to mglA Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10592

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0587

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2149

EcoGene

EcoGene:EG10592

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000585

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007103

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



Evolution

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000029065 (score: 1.000; bootstrap: 100%)
  • YP_026286 (score: 0.089)

From Inparanoid:20070104

Drosophila pseudoobscura

  • YP_026286 (score: 0.230)
  • GA14368-PA (score: 1.000; bootstrap: 87%)
  • GA13367-PA (score: 0.116)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000027009 (score: 1.000; bootstrap: 92%)

From Inparanoid:20070104

Shigella flexneri

MGLA

From SHIGELLACYC

E. coli O157

MGLA

From ECOO157CYC


Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00005 ABC transporter

Pfam (EcoliWiki Page)

PF00005 ABC transporter

Panther (EcoliWiki Page)

PTHR24220:SF114

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

EcoCyc

EcoCyc:EG10592

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10592

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000585

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0587

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007103

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

[back to top]