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Gene |

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Expression|

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Standard Name	metQ
Gene Synonym(s)	ECK0197, b0197, JW0193, yaeC, metD^[1], metD
Product Desc.	MetQ^[2]^[3]; Component of L- and D-methionine uptake ABC permease^[2]^[3] L,D-methionine transporter, methionine-binding receptor; lipoprotein; methionine sulfoximine sensitivity^[4]
Product Synonyms(s)	DL-methionine transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B0197^[2]^[1], MetD^[2]^[1], MetQ^[2]^[1], YaeC^[2]^[1] , ECK0197, JW0193, metD, yaeC, b0197
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): metNIQ^[2], abc
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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AFMB Structural Genomics target No. 15 (http://afmb.cnrs-mrs.fr/article171.html). pal NlpA paralog. First 22 aa are a type II signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	metQ
Mnemonic	Methionine
Synonyms	ECK0197, b0197, JW0193, yaeC, metD^[1], metD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	4.74 minutes	MG1655: 220928..220113 <gbrowseImage> name=NC_000913:220113..220928 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 223768..222953 <gbrowseImage> name=NC_012967:222953..223768 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 220112..220927 <gbrowseImage> name=NC_012759:220112..220927 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 220928..220113 <gbrowseImage> name=NC_007779:220113..220928 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 195032..194217 <gbrowseImage> name=NC_010473:194217..195032 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
metQ(del) (Keio:JW0193)	deletion	deletion			PMID:16738554	Shigen CGSC8437^[5]
yaeC(del)::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
metQ559::FLKP2(lacZ,kan,Plac)					PMID:12218041	CGSC:87297
metQ722(del)::kan					PMID:16738554	CGSC:101844
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0193	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCGTTCAAATTCAAAACCTT Primer 2:CCCCAGCCTTTAACAGCTCCGCC
10F9	Kohara Phage	Genobase	PMID:3038334
8G3	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 1% ^[6]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 66% ^[6] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11504	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11504	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944893	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001460	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1467	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000666	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MetQ
Synonyms	DL-methionine transporter subunit^[1], periplasmic-binding component of ABC superfamily^[1], B0197^[2]^[1], MetD^[2]^[1], MetQ^[2]^[1], YaeC^[2]^[1] , ECK0197, JW0193, metD, yaeC, b0197
Product description	MetQ^[2]^[3]; Component of L- and D-methionine uptake ABC permease^[2]^[3] L,D-methionine transporter, methionine-binding receptor; lipoprotein; methionine sulfoximine sensitivity^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006865	amino acid transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0029	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of L- and D-methionine uptake ABC permease	could be indirect
Protein	araG		PMID:15690043	Experiment(s):EBI-891576
Protein	yfaY		PMID:15690043	Experiment(s):EBI-891576
Protein	tufA		PMID:15690043	Experiment(s):EBI-891576
Protein	kefF		PMID:15690043	Experiment(s):EBI-891576
Protein	yahK		PMID:15690043	Experiment(s):EBI-891576
Protein	gfcA		PMID:15690043	Experiment(s):EBI-891576
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1135924
Protein	yfaY		PMID:19402753	LCMS(ID Probability):99.6
Protein	araG		PMID:19402753	LCMS(ID Probability):99.6
Protein	yahK		PMID:19402753	LCMS(ID Probability):99.6
Protein	kefF		PMID:19402753	LCMS(ID Probability):99.6
Protein	gfcA		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Inner membrane	PMID:12218041	EchoLocation:metQ
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAFKFKTFAA VGALIGSLAL VGCGQDEKDP NHIKVGVIVG AEQQVAEVAQ KVAKDKYGLD VELVTFNDYV LPNEALSKGD IDANAFQHKP YLDQQLKDRG YKLVAVGNTF VYPIAGYSKK IKSLDELQDG SQVAVPNDPT NLGRSLLLLQ KVGLIKLKDG VGLLPTVLDV VENPKNLKIV ELEAPQLPRS LDDAQIALAV INTTYASQIG LTPAKDGIFV EDKESPYVNL IVTREDNKDA ENVKKFVQAY QSDEVYEAAN KVFNGGAVKG W
Length	271
Mol. Wt	29.431 kDa
pI	5.0 (calculated)
Extinction coefficient	20,400 - 20,525 (calc based on 10 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-22	UniProt Manual:Signal Peptides	UniProt:P28635
Domain	33..271	PF03180 NLPA lipoprotein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=metQ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128190	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944893	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000666	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P28635	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11504	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11504	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944893	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001460	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1467	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	8.05E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	15.557+/-0.117	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	7841	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	10568	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	25871	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
metNIQ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:220908..220948 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0197 (EcoliWiki Page)	NCBI GEO profiles for metQ
microarray	GenExpDB:b0197 (EcoliWiki Page)	Summary of data for metQ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to metQ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11504	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1467	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0197
EcoGene	EcoGene:EG11504	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001460	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000666	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YAEC	From SHIGELLACYC
E. coli O157	YAEC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03180 NLPA lipoprotein
Superfamily (EcoliWiki Page)	SUPERFAMILY:53850
EcoCyc	EcoCyc:EG11504	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11504	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001460	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1467	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000666	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

metQ:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to metQ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

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