malT:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

malT

Gene Synonym(s)

ECK3405, b3418, JW3381, malA[1], malA

Product Desc.

MalT transcriptional activator[2][3]

ATP-dependent transcriptional activator for the mal regulon[4]

Product Synonyms(s)

fused conserved protein[1], DNA-binding transcriptional activator, maltotriose-ATP-binding[1], MalA[2][1], B3418[2][1] , ECK3405, JW3381, malA, b3418

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): malT[2], OP00128

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Activated by maltotriose and ATP to form active oligomers. Signal transduction ATPases with numerous domains (STAND) protein. ATP hydrolysis is not required for transcription activation (Richet, 1989), but is required to recycle back to the inactive form, which has ADP, not ATP bound (Marquenet, 2007). MalY (Clausen, 2000; Schreiber, 2000), MalK (Reyes, 1988; Panagiotidis, 1998; Bohm, 2002) and Aes (Joly, 2002) negatively regulate MalT activity by binding and blocking maltotriose activation. MalT is required for phage lambda sensitivity.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

malT

Mnemonic

Maltose

Synonyms

ECK3405, b3418, JW3381, malA[1], malA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.54 minutes 

MG1655: 3551107..3553812
<gbrowseImage> name=NC_000913:3551107..3553812 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3481685..3484390
<gbrowseImage> name=NC_012967:3481685..3484390 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3439601..3442306
<gbrowseImage> name=NC_012759:3439601..3442306 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4087331..4084626
<gbrowseImage> name=NC_007779:4084626..4087331 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3648852..3651557
<gbrowseImage> name=NC_010473:3648852..3651557 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmalT (Keio:JW3381)

deletion

deletion

PMID:16738554

Shigen
CGSC10518[5]

malT::Tn5KAN-I-SceI (FB21188)

Insertion at nt 1497 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21188

does not contain pKD46

malT::Tn5KAN-I-SceI (FB21189)

Insertion at nt 1497 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21189

contains pKD46

malT1(LamR)

lambda resistant

CGSC:4749

malT36(LamR)

lambda resistant

CGSC:5640

malT352(LamR)

lambda resistant

CGSC:8216

malT354(LamR)

lambda resistant

CGSC:8222

malT101(Const)

constitutive

PMID:6094487

CGSC:9076

malT54::Tn10

CGSC:9950

malT56::Tn5

CGSC:9954

malT66(Am, LamR)

lambda resistant

CGSC:10568

amber (UAG) mutation

ΔmalT752::kan

PMID:16738554

CGSC:101746

malT in strain POP1002

Resistant to

lambda phage Resistance

PMID:4558324

Mutant Strain: POP1002


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3381

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCTGATTCCGTCAAAACTAAG

Primer 2:CCaACGCCGTACCCCATCATCTT

E4E4

Kohara Phage

Genobase

PMID:3038334

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 11% [6]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[5]

est. P1 cotransduction: 66% [6]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10562

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10562

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000555

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947921

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0557

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011162

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MalT

Synonyms

fused conserved protein[1], DNA-binding transcriptional activator, maltotriose-ATP-binding[1], MalA[2][1], B3418[2][1] , ECK3405, JW3381, malA, b3418

Product description

MalT transcriptional activator[2][3]

ATP-dependent transcriptional activator for the mal regulon[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000156

two-component response regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016032

F

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016032

P

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016032

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01247

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000792

F

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011990

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01247

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000792

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016032

C

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0119

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000792

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016032

P

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000792

F

Seeded from EcoCyc (v14.0)

complete

GO:0045893

positive regulation of transcription, DNA-dependent

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01247

P

Seeded from EcoCyc (v14.0)

complete

GO:0045913

positive regulation of carbohydrate metabolic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01247

P

Seeded from EcoCyc (v14.0)

complete

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Interactions


See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

fixB

PMID:15690043

Experiment(s):EBI-879887

Protein

fliJ

PMID:15690043

Experiment(s):EBI-879887

Protein

metK

PMID:15690043

Experiment(s):EBI-879887

Protein

groL

PMID:15690043

Experiment(s):EBI-879887

Protein

pstB

PMID:15690043

Experiment(s):EBI-879887

Protein

rho

PMID:15690043

Experiment(s):EBI-879887

Protein

rplS

PMID:15690043

Experiment(s):EBI-879887

Protein

tufA

PMID:15690043

Experiment(s):EBI-879887

Protein

tufB

PMID:19402753

MALDI(Z-score):19.113942

Protein

pstB

PMID:19402753

MALDI(Z-score):23.266028

Protein

dnaJ

PMID:19402753

MALDI(Z-score):31.533384

Protein

mreB

PMID:19402753

MALDI(Z-score):19.231416

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLIPSKLSRP VRLDHTVVRE RLLAKLSGAN NFRLALITSP AGYGKTTLIS QWAAGKNDIG
WYSLDEGDNQ QERFASYLIA AVQQATNGHC AICETMAQKR QYASLTSLFA QLFIELAEWH
SPLYLVIDDY HLITNPVIHE SMRFFIRHQP ENLTLVVLSR NLPQLGIANL RVRDQLLEIG
SQQLAFTHQE AKQFFDCRLS SPIEAAESSR ICDDVSGWAT ALQLIALSAR QNTHSAHKSA
RRLAGINASH LSDYLVDEVL DNVDLATRHF LLKSAILRSM NDALITRVTG EENGQMRLEE
IERQGLFLQR MDDTGEWFCY HPLFGNFLRQ RCQWELAAEL PEIHRAAAES WMAQGFPSEA
IHHALAAGDA LMLRDILLNH AWSLFNHSEL SLLEESLKAL PWDSLLENPQ LVLLQAWLMQ
SQHRYGEVNT LLARAEHEIK DIREDTMHAE FNALRAQVAI NDGNPDEAER LAKLALEELP
PGWFYSRIVA TSVLGEVLHC KGELTRSLAL MQQTEQMARQ HDVWHYALWS LIQQSEILFA
QGFLQTAWET QEKAFQLINE QHLEQLPMHE FLVRIRAQLL WAWARLDEAE ASARSGIEVL
SSYQPQQQLQ CLAMLIQCSL ARGDLDNARS QLNRLENLLG NGKYHSDWIS NANKVRVIYW
QMTGDKAAAA NWLRHTAKPE FANNHFLQGQ WRNIARAQIL LGEFEPAEIV LEELNENARS
LRLMSDLNRN LLLLNQLYWQ AGRKSDAQRV LLDALKLANR TGFISHFVIE GEAMAQQLRQ
LIQLNTLPEL EQHRAQRILR EINQHHRHKF AHFDENFVER LLNHPEVPEL IRTSPLTQRE
WQVLGLIYSG YSNEQIAGEL EVAATTIKTH IRNLYQKLGV AHRQDAVQHA QQLLKMMGYG V
Length

901

Mol. Wt

103.12 kDa

pI

6.5 (calculated)

Extinction coefficient

149,310 - 150,435 (calc based on 19 Y, 22 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

833..890

PF00196 Bacterial regulatory proteins, luxR family

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=malT taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131294

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947921

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011162

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P06993

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10562

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10562

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947921

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000555

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0557

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

377

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

299

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

213

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

malT

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3551087..3551127 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3418 (EcoliWiki Page)

NCBI GEO profiles for malT

microarray

GenExpDB:b3418 (EcoliWiki Page)

Summary of data for malT from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to malT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10562

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0557

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3418

EcoGene

EcoGene:EG10562

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000555

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011162

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments
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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00196 Bacterial regulatory proteins, luxR family

Superfamily (EcoliWiki Page)

SUPERFAMILY:46894

Superfamily (EcoliWiki Page)

SUPERFAMILY:48452

EcoCyc

EcoCyc:EG10562

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10562

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000555

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0557

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011162

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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