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Standard Name	lpxL
Gene Synonym(s)	ECK1039, b1054, JW1041, htrB, waaM^[1], waaM
Product Desc.	Lipid A biosynthesis, KDO2-lipid IVA lauroyl-ACP acyltransferase; not under heat shock regulation; membrane protein affecting cell division, growth, and high-temperature survival^[2]
Product Synonyms(s)	lauryl-acyl carrier protein (ACP)-dependent acyltransferase^[1], B1054^[3]^[1], WaaM^[3]^[1], HtrB^[3]^[1], LpxL^[3]^[1] , ECK1039, htrB, JW1041, waaM, b1054
Function from GO	<GO_nr />
Knock-Out Phenotype	Does not affect growth at low temperatures but causes a loss of viability at high temperatures in rich media (see PMID:1358874)
Regulation/Expression	transcription unit(s): lpxL^[3]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lpxL
Mnemonic	Lipid A expression/biosynthesis
Synonyms	ECK1039, b1054, JW1041, htrB, waaM^[1], waaM
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	24.03 minutes	MG1655: 1115805..1114885 <gbrowseImage> name=NC_000913:1114885..1115805 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1018810..1019730 <gbrowseImage> name=NC_012759:1018810..1019730 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1118159..1117239 <gbrowseImage> name=NC_007779:1117239..1118159 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1171250..1170330 <gbrowseImage> name=NC_010473:1170330..1171250 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability
ΔlpxL (Keio:JW1041)	deletion	deletion		PMID:16738554	Shigen
lpxL10(ts)::mini-Tn10			temperature sensitive	PMID:1656498 PMID:1846149	CGSC:65138
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1041	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACGAATCTACCCAAGTTCTC Primer 2:CCATAGCGTGAAGGAACGCCTTC
1H7	Kohara Phage	Genobase	PMID:3038334
E4H10S	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[4]	est. P1 cotransduction: 8% ^[5]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[4]	est. P1 cotransduction: 18% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10464	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10464	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000457	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946216	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0459	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003574	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LpxL
Synonyms	lauryl-acyl carrier protein (ACP)-dependent acyltransferase^[1], B1054^[3]^[1], WaaM^[3]^[1], HtrB^[3]^[1], LpxL^[3]^[1] , ECK1039, htrB, JW1041, waaM, b1054
Product description	Lipid A biosynthesis, KDO2-lipid IVA lauroyl-ACP acyltransferase; not under heat shock regulation; membrane protein affecting cell division, growth, and high-temperature survival^[2]
EC number (for enzymes)	2.3.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006950	response to stress	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008415	acyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004960	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008415	acyltransferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0012	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009244	lipopolysaccharide core region biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004960	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009276	Gram-negative-bacterium-type cell wall	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011920	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004960	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	PMID:8662613	IDA: Inferred from Direct Assay		F		complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011920	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:8662613	IDA: Inferred from Direct Assay		C		complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009245	lipid A biosynthetic process	PMID:11830595	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9904	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016747	transferase activity, transferring acyl groups other than amino-acyl groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011920	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1138705
Protein	rplB	PMID:16606699	Experiment(s):EBI-1138705
Protein	groL	PMID:16606699	Experiment(s):EBI-1138705
Protein	ycgE	PMID:16606699	Experiment(s):EBI-1138705
Protein	ompL	PMID:16606699	Experiment(s):EBI-1138705
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1138705
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[6]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTNLPKFSTA LLHPRYWLTW LGIGVLWLVV QLPYPVIYRL GCGLGKLALR FMKRRAKIVH RNLELCFPEM SEQERRKMVV KNFESVGMGL METGMAWFWP DRRIARWTEV IGMEHIRDVQ AQKRGILLVG IHFLTLELGA RQFGMQEPGI GVYRPNDNPL IDWLQTWGRL RSNKSMLDRK DLKGMIKALK KGEVVWYAPD HDYGPRSSVF VPLFAVEQAA TTTGTWMLAR MSGACLVPFV PRRKPDGKGY QLIMLPPECS PPLDDAETTA AWMNKVVEKC IMMAPEQYMW LHRRFKTRPE GVPSRY
Length	306
Mol. Wt	35.407 kDa
pI	10.1 (calculated)
Extinction coefficient	79,410 - 80,035 (calc based on 9 Y, 12 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..297	PF03279 Bacterial lipid A biosynthesis acyltransferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lpxL taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129017	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946216	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003574	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACV0	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10464	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10464	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946216	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000457	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0459	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	938	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	168	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	446	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
lpxL
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1115785..1115825 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1054 (EcoliWiki Page)	NCBI GEO profiles for lpxL
microarray	GenExpDB:b1054 (EcoliWiki Page)	Summary of data for lpxL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to lpxL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10464	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0459	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1054
EcoGene	EcoGene:EG10464	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000457	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003574	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000029017 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	HTRB	From SHIGELLACYC
E. coli O157	Z1690	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03279 Bacterial lipid A biosynthesis acyltransferase
EcoCyc	EcoCyc:EG10464	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10464	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000457	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0459	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003574	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

lpxL:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lpxL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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