lpxL:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lpxL

Mnemonic

Lipid A expression/biosynthesis

Synonyms

ECK1039, b1054, JW1041, htrB, waaM[1], waaM

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

24.03 minutes 

MG1655: 1115805..1114885
<gbrowseImage> name=NC_000913:1114885..1115805 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1018810..1019730
<gbrowseImage> name=NC_012759:1018810..1019730 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1118159..1117239
<gbrowseImage> name=NC_007779:1117239..1118159 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1171250..1170330
<gbrowseImage> name=NC_010473:1170330..1171250 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔlpxL (Keio:JW1041)

deletion

deletion

PMID:16738554[2]

Shigen

lpxL10(ts)::mini-Tn10

temperature sensitive

PMID:1656498[3] PMID:1846149[4]

CGSC:65138


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1041

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACGAATCTACCCAAGTTCTC

Primer 2:CCATAGCGTGAAGGAACGCCTTC

1H7

Kohara Phage

Genobase

PMID:3038334[6]

E4H10S

Kohara Phage

Genobase

PMID:3038334[6]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[7]

est. P1 cotransduction: 8% [8]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[7]

est. P1 cotransduction: 18% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10464

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10464

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000457

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946216

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0459

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003574

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. Karow, M et al. () Complex phenotypes of null mutations in the htr genes, whose products are essential for Escherichia coli growth at elevated temperatures. Res. Microbiol. 142 289-94 PubMed
  4. Karow, M et al. (1991) Isolation and characterization of the Escherichia coli htrB gene, whose product is essential for bacterial viability above 33 degrees C in rich media. J. Bacteriol. 173 741-50 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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