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Product(s) |

Expression|

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Standard Name	lolB
Gene Synonym(s)	ECK1197, b1209, JW1200, hemM, ychC^[1], ychC
Product Desc.	outer membrane lipoprotein, localization of lipoproteins in the outer membrane^[2]^[3] OM lipoprotein required for localization of lipoproteins; essential gene^[4]
Product Synonyms(s)	chaperone for lipoproteins^[1], B1209^[2]^[1], YchC^[2]^[1], LolB^[2]^[1], HemM^[2]^[1] , ECK1197, hemM, JW1200, ychC, b1209
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): lolB-ispE-prsA^[2], lolB-ispE-prs
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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First 21 aa are a type II signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lolB
Mnemonic	Localization OM lipoproteins
Synonyms	ECK1197, b1209, JW1200, hemM, ychC^[1], ychC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	27.2 minutes	MG1655: 1262723..1262100 <gbrowseImage> name=NC_000913:1262100..1262723 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1263650..1263027 <gbrowseImage> name=NC_012967:1263027..1263650 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1150821..1151444 <gbrowseImage> name=NC_012759:1150821..1151444 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1265077..1264454 <gbrowseImage> name=NC_007779:1264454..1265077 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1302964..1302341 <gbrowseImage> name=NC_010473:1302341..1302964 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1200	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCCCTGCCCGATTTTCGTCT Primer 2:CCTTTCACTATCCAGTTATCCAT
12A3	Kohara Phage	Genobase	PMID:3038334
4D10	Kohara Phage	Genobase	PMID:3038334
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 34% ^[6]
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11293	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11293	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001266	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945775	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1270	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004059	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LolB
Synonyms	chaperone for lipoproteins^[1], B1209^[2]^[1], YchC^[2]^[1], LolB^[2]^[1], HemM^[2]^[1] , ECK1197, hemM, JW1200, ychC, b1209
Product description	outer membrane lipoprotein, localization of lipoproteins in the outer membrane^[2]^[3] OM lipoprotein required for localization of lipoproteins; essential gene^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008565	protein transporter activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00233	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004565	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	PMID:9384574	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004565	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0653	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030228	lipoprotein receptor activity	PMID:9384574	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	ribB	PMID:16606699	Experiment(s):EBI-1139180
Protein	napC	PMID:16606699	Experiment(s):EBI-1139180
Protein	ansB	PMID:16606699	Experiment(s):EBI-1139180
Protein	asnA	PMID:16606699	Experiment(s):EBI-1139180
Protein	hisJ	PMID:16606699	Experiment(s):EBI-1139180
Protein	slyD	PMID:19402753	LCMS(ID Probability):99.0 MALDI(Z-score):2.517588
Protein	rpsA	PMID:19402753	MALDI(Z-score):20.143679
Protein	rho	PMID:19402753	MALDI(Z-score):28.140727
Protein	rfaD	PMID:19402753	LCMS(ID Probability):99.0 MALDI(Z-score):39.441705
Protein	murF	PMID:19402753	LCMS(ID Probability):99.0
Protein	pflB	PMID:19402753	MALDI(Z-score):21.295298
Protein	rpsC	PMID:19402753	LCMS(ID Probability):99.0 MALDI(Z-score):5.287256
Protein	srmB	PMID:19402753	MALDI(Z-score):28.163542
Protein	hyaB	PMID:19402753	MALDI(Z-score):28.299865
Protein	secB	PMID:19402753	LCMS(ID Probability):99.0
Protein	lon	PMID:19402753	MALDI(Z-score):27.429843
Protein	ppiD	PMID:19402753	LCMS(ID Probability):99.0
Protein	ftnA	PMID:19402753	LCMS(ID Probability):99.0
Protein	yrfF	PMID:19402753	LCMS(ID Probability):99.0
Protein	ychN	PMID:19402753	LCMS(ID Probability):99.0
Protein	betB	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
outer membrane
Outer membrane	PMID:16091355	EchoLocation:lolB
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPLPDFRLIR LLPLAALVLT ACSVTTPKGP GKSPDSPQWR QHQQDVRNLN QYQTRGAFAY ISDQQKVYAR FFWQQTGQDR YRLLLTNPLG STELELNAQP GNVQLVDNKG QRYTADDAEE MIGKLTGMPI PLNSLRQWIL GLPGDATDYK LDDQYRLSEI TYSQNGKNWK VVYGGYDTKT QPAMPANMEL TDGGQRIKLK MDNWIVK
Length	207
Mol. Wt	23.551 kDa
pI	9.0 (calculated)
Extinction coefficient	42,400 - 42,525 (calc based on 10 Y, 5 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-21	UniProt Manual:Signal Peptides	UniProt:P61320
Domain	48..204	PF03550 Outer membrane lipoprotein LolB		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lolB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129172	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945775	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004059	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P61320	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11293	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11293	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945775	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001266	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1270	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.23E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1417	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	782	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	610	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
lolB-ispE-prsA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1262703..1262743 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1209 (EcoliWiki Page)	NCBI GEO profiles for lolB
microarray	GenExpDB:b1209 (EcoliWiki Page)	Summary of data for lolB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1262653..1262995) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:G12^[7]
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Notes

Accessions Related to lolB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11293	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1270	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1209
EcoGene	EcoGene:EG11293	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001266	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004059	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	HEMM	From SHIGELLACYC
E. coli O157	HEMM	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03550 Outer membrane lipoprotein LolB
Superfamily (EcoliWiki Page)	SUPERFAMILY:89392
EcoCyc	EcoCyc:EG11293	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11293	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001266	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1270	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004059	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

lolB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lolB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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