lepB:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
lepB |
---|---|
Gene Synonym(s) |
ECK2566, b2568, JW2552, lep[1], lep |
Product Desc. |
leader peptidase (signal peptidase I)[2][3] Signal peptidase I; SPI; responsible for type I signal cleavages of periplasmic, OM, some IM, and extracellular proteins[4] |
Product Synonyms(s) |
leader peptidase (signal peptidase I)[1], B2568[2][1], Lep[2][1], LepB[2][1], LP1[2][1] , ECK2566, JW2552, lep, b2568 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
LepB has a blocked N-terminus. False positive lipoprotein prediction, shown to have no signal peptide. Targetted by SRP/FtsY to Sec/YidC for membrane insertion.[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
lepB |
---|---|
Mnemonic |
Leader peptidase |
Synonyms |
ECK2566, b2568, JW2552, lep[1], lep |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
58.24 minutes |
MG1655: 2703331..2702357 |
||
NC_012967: 2626802..2625828 |
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NC_012759: 2588162..2589136 |
||||
W3110 |
|
W3110: 2703965..2702991 |
||
DH10B: 2795096..2794122 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
lepBC171A |
C171A |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBK146M,D,G,S |
K146M,D,G,S |
Loss of activity |
seeded from UniProt:P00803 | ||||
lepBR147Q |
R147Q |
Small effect |
seeded from UniProt:P00803 | ||||
lepBD154A |
D154A |
Loss of activity |
seeded from UniProt:P00803 | ||||
lepBD154N |
D154N |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBE62V |
E62V |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBR78E,N,L |
R78E,N,L |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBS91A |
S91A |
Loss of activity |
seeded from UniProt:P00803 | ||||
lepBH125N |
H125N |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBR128Q |
R128Q |
Small effect |
seeded from UniProt:P00803 | ||||
lepBD130A |
D130A |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBY144F |
Y144F |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBD159N |
D159N |
Small effect |
seeded from UniProt:P00803 | ||||
lepBC177A |
C177A |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBH236N |
H236N |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBY269F |
Y269F |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBD274A |
D274A |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBR276Q |
R276Q |
Small effect |
seeded from UniProt:P00803 | ||||
lepBD281A |
D281A |
Indifferent |
seeded from UniProt:P00803 | ||||
lepBR283Q |
R283Q |
Small effect |
seeded from UniProt:P00803 | ||||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW2552 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGCGAATATGTTTGCCCTGAT Primer 2:CCATGGATGCCGCCAATGCGACT | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 19% [6] | ||
Linked marker |
est. P1 cotransduction: 78% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000523 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0525 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0008450 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
LepB |
---|---|
Synonyms |
leader peptidase (signal peptidase I)[1], B2568[2][1], Lep[2][1], LepB[2][1], LP1[2][1] , ECK2566, JW2552, lep, b2568 |
Product description |
leader peptidase (signal peptidase I)[2][3] Signal peptidase I; SPI; responsible for type I signal cleavages of periplasmic, OM, some IM, and extracellular proteins[4] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006508 |
proteolysis |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000223 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008233 |
peptidase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0645 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008236 |
serine-type peptidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000223 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008236 |
serine-type peptidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019756 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008236 |
serine-type peptidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019757 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016485 |
protein processing |
PMID:11511369 |
IMP: Inferred from Mutant Phenotype |
UniProtKB:P17998 |
P |
LepB is required for cleavage and subsequent activation of Colicin D. |
complete | |
GO:0008236 |
serine-type peptidase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019758 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0015643 |
toxin binding |
PMID:11511369 |
IMP: Inferred from Mutant Phenotype |
UniProtKB:P17998 |
F |
LepB binds and cleaves Colicin D, which activates the colicin. |
complete | |
GO:0015643 |
toxin binding |
PMID:11511369 |
IMP: Inferred from Mutant Phenotype |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0008233 |
peptidase activity |
PMID:15195997 |
IDA: Inferred from Direct Assay |
F |
complete | |||
GO:0015643 |
toxin binding |
PMID:11511369 |
IMP: Inferred from Mutant Phenotype |
UniProtKB:P17998 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006508 |
proteolysis |
PMID:15195997 |
IMP: Inferred from Mutant Phenotype |
P |
complete | |||
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019756 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006508 |
proteolysis |
PMID:10891078 |
IDA: Inferred from Direct Assay |
P |
complete | |||
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019757 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016485 |
protein processing |
PMID:15598653 |
IMP: Inferred from Mutant Phenotype |
P |
complete | |||
GO:0016021 |
integral to membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR019758 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
PMID:10891078 |
IDA: Inferred from Direct Assay |
C |
complete | |||
GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0812 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
PMID:10891078 |
IDA: Inferred from Direct Assay |
C |
complete | |||
GO:0016021 |
integral to membrane |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0008233 |
peptidase activity |
PMID:15598653 |
IMP: Inferred from Mutant Phenotype |
F |
complete | |||
GO:0016485 |
protein processing |
PMID:11511369 |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0016485 |
protein processing |
PMID:11511369 |
IMP: Inferred from Mutant Phenotype |
UniProtKB:P17998 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
plasma membrane |
C-terminus localized to the periplasm with 2 predicted transmembrane domains |
Daley et al. (2005) [7] |
||
plasma membrane |
| |||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MANMFALILV IATLVTGILW CVDKFFFAPK RRERQAAAQA AAGDSLDKAT LKKVAPKPGW LETGASVFPV LAIVLIVRSF IYEPFQIPSG SMMPTLLIGD FILVEKFAYG IKDPIYQKTL IETGHPKRGD IVVFKYPEDP KLDYIKRAVG LPGDKVTYDP VSKELTIQPG CSSGQACENA LPVTYSNVEP SDFVQTFSRR NGGEATSGFF EVPKNETKEN GIRLSERKET LGDVTHRILT VPIAQDQVGM YYQQPGQQLA TWIVPPGQYF MMGDNRDNSA DSRYWGFVPE ANLVGRATAI WMSFDKQEGE WPTGLRLSRI GGIH |
Length |
324 |
Mol. Wt |
35.96 kDa |
pI |
7.4 (calculated) |
Extinction coefficient |
49,390 - 49,765 (calc based on 11 Y, 6 W, and 3 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0008450 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000523 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0525 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MG1655 |
1315 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
338 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
670 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:2703311..2703351
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for lepB | |
microarray |
Summary of data for lepB from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to lepB Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0525 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000523 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0008450 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Anopheles gambiae |
|
From Inparanoid:20070104 |
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Bos taurus |
|
From Inparanoid:20070104 |
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
Canis familiaris |
|
From Inparanoid:20070104 |
Ciona intestinalis |
|
From Inparanoid:20070104 |
Gallus gallus |
|
From Inparanoid:20070104 |
Homo sapiens |
|
From Inparanoid:20070104 |
Mus musculus |
|
From Inparanoid:20070104 |
Pan troglodytes |
|
From Inparanoid:20070104 |
Rattus norvegicus |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
Xenopus tropicalis |
|
From Inparanoid:20070104 |
Shigella flexneri |
LEPB |
From SHIGELLACYC |
E. coli O157 |
LEPB |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10530 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000523 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0525 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0008450 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
Categories
- TMHMM Prediction
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Mus musculus
- Genes with homologs in Pan troglodytes
- Genes with homologs in Rattus norvegicus
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157