lepB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
lepB |
|---|---|
| Mnemonic |
Leader peptidase |
| Synonyms |
ECK2566, b2568, JW2552, lep[1], lep |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
58.24 minutes |
MG1655: 2703331..2702357 |
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NC_012967: 2626802..2625828 |
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NC_012759: 2588162..2589136 |
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W3110 |
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W3110: 2703965..2702991 |
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DH10B: 2795096..2794122 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
lepBC171A |
C171A |
Indifferent |
seeded from UniProt:P00803 | ||||
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lepBK146M,D,G,S |
K146M,D,G,S |
Loss of activity |
seeded from UniProt:P00803 | ||||
|
lepBR147Q |
R147Q |
Small effect |
seeded from UniProt:P00803 | ||||
|
lepBD154A |
D154A |
Loss of activity |
seeded from UniProt:P00803 | ||||
|
lepBD154N |
D154N |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBE62V |
E62V |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBR78E,N,L |
R78E,N,L |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBS91A |
S91A |
Loss of activity |
seeded from UniProt:P00803 | ||||
|
lepBH125N |
H125N |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBR128Q |
R128Q |
Small effect |
seeded from UniProt:P00803 | ||||
|
lepBD130A |
D130A |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBY144F |
Y144F |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBD159N |
D159N |
Small effect |
seeded from UniProt:P00803 | ||||
|
lepBC177A |
C177A |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBH236N |
H236N |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBY269F |
Y269F |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBD274A |
D274A |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBR276Q |
R276Q |
Small effect |
seeded from UniProt:P00803 | ||||
|
lepBD281A |
D281A |
Indifferent |
seeded from UniProt:P00803 | ||||
|
lepBR283Q |
R283Q |
Small effect |
seeded from UniProt:P00803 | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2552 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCGAATATGTTTGCCCTGAT Primer 2:CCATGGATGCCGCCAATGCGACT | |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 19% [5] | ||
|
Linked marker |
est. P1 cotransduction: 78% [5] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 3.0 3.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
