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Standard Name	kefG
Gene Synonym(s)	ECK3339, b3351, JW3314, yheR^[1], yheR
Product Desc.	protein required for KefB activity^[2]^[3] Required for KefB-mediated K+ efflux; member of quinone oxidoreductase family, but lacks key residues needed for oxidoreductase activity^[4]
Product Synonyms(s)	component of potassium effux complex with KefB^[1], B3351^[2]^[1], KefG^[2]^[1], YheR^[2]^[1] , ECK3339, JW3314, yheR, b3351
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yheV^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	kefG
Mnemonic	K+ efflux
Synonyms	ECK3339, b3351, JW3314, yheR^[1], yheR
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	74.98 minutes	MG1655: 3479183..3478629 <gbrowseImage> name=NC_000913:3478629..3479183 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3408512..3409066 <gbrowseImage> name=NC_012967:3408512..3409066 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3365786..3366340 <gbrowseImage> name=NC_012759:3365786..3366340 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4159255..4159809 <gbrowseImage> name=NC_007779:4159255..4159809 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3576928..3576374 <gbrowseImage> name=NC_010473:3576374..3576928 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔkefG (Keio:JW3314)	deletion	deletion	PMID:16738554	Shigen CGSC10476^[5]
ΔkefG769::kan			PMID:16738554	CGSC:101630
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3314	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGTCTCAGCCAGCGAAAGT Primer 2:CCGCGGCCTCCTGGAGACAGCGG
4C5	Kohara Phage	Genobase	PMID:3038334
3F8	Kohara Phage	Genobase	PMID:3038334
smg-3082::Tn10	Linked marker	CAG12071 = CGSC7444^[5]	est. P1 cotransduction: 10% ^[6] Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 40% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7717	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12902	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004262	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947857	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2739	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010951	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	KefG
Synonyms	component of potassium effux complex with KefB^[1], B3351^[2]^[1], KefG^[2]^[1], YheR^[2]^[1] , ECK3339, JW3314, yheR, b3351
Product description	protein required for KefB activity^[2]^[3] Required for KefB-mediated K+ efflux; member of quinone oxidoreductase family, but lacks key residues needed for oxidoreductase activity^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006813	potassium ion transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01415	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009055	electron carrier activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050662	coenzyme binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003680	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	ydaL	PMID:16606699	Experiment(s):EBI-1145657
Protein	ydfE	PMID:16606699	Experiment(s):EBI-1145657
Protein	ompR	PMID:16606699	Experiment(s):EBI-1145657
Protein	ribB	PMID:16606699	Experiment(s):EBI-1145657
Protein	groL	PMID:16606699	Experiment(s):EBI-1145657
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMSQPAKVLL LYAHPESQDS VANRVLLKPA TQLSNVTVHD LYAHYPDFFI DIPREQALLR EHEVIVFQHP LYTYSCPALL KEWLDRVLSR GFASGPGGNQ LAGKYWRSVI TTGEPESAYR YDALNRYPMS DVLRPFELAA GMCRMHWLSP IIIYWARRQS AQELASHARA YGDWLANPLS PGGR
Length	184
Mol. Wt	20.958 kDa
pI	8.1 (calculated)
Extinction coefficient	43,890 - 44,140 (calc based on 11 Y, 5 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	6..175	PF02525 Flavodoxin-like fold		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=kefG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131230	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947857	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010951	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A756	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7717	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12902	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947857	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004262	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2739	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	69	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	66	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	56^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yheV
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3479163..3479203 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3351 (EcoliWiki Page)	NCBI GEO profiles for kefG
microarray	GenExpDB:b3351 (EcoliWiki Page)	Summary of data for kefG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3478721..3479007) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ14; Well:H2^[7]
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Notes

Accessions Related to kefG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7717	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2739	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3351
EcoGene	EcoGene:EG12902	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004262	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010951	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000402 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000034446 (score: 1.000; bootstrap: 100%) ENSAPMP00000022018 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000039940 (score: 1.000; bootstrap: 100%) ENSBTAP00000032251 (score: 0.410)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000013713 (score: 1.000; bootstrap: 100%) ENSCAFP00000030044 (score: 0.362)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-030131-1226 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-1145 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000020892 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000337773 (score: 1.000; bootstrap: 100%) ENSP00000319788 (score: 0.359)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000011126 (score: 1.000; bootstrap: 100%) ENSMMUP00000025865 (score: 0.360)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000003283 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:103187 (score: 1.000; bootstrap: 100%) MGI:104513 (score: 0.320)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000030178 (score: 1.000; bootstrap: 100%) ENSPTRP00000014148 (score: 0.361)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000017175 (score: 1.000; bootstrap: 100%) ENSRNOP00000024141 (score: 0.338) ENSRNOP00000033201 (score: 0.303)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000145774 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YHER	From SHIGELLACYC
E. coli O157	YHER	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02525 Flavodoxin-like fold
Superfamily (EcoliWiki Page)	SUPERFAMILY:52218
EcoCyc	EcoCyc:G7717	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12902	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004262	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2739	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010951	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

kefG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to kefG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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