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Standard Name	kbaZ
Gene Synonym(s)	ECK3120, b3132, JW3101, agaZ, yhaX^[1], yhaX
Product Desc.	G7631^[2]^[3]; Component of tagatose-1,6-bisphosphate aldolase 1^[3] Ketose bisphosphate aldolase KbaYZ subunit; required for full activity and stabilty of KbaY^[4]
Product Synonyms(s)	tagatose 6-phosphate aldolase 1, kbaZ subunit^[1], B3132^[2]^[1], YhaX^[2]^[1], KbaZ^[2]^[1], AgaZ^[2]^[1] , agaZ, ECK3120, JW3101, yhaX, b3132
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): kbaZ-agaVWA^[2], kbaZagaVWEFA, agaZVWA
Regulation/Activity
Quick Links
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Notes

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Part of aga cluster.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	kbaZ
Mnemonic	Ketose bisphosphate aldolase
Synonyms	ECK3120, b3132, JW3101, agaZ, yhaX^[1], yhaX
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	70.63 minutes	MG1655: 3276936..3278216 <gbrowseImage> name=NC_000913:3276936..3278216 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3211842..3213122 <gbrowseImage> name=NC_012967:3211842..3213122 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3164084..3165364 <gbrowseImage> name=NC_012759:3164084..3165364 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3278769..3280049 <gbrowseImage> name=NC_007779:3278769..3280049 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3374681..3375961 <gbrowseImage> name=NC_010473:3374681..3375961 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔkbaZ (Keio:JW3101)	deletion	deletion	PMID:16738554	Shigen
agaZ::Tn5KAN-I-SceI (FB21032)	Insertion at nt 745 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21032	does not contain pKD46
agaZ::Tn5KAN-I-SceI (FB21033)	Insertion at nt 745 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21033	contains pKD46
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3101	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACATCTGACAGAAATGGT Primer 2:CCTTGGCCTTCACAGGCTGTGTG
20F4	Kohara Phage	Genobase	PMID:3038334
aer-3075::Tn10	Linked marker	CAG12152 = CGSC7438^[5]	est. P1 cotransduction: 4% ^[6] Synonyms:zgh-3075::Tn10, zgj-3075::Tn10
sfsB203::Tn10	Linked marker	CAG12072 = CGSC7440^[5]	est. P1 cotransduction: 7% ^[6] Synonyms:zgj-203::Tn10, zha-203::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7631	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12763	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004181	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947637	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2616	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010295	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	KbaZ
Synonyms	tagatose 6-phosphate aldolase 1, kbaZ subunit^[1], B3132^[2]^[1], YhaX^[2]^[1], KbaZ^[2]^[1], AgaZ^[2]^[1] , agaZ, ECK3120, JW3101, yhaX, b3132
Product description	G7631^[2]^[3]; Component of tagatose-1,6-bisphosphate aldolase 1^[3] Ketose bisphosphate aldolase KbaYZ subunit; required for full activity and stabilty of KbaY^[4]
EC number (for enzymes)	4.1.2.40^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of tagatose-1,6-bisphosphate aldolase 1	could be indirect
Protein	thiH		PMID:16606699	Experiment(s):EBI-1144869
Protein	phoR		PMID:16606699	Experiment(s):EBI-1144869
Protein	malZ		PMID:16606699	Experiment(s):EBI-1144869
Protein	gfcD		PMID:16606699	Experiment(s):EBI-1144869
Protein	aegA		PMID:16606699	Experiment(s):EBI-1144869
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKHLTEMVRQ HKAGKTNGIY AVCSAHPLVL EAAIRYASAN QTPLLIEATS NQVDQFGGYT GMTPADFRGF VCQLADSLNF PQDALILGGD HLGPNRWQNL PAAQAMANAD DLIKSYVAAG FKKIHLDCSM SCQDDPIPLT DDIVAERAAR LAKVAEETCL EHFGEADLEY VIGTEVPVPG GAHETLSELA VTTPDAARAT LEAHRHAFEK QGLNAIWPRI IALVVQPGVE FDHTNVIDYQ PAKASALSQM VENYETLIFE AHSTDYQTPQ SLRQLVIDHF AILKVGPALT FALREALFSL AAIEEELVPA KACSGLRQVL EDVMLDRPEY WQSHYHGDGN ARRLARGYSY SDRVRYYWPD SQIDDAFAHL VRNLADSPIP LPLISQYLPL QYVKVRSGEL QPTPRELIIN HIQDILAQYH TACEGQ
Length	426
Mol. Wt	47.192 kDa
pI	5.2 (calculated)
Extinction coefficient	47,330 - 48,205 (calc based on 17 Y, 4 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..425	PF08013 Tagatose 6 phosphate kinase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=kbaZ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131024	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947637	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010295	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P42903	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7631	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12763	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947637	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004181	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2616	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	12	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
kbaZ-agaVWA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3276916..3276956 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3132 (EcoliWiki Page)	NCBI GEO profiles for kbaZ
microarray	GenExpDB:b3132 (EcoliWiki Page)	Summary of data for kbaZ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3276212..3276638) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:H7^[7]
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Notes

Accessions Related to kbaZ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7631	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2616	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3132
EcoGene	EcoGene:EG12763	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004181	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010295	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000042214 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	AGAZ	From SHIGELLACYC
E. coli O157	AGAZ	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF08013 Tagatose 6 phosphate kinase
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

kbaZ:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to kbaZ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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