katE:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

katE

Gene Synonym(s)

ECK1730, b1732, JW1721[1], JW1721

Product Desc.

KatE[2][3];

Component of hydroperoxidase II[2]

Catalase hydroperoxidase II, heme d-containing; response to oxidative stress; chromate resistance[4]

Product Synonyms(s)

hydroperoxidase HPII(III) (catalase)[1], B1732[2][1], KatE[2][1] , ECK1730, JW1721, b1732

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): katE[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Cys438 may be blocked with a hemithioacetal group.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

katE

Mnemonic

Catalase

Synonyms

ECK1730, b1732, JW1721[1], JW1721

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

39.05 minutes 

MG1655: 1811891..1814152
<gbrowseImage> name=NC_000913:1811891..1814152 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1791132..1793393
<gbrowseImage> name=NC_012967:1791132..1793393 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1703950..1706211
<gbrowseImage> name=NC_012759:1703950..1706211 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1815581..1817842
<gbrowseImage> name=NC_007779:1815581..1817842 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1902462..1904723
<gbrowseImage> name=NC_010473:1902462..1904723 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1811891

Edman degradation

PMID:3117785


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔkatE (Keio:JW1721)

deletion

deletion

PMID:16738554

Shigen
CGSC9453[5]

katE2

CGSC:19378

ΔkatE731::kan

PMID:16738554

CGSC:101594


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1721

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCGCAACATAACGAAAAGAA

Primer 2:CCGGCAGGAATTTTGTCAATCTT

8A4

Kohara Phage

Genobase

PMID:3038334

19B6

Kohara Phage

Genobase

PMID:3038334

4F7

Kohara Phage

Genobase

PMID:3038334

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 24% [6]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[5]

est. P1 cotransduction: 47% [6]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10509

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10509

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000502

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946234

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0504

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005780

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

KatE

Synonyms

hydroperoxidase HPII(III) (catalase)[1], B1732[2][1], KatE[2][1] , ECK1730, JW1721, b1732

Product description

KatE[2][3];

Component of hydroperoxidase II[2]

Catalase hydroperoxidase II, heme d-containing; response to oxidative stress; chromate resistance[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0004096

catalase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002226

F

Seeded from EcoCyc (v14.0)

complete

GO:0004096

catalase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010582

F

Seeded from EcoCyc (v14.0)

complete

GO:0004096

catalase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011614

F

Seeded from EcoCyc (v14.0)

complete

GO:0004096

catalase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020835

F

Seeded from EcoCyc (v14.0)

complete

GO:0004096

catalase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.11.1.6

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010582

F

Seeded from EcoCyc (v14.0)

complete

GO:0004096

catalase activity

PMID:6319370

IMP: Inferred from Mutant Phenotype

F

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0006979

response to oxidative stress

PMID:6319370

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005515

protein binding

PMID:10022351

IPI: Inferred from Physical Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0020037

heme binding

PMID:7663946

IDA: Inferred from Direct Assay

F

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

PMID:7663946

IDA: Inferred from Direct Assay

F

complete

GO:0006979

response to oxidative stress

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002226

P

Seeded from EcoCyc (v14.0)

complete

GO:0042744

hydrogen peroxide catabolic process

PMID:6319370

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006979

response to oxidative stress

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010582

P

Seeded from EcoCyc (v14.0)

complete

GO:0006979

response to oxidative stress

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011614

P

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:10022351

IPI: Inferred from Physical Interaction

F

The authors refer to a paper that does not describe the method of purification of this enzyme.

Missing: with/from

GO:0006979

response to oxidative stress

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020835

P

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:3293564

IDA: Inferred from Direct Assay

C

complete

GO:0020037

heme binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010582

F

Seeded from EcoCyc (v14.0)

complete

GO:0042744

hydrogen peroxide catabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0376

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002226

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010582

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011614

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020835

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:11967071

IEP: Inferred from Expression Pattern

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of hydroperoxidase II

could be indirect

Protein

yneG

PMID:16606699

Experiment(s):EBI-1140631

Protein

lhr

PMID:16606699

Experiment(s):EBI-1140631

Protein

manC

PMID:16606699

Experiment(s):EBI-1140631

Protein

glnD

PMID:16606699

Experiment(s):EBI-1140631

Protein

groL

PMID:16606699

Experiment(s):EBI-1140631

Protein

recB

PMID:16606699

Experiment(s):EBI-1140631

Protein

ycbY

PMID:16606699

Experiment(s):EBI-1140631

Protein

fur

PMID:16606699

Experiment(s):EBI-1140631
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSQHNEKNPH QHQSPLHDSS EAKPGMDSLA PEDGSHRPAA EPTPPGAQPT APGSLKAPDT
RNEKLNSLED VRKGSENYAL TTNQGVRIAD DQNSLRAGSR GPTLLEDFIL REKITHFDHE
RIPERIVHAR GSAAHGYFQP YKSLSDITKA DFLSDPNKIT PVFVRFSTVQ GGAGSADTVR
DIRGFATKFY TEEGIFDLVG NNTPIFFIQD AHKFPDFVHA VKPEPHWAIP QGQSAHDTFW
DYVSLQPETL HNVMWAMSDR GIPRSYRTME GFGIHTFRLI NAEGKATFVR FHWKPLAGKA
SLVWDEAQKL TGRDPDFHRR ELWEAIEAGD FPEYELGFQL IPEEDEFKFD FDLLDPTKLI
PEELVPVQRV GKMVLNRNPD NFFAENEQAA FHPGHIVPGL DFTNDPLLQG RLFSYTDTQI
SRLGGPNFHE IPINRPTCPY HNFQRDGMHR MGIDTNPANY EPNSINDNWP RETPPGPKRG
GFESYQERVE GNKVRERSPS FGEYYSHPRL FWLSQTPFEQ RHIVDGFSFE LSKVVRPYIR
ERVVDQLAHI DLTLAQAVAK NLGIELTDDQ LNITPPPDVN GLKKDPSLSL YAIPDGDVKG
RVVAILLNDE VRSADLLAIL KALKAKGVHA KLLYSRMGEV TADDGTVLPI AATFAGAPSL
TVDAVIVPCG NIADIADNGD ANYYLMEAYK HLKPIALAGD ARKFKATIKI ADQGEEGIVE
ADSADGSFMD ELLTLMAAHR VWSRIPKIDK IPA
Length

753

Mol. Wt

84.164 kDa

pI

5.8 (calculated)

Extinction coefficient

77,810 - 78,060 (calc based on 19 Y, 9 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

81..469

PF00199 Catalase

PMID:19920124

Domain

495..563

PF06628 Catalase-related immune-responsive

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=katE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176140

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946234

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005780

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P21179

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10509

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10509

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946234

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000502

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0504

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

149.12+/-0.75

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.6485+/-0.14301

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.157231314

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

26

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1967

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

15a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

katE

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1811871..1811911 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1732 (EcoliWiki Page)

NCBI GEO profiles for katE

microarray

GenExpDB:b1732 (EcoliWiki Page)

Summary of data for katE from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1811631..1811942) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ13; Well:E4[7]

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Notes

Accessions Related to katE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10509

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0504

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1732

EcoGene

EcoGene:EG10509

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000502

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005780

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

KATE

From SHIGELLACYC

E. coli O157

KATE

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00199 Catalase

Superfamily (EcoliWiki Page)

SUPERFAMILY:52317

Panther (EcoliWiki Page)

PTHR11465:SF0

Superfamily (EcoliWiki Page)

SUPERFAMILY:56634

Pfam (EcoliWiki Page)

PF06628 Catalase-related immune-responsive

EcoCyc

EcoCyc:EG10509

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10509

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000502

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0504

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005780

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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