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Standard Name	intB
Gene Synonym(s)	ECK4264, b4271, JW4227, int(P4), yjgC^[1], int, yjgC
Product Desc.	KpLE2 phage-like element; predicted integrase^[2]^[3]
Product Synonyms(s)	KpLE2 phage-like element; predicted integrase^[1], B4271^[2]^[1], YjgC^[2]^[1], IntB^[2]^[1] , ECK4264, int, JW4227, yjgC, b4271
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): intB^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	intB
Mnemonic
Synonyms	ECK4264, b4271, JW4227, int(P4), yjgC^[1], int, yjgC
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	96.88 minutes, 96.88 minutes	MG1655: 4494773..4495963 <gbrowseImage> name=NC_000913:4494773..4495963 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4479978..4481168 <gbrowseImage> name=NC_012967:4479978..4481168 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 4501430..4502620 <gbrowseImage> name=NC_007779:4501430..4502620 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4596581..4597771 <gbrowseImage> name=NC_010473:4596581..4597771 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔintB (Keio:JW4227)	deletion	deletion	PMID:16738554	Shigen CGSC11040^[4]
ΔintB-741::kan			PMID:16738554	CGSC:101572
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4227	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCATCTGCTTGTCCATCCAAA Primer 2:CCtTGTTTTAATGGATTATTAAT
E4D8	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[4]	est. P1 cotransduction: 10% ^[5] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[4]	est. P1 cotransduction: % ^[5] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12364	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12364	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002232	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948782	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2267	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013992	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	IntB
Synonyms	KpLE2 phage-like element; predicted integrase^[1], B4271^[2]^[1], YjgC^[2]^[1], IntB^[2]^[1] , ECK4264, int, JW4227, yjgC, b4271
Product description	KpLE2 phage-like element; predicted integrase^[2]^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011010	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0229	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1147790
Protein	groL	PMID:16606699	Experiment(s):EBI-1147790
Protein	napA	PMID:16606699	Experiment(s):EBI-1147790
Protein	fumC	PMID:16606699	Experiment(s):EBI-1147790
Protein	rplB	PMID:16606699	Experiment(s):EBI-1147790
Protein	clpB	PMID:16606699	Experiment(s):EBI-1147790
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MHLLVHPNGS KYWRLQYRYE GKQKMLALGV YPEITLADAR VRRDEARKLL ANGVDPGDKK KNDKVEQSKA RTFKEVAIEW HGTNKKWSED HAHRVLKSLE DNLFAALGER NIAELKTRDL LAPIKAVEMS GRLEVAARLQ QRTTAIMRYA VQSGLIDYNP AQEMAGAVAS CNRQHRPALE LKRIPELLTK IDSYTGRPLT RWAIELTLLI FIRSSELRFA RWSEIDFEAS IWTIPPEREP IPGVKHSHRG SKMRTTHLVP LSTQALAILK QIKQFYGAHD LIFIGDHDSH KPMSENTVNS ALRVMGYDTK VEVCGHGFRT MACSSLVESG LWSRDAVERQ MSHMARNSVR AAYIHKAEHL EERRLMLQWW ADFLDVNRER FISPFEYAKI NNPLKQ
Length	396
Mol. Wt	45.583 kDa
pI	9.9 (calculated)
Extinction coefficient	65,890 - 66,265 (calc based on 11 Y, 9 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	177..360	PF00589 Phage integrase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=intB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
ASAP	ASAP:ABE-0013992	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948782	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39347	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12364	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12364	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948782	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002232	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2267	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.41E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
intB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4494753..4494793 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4271 (EcoliWiki Page)	NCBI GEO profiles for intB
microarray	GenExpDB:b4271 (EcoliWiki Page)	Summary of data for intB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4493966..4494355) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:F11^[6]
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Notes

Accessions Related to intB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12364	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2267	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4271
EcoGene	EcoGene:EG12364	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002232	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013992	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001970 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S4839	From SHIGELLACYC
E. coli O157	Z4313	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00589 Phage integrase family
EcoCyc	EcoCyc:EG12364	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12364	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002232	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2267	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013992	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

intB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to intB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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