insO-2:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insO-2

Mnemonic
Synonyms

insO, ECK0259, b0257, JW0247, ECK4274, b4285, JW4245, insO-1[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.84 minutes 

MG1655: 271054..271479
<gbrowseImage> name=NC_000913:271054..271479 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 271054..271479
<gbrowseImage> name=NC_007779:271054..271479 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

97.14 minutes 

MG1655: 4506981..4507577
<gbrowseImage> name=NC_000913:4506981..4507577 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4513638..4514234
<gbrowseImage> name=NC_007779:4513638..4514234 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4608789..4609385
<gbrowseImage> name=NC_010473:4608789..4609385 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

b0257::Tn5KAN-I-SceI (FB20103)

Insertion at nt 362 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20103

contains pKD46

ΔinsO774::kan

PMID:16738554[5]

CGSC:101568


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[6]

Status:

Primer 1:

Primer 2:

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[7]

est. P1 cotransduction: 4% [8]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[7]

est. P1 cotransduction: % [8]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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