insH-11:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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<protect> See Help:Quickview for help with entering information in the Quickview table.

Standard Name

insH-11

Gene Synonym(s)

insH, ECK0261, b0259, JW0250, trs5, yi52_1, trs5-1, b0552, JW0540, yi52_2, trs5-2, b0656, JW0652, yi52_3, trs5-3, b1331, JW1324, yi52_4, trs5-4, b1370, JW1363, yi52_5, trs5-5, b1994, JW1972, yi52_6, trs5-6, b2030, JW2014, yi52_7, trs5-7, b2192, JW2179, yi52_8, trs5-8, b2982, JW2949, yi52_9, trs5-9, b3218, JW3185, trs5-10, yi52_, b3505, JW3472, trs5-11, unique to W3110, JW5951, JW5936, JW5935, JW5928, JW5914, JW5895, JW1882, insH-1, insH-2, insH-3, insH-4, insH-5, insH-6, insH-7, insH-8, insH-9, insH-10, yi52_10, yi52_11[1][2][3]

Product Desc.

IS5 transposase and trans-activator[3][2]

Product Synonyms(s)

B1370[3], InsH-5[3], Trs5-5[3], Yi52_5[3]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): insH-1[3]

Regulation/Activity
Quick Links

PortEco search(insH-11)    DNA display    Protein display    Pubmed(insH-11)    Textpresso(insH-11)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insH-11

Mnemonic
Synonyms

insH, ECK0261, b0259, JW0250, trs5, yi52_1, trs5-1, b0552, JW0540, yi52_2, trs5-2, b0656, JW0652, yi52_3, trs5-3, b1331, JW1324, yi52_4, trs5-4, b1370, JW1363, yi52_5, trs5-5, b1994, JW1972, yi52_6, trs5-6, b2030, JW2014, yi52_7, trs5-7, b2192, JW2179, yi52_8, trs5-8, b2982, JW2949, yi52_9, trs5-9, b3218, JW3185, trs5-10, yi52_, b3505, JW3472, trs5-11, unique to W3110, JW5951, JW5936, JW5935, JW5928, JW5914, JW5895, JW1882, insH-1, insH-2, insH-3, insH-4, insH-5, insH-6, insH-7, insH-8, insH-9, insH-10, yi52_10, yi52_11[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.89 minutes 

MG1655: 274341..273325
<gbrowseImage> name=NC_000913:273325..274341 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1284949..1285965
<gbrowseImage> name=NC_012759:1284949..1285965 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1288499..1289526
<gbrowseImage> name=NC_012759:1288499..1289526 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1317829..1318809
<gbrowseImage> name=NC_012759:1317829..1318809 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1956812..1957828
<gbrowseImage> name=NC_012759:1956812..1957828 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1992402..1993418
<gbrowseImage> name=NC_012759:1992402..1993418 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2172892..2173908
<gbrowseImage> name=NC_012759:2172892..2173908 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 273324..274340
<gbrowseImage> name=NC_012759:273324..274340 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3015348..3016364
<gbrowseImage> name=NC_012759:3015348..3016364 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3250872..3251888
<gbrowseImage> name=NC_012759:3250872..3251888 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3538699..3539715
<gbrowseImage> name=NC_012759:3538699..3539715 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 476720..477736
<gbrowseImage> name=NC_012759:476720..477736 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 589980..590996
<gbrowseImage> name=NC_012759:589980..590996 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 274341..273325
<gbrowseImage> name=NC_007779:273325..274341 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

12.37 minutes 

MG1655: 574976..573960
<gbrowseImage> name=NC_000913:573960..574976 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 574976..573960
<gbrowseImage> name=NC_007779:573960..574976 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

14.81 minutes 

MG1655: 688236..687220
<gbrowseImage> name=NC_000913:687220..688236 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 689435..688419
<gbrowseImage> name=NC_007779:688419..689435 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

30.05 minutes 

MG1655: 1394100..1395116
<gbrowseImage> name=NC_000913:1394100..1395116 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1397790..1398806
<gbrowseImage> name=NC_007779:1397790..1398806 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

30.73 minutes 

MG1655: 1426750..1425770
<gbrowseImage> name=NC_000913:1425770..1426750 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1430440..1429460
<gbrowseImage> name=NC_007779:1429460..1430440 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

44.49 minutes 

MG1655: 2065345..2064329
<gbrowseImage> name=NC_000913:2064329..2065345 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2069458..2068442
<gbrowseImage> name=NC_007779:2068442..2069458 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

45.26 minutes 

MG1655: 2100935..2099919
<gbrowseImage> name=NC_000913:2099919..2100935 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2105048..2104032
<gbrowseImage> name=NC_007779:2104032..2105048 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

49.29 minutes 

MG1655: 2288103..2287087
<gbrowseImage> name=NC_000913:2287087..2288103 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2293415..2292399
<gbrowseImage> name=NC_007779:2292399..2293415 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

67.42 minutes 

MG1655: 3128200..3129216
<gbrowseImage> name=NC_000913:3128200..3129216 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3128834..3129850
<gbrowseImage> name=NC_007779:3128834..3129850 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

72.5 minutes 

MG1655: 3364740..3363724
<gbrowseImage> name=NC_000913:3363724..3364740 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3366573..3365557
<gbrowseImage> name=NC_007779:3365557..3366573 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

78.67 minutes 

MG1655: 3651221..3650205
<gbrowseImage> name=NC_000913:3650205..3651221 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3987217..3988233
<gbrowseImage> name=NC_007779:3987217..3988233 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4310431..4311411
<gbrowseImage> name=NC_007779:4310431..4311411 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3750659..3751639
<gbrowseImage> name=NC_007779:3750659..3751639 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3746979..3747959
<gbrowseImage> name=NC_007779:3746979..3747959 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3261202..3262182
<gbrowseImage> name=NC_007779:3261202..3262182 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2177051..2178031
<gbrowseImage> name=NC_007779:2177051..2178031 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 654281..655261
<gbrowseImage> name=NC_007779:654281..655261 source=W3110 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1981371..1980355
<gbrowseImage> name=NC_007779:1980355..1981371 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3748966..3747950
<gbrowseImage> name=NC_010473:3747950..3748966 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments
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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

5A5

Kohara Phage

Genobase

PMID:3038334

3C7

Kohara Phage

Genobase

PMID:3038334

23E10

Kohara Phage

Genobase

PMID:3038334

1G6

Kohara Phage

Genobase

PMID:3038334

3A2

Kohara Phage

Genobase

PMID:3038334

16A8

Kohara Phage

Genobase

PMID:3038334

6B4

Kohara Phage

Genobase

PMID:3038334

3G3

Kohara Phage

Genobase

PMID:3038334

2C3

Kohara Phage

Genobase

PMID:3038334

4E7

Kohara Phage

Genobase

PMID:3038334

7F11

Kohara Phage

Genobase

PMID:3038334

18A9

Kohara Phage

Genobase

PMID:3038334

9B2

Kohara Phage

Genobase

PMID:3038334

3B12

Kohara Phage

Genobase

PMID:3038334

21H10

Kohara Phage

Genobase

PMID:3038334

1G1

Kohara Phage

Genobase

PMID:3038334

10H4

Kohara Phage

Genobase

PMID:3038334

8F4

Kohara Phage

Genobase

PMID:3038334

20F6

Kohara Phage

Genobase

PMID:3038334

19D1

Kohara Phage

Genobase

PMID:3038334

1G7

Kohara Phage

Genobase

PMID:3038334

12F3

Kohara Phage

Genobase

PMID:3038334

2F9

Kohara Phage

Genobase

PMID:3038334

2B7

Kohara Phage

Genobase

PMID:3038334

8F8

Kohara Phage

Genobase

PMID:3038334

8D6

Kohara Phage

Genobase

PMID:3038334

7C8

Kohara Phage

Genobase

PMID:3038334

2A2

Kohara Phage

Genobase

PMID:3038334

2A2

Kohara Phage

Genobase

PMID:3038334

3F11

Kohara Phage

Genobase

PMID:3038334

3F11

Kohara Phage

Genobase

PMID:3038334

4D9

Kohara Phage

Genobase

PMID:3038334

12E4

Kohara Phage

Genobase

PMID:3038334

12A6

Kohara Phage

Genobase

PMID:3038334

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: 5% [5]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: % [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:G6134

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG40008

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944941

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6308

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946163

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6360

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944917

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6667

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945896

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6693

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945944

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7074

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946507

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7092

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946577

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7150

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949121

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7548

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947516

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7672

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947743

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7769

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948015

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

InsH-4

Synonyms

B1370[3], InsH-5[3], Trs5-5[3], Yi52_5[3] , ECK0261, b0259, b0552, b0656, b1331, b1370, b1994, b2030, b2192, b2982, b3218, b3505

Product description

IS5 transposase and trans-activator[3][2]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology

IS5 transposase and trans-activator

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
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Interactions

IS5 transposase and trans-activator

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSHQLTFADS EFSTKRRQTR KEIFLSRMEQ ILPWQNMTAV IEPFYPKAGN GRRPYPLETM
LRIHCMQHWY NLSDGAMEDA LYEIASMRLF ARLSLDSALP DRTTIMNFRH LLEQHQLARQ
LFKTINRWLA EAGVMMTQGT LVDATIIEAP SSTKNKEQQR DPEMHQTKKG NQWHFGMKAH
IGVDAKSGLT HSLVTTAANE HDLNQLGNLL HGEEQFVSAD AGYQGAPQRE ELAEVDVDWL
IAERPGKVKT LKQNPRKNKT AINIEYMKAS IRARVEHPFR IIKRQFGFVK ARYKGLLKND
NQLAMLFTLA NLFRVDQMIR QWERSQ
Length

326

Mol. Wt

37.777 kDa

pI

9.9 (calculated)

Extinction coefficient

43,430 - 43,555 (calc based on 7 Y, 6 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

136..313

PF01609 Transposase DDE domain

PMID:19920124

Domain

51..126

PF05598 Transposase domain (DUF772)

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=insH-11 taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128244

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944941

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16128535

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946163

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16128639

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944917

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16129292

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945896

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16129331

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945944

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16129935

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946507

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16129971

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946577

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16130129

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949121

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16130882

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947516

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16131108

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947743

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:16131377

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948015

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0000888

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6134

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG40008

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944941

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0001886

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6308

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946163

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0002246

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6360

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944917

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0004467

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6667

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945896

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0004586

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6693

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945944

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0006619

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7074

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946507

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0006742

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7092

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946577

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0007252

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7150

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949121

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0009783

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7548

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947516

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0010559

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7672

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947743

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0011446

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7769

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948015

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

21

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

202

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

insH-1

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:274321..274361 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3505 (EcoliWiki Page)

NCBI GEO profiles for insH-11

microarray

GenExpDB:b3505 (EcoliWiki Page)

Summary of data for insH-11 from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (274256..688569) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ14; Well:G3[6]

GFP Fusion

Intergenic region (274256..274573) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ17; Well:D8[6]

GFP Fusion

Intergenic region (1393853..1394168) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ05; Well:B11[6]

GFP Fusion

Intergenic region (274256..2288567) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ04; Well:B4[6]

GFP Fusion

Intergenic region (274256..3364578) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ18; Well:A2[6]

GFP Fusion

Intergenic region (274256..3651631) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ19; Well:B8[6]

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Notes

Accessions Related to insH-11 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7769

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3505

EchoBASE (EcoliWiki Page)

EchoBASE:EB4730


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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments
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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01609 Transposase DDE domain

Pfam (EcoliWiki Page)

PF05598 Transposase domain (DUF772)


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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