insE-5:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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<protect> See Help:Quickview for help with entering information in the Quickview table.

Standard Name

insE-5

Gene Synonym(s)

insE, ECK0298, b0298, JW5036, insE1, b0373, JW5050, insE2, b0540, JW5074, insE3, b1027, JW5144, insE4, b2088, JW5341, insE5, insE-1, insE-2, insE-3, insE-4[1][2][3]

Product Desc.

IS3 element protein InsE[3][2]

Product Synonyms(s)

IS3 element protein[1], DLP12 prophage; IS3 element protein InsE[1], IS3 element protein InsE[1], B0373[3][1], B0298[3][1], B2088[3][1], B0540[3][1], B1027[3][1], InsE-1[3][1], InsE-2[3][1], InsE-3[3][1], InsE-4[3][1], InsE-5[3][1]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): eaeH[3], insEF-2[3], insEF-3[3], insEF-4[3], insEF-5[3]

Regulation/Activity
Quick Links

PortEco search(insE-5)    DNA display    Protein display    Pubmed(insE-5)    Textpresso(insE-5)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insE-5

Mnemonic
Synonyms

insE, ECK0298, b0298, JW5036, insE1, b0373, JW5050, insE2, b0540, JW5074, insE3, b1027, JW5144, insE4, b2088, JW5341, insE5, insE-1, insE-2, insE-3, insE-4[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

6.78 minutes 

MG1655: 314506..314814
<gbrowseImage> name=NC_000913:314506..314814 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2882765..2883073
<gbrowseImage> name=NC_012967:2882765..2883073 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1615527..1615835
<gbrowseImage> name=NC_012967:1615527..1615835 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1607973..1608281
<gbrowseImage> name=NC_012967:1607973..1608281 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1442692..1443000
<gbrowseImage> name=NC_012967:1442692..1443000 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1111423..1111731
<gbrowseImage> name=NC_012967:1111423..1111731 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2060734..2061042
<gbrowseImage> name=NC_012759:2060734..2061042 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 294585..294893
<gbrowseImage> name=NC_012759:294585..294893 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 468816..469124
<gbrowseImage> name=NC_012759:468816..469124 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 998105..998413
<gbrowseImage> name=NC_012759:998105..998413 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 314506..314814
<gbrowseImage> name=NC_007779:314506..314814 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

8.45 minutes 

MG1655: 392134..391826
<gbrowseImage> name=NC_000913:391826..392134 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 392134..391826
<gbrowseImage> name=NC_007779:391826..392134 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

12.2 minutes 

MG1655: 566056..566364
<gbrowseImage> name=NC_000913:566056..566364 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 566056..566364
<gbrowseImage> name=NC_007779:566056..566364 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

23.59 minutes 

MG1655: 1094669..1094361
<gbrowseImage> name=NC_000913:1094361..1094669 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1095868..1095560
<gbrowseImage> name=NC_007779:1095560..1095868 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

46.73 minutes 

MG1655: 2168251..2168559
<gbrowseImage> name=NC_000913:2168251..2168559 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2172364..2172672
<gbrowseImage> name=NC_007779:2172364..2172672 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2259259..2259567
<gbrowseImage> name=NC_010473:2259259..2259567 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

3E3

Kohara Phage

Genobase

PMID:3038334

9G4

Kohara Phage

Genobase

PMID:3038334

6E2

Kohara Phage

Genobase

PMID:3038334

1A10

Kohara Phage

Genobase

PMID:3038334

2C4

Kohara Phage

Genobase

PMID:3038334

1E12

Kohara Phage

Genobase

PMID:3038334

9G7

Kohara Phage

Genobase

PMID:3038334

1H4

Kohara Phage

Genobase

PMID:3038334

10H4

Kohara Phage

Genobase

PMID:3038334

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[4]

est. P1 cotransduction: % [5]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[4]

est. P1 cotransduction: 77% [5]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:G6168

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG40005

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944952

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6224

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946900

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6296

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945759

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6534

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945584

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7125

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946616

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

InsE

Synonyms

IS3 element protein[1], DLP12 prophage; IS3 element protein InsE[1], IS3 element protein InsE[1], B0373[3][1], B0298[3][1], B2088[3][1], B0540[3][1], B1027[3][1], InsE-1[3][1], InsE-2[3][1], InsE-3[3][1], InsE-4[3][1], InsE-5[3][1] , ECK0298, b0298, b0373, b0540, b1027, b2088

Product description

IS3 element protein InsE[3][2]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

 
Length
Mol. Wt
pI
Extinction coefficient



Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=insE-5 taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:226524700

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944952

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:226524701

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946900

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:226524703

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945759

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:226524706

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945584

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GI:226524731

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946616

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0001028

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6168

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG40005

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944952

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0001279

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6224

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946900

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0001855

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6296

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945759

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0003477

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G6534

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945584

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0006917

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

ECOCYC:G7125

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946616

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

6a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

76a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

eaeH

insEF-2

insEF-3

insEF-4

insEF-5

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:314486..314526 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2088 (EcoliWiki Page)

NCBI GEO profiles for insE-5

microarray

GenExpDB:b2088 (EcoliWiki Page)

Summary of data for insE-5 from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (314369..314545) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ11; Well:F7[6]

GFP Fusion

Intergenic region (392043..392289) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ13; Well:G12[6]

GFP Fusion

Intergenic region (565498..314545) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ14; Well:A5[6]

GFP Fusion

Intergenic region (392043..1094746) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ16; Well:C5[6]

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Notes

Accessions Related to insE-5 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7125

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2088


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

S1094

From SHIGELLACYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 3.28 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. 6.0 6.1 6.2 6.3 Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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