infB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

infB

Mnemonic

Initiation factor

Synonyms

ECK3157, b3168, JW3137, ssyG[1], gicD, ssyG

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

71.37 minutes 

MG1655: 3314036..3311364
<gbrowseImage> name=NC_000913:3311364..3314036 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3251248..3248576
<gbrowseImage> name=NC_012967:3248576..3251248 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3198512..3201184
<gbrowseImage> name=NC_012759:3198512..3201184 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3315869..3313197
<gbrowseImage> name=NC_007779:3313197..3315869 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3411781..3409109
<gbrowseImage> name=NC_010473:3409109..3411781 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3311364

Edman degradation

PMID:3894004[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

infBG684A

G684A

(in strain: ECOAU9306)

Strain variation; seeded from UniProt:P0A705

infBD409E

D409E

(in strain: IQ489)

Strain variation; seeded from UniProt:P0A705

infBG423GG

G423GG

(in strain: IQ490)

Strain variation; seeded from UniProt:P0A705

infBH432Q

H432Q

(in strain: ECOAU9326)

Strain variation; seeded from UniProt:P0A705

infBQ490G

Q490G

(in strain: ECOAU9302, ECOAU9306, ECOAU9307 and ECOAU9309)

Strain variation; seeded from UniProt:P0A705

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3137

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCACAGATGTAACGATTAAAAC

Primer 2:CCAGCAATGGTACGTTGGATCTC

E4G11S

Kohara Phage

Genobase

PMID:3038334[4]

18H7

Kohara Phage

Genobase

PMID:3038334[4]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[5]

est. P1 cotransduction: % [6]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[5]

est. P1 cotransduction: 48% [6]
Synonyms:zgj-203::Tn10, zha-203::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10505

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10505

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000498

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947684

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0500

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010410

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Plumbridge, JA et al. (1985) Two translational initiation sites in the infB gene are used to express initiation factor IF2 alpha and IF2 beta in Escherichia coli. EMBO J. 4 223-9 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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