infB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

InfB

Synonyms

fused protein chain initiation factor 2, IF2: membrane protein[1], conserved protein[1], B3168[2][1], SsyG[2][1], InfB[2][1], IF2[2][1] , ECK3157, gicD, JW3137, ssyG, b3168

Product description

protein chain initiation factor IF-2[2][3]

Protein chain initiation factor IF2[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003743

translation initiation factor activity

PMID:3894004[5]

IDA: Inferred from Direct Assay

F

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009061

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003743

translation initiation factor activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00100

F

Seeded from EcoCyc (v14.0)

complete

GO:0003743

translation initiation factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000178

F

Seeded from EcoCyc (v14.0)

complete

GO:0003743

translation initiation factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006847

F

Seeded from EcoCyc (v14.0)

complete

GO:0003743

translation initiation factor activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0396

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00100

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000178

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000795

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004161

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005225

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000178

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006413

translational initiation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00100

P

Seeded from EcoCyc (v14.0)

complete

GO:0006413

translational initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000178

P

Seeded from EcoCyc (v14.0)

complete

GO:0006413

translational initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006847

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726[6]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

ribD

PMID:15690043[7]

Experiment(s):EBI-889882

Protein

rplC

PMID:15690043[7]

Experiment(s):EBI-889882, EBI-894820

Protein

rplM

PMID:15690043[7]

Experiment(s):EBI-889882

Protein

metN

PMID:15690043[7]

Experiment(s):EBI-894820

Protein

dnaK

PMID:15690043[7]

Experiment(s):EBI-894820

Protein

grpE

PMID:15690043[7]

Experiment(s):EBI-894820

Protein

phoA

PMID:15690043[7]

Experiment(s):EBI-894820

Protein

rpmG

PMID:15690043[7]

Experiment(s):EBI-894820

Protein

adhP

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

phoA

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

ygfZ

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

rpsE

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

rhlE

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

rplJ

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

arpA

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

uup

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

greA

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

yedJ

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

ybiB

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

mhpR

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

artM

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

rplF

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

ebgC

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

fliJ

PMID:16606699[8]

Experiment(s):EBI-1144996

Protein

tig

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

grpE

PMID:19402753[9]

LCMS(ID Probability):99.0

Protein

aspS

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

pykA

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

phoA

PMID:19402753[9]

LCMS(ID Probability):99.0

Protein

zwf

PMID:19402753[9]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTDVTIKTLA AERQTSVERL VQQFADAGIR KSADDSVSAQ EKQTLIDHLN QKNSGPDKLT
LQRKTRSTLN IPGTGGKSKS VQIEVRKKRT FVKRDPQEAE RLAAEEQAQR EAEEQARREA
EESAKREAQQ KAEREAAEQA KREAAEQAKR EAAEKDKVSN QQDDMTKNAQ AEKARREQEA
AELKRKAEEE ARRKLEEEAR RVAEEARRMA EENKWTDNAE PTEDSSDYHV TTSQHARQAE
DESDREVEGG RGRGRNAKAA RPKKGNKHAE SKADREEARA AVRGGKGGKR KGSSLQQGFQ
KPAQAVNRDV VIGETITVGE LANKMAVKGS QVIKAMMKLG AMATINQVID QETAQLVAEE
MGHKVILRRE NELEEAVMSD RDTGAAAEPR APVVTIMGHV DHGKTSLLDY IRSTKVASGE
AGGITQHIGA YHVETENGMI TFLDTPGHAA FTSMRARGAQ ATDIVVLVVA ADDGVMPQTI
EAIQHAKAAQ VPVVVAVNKI DKPEADPDRV KNELSQYGIL PEEWGGESQF VHVSAKAGTG
IDELLDAILL QAEVLELKAV RKGMASGAVI ESFLDKGRGP VATVLVREGT LHKGDIVLCG
FEYGRVRAMR NELGQEVLEA GPSIPVEILG LSGVPAAGDE VTVVRDEKKA REVALYRQGK
FREVKLARQQ KSKLENMFAN MTEGEVHEVN IVLKADVQGS VEAISDSLLK LSTDEVKVKI
IGSGVGGITE TDATLAAASN AILVGFNVRA DASARKVIEA ESLDLRYYSV IYNLIDEVKA
AMSGMLSPEL KQQIIGLAEV RDVFKSPKFG AIAGCMVTEG VVKRHNPIRV LRDNVVIYEG
ELESLRRFKD DVNEVRNGME CGIGVKNYND VRTGDVIEVF EIIEIQRTIA
Length

890

Mol. Wt

97.348 kDa

pI

6.0 (calculated)

Extinction coefficient

27,390 - 27,765 (calc based on 11 Y, 2 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

389..554

PF00009 Elongation factor Tu GTP binding domain

PMID:19920124[10]

Domain

579..642

PF03144 Elongation factor Tu domain 2

PMID:19920124[10]

Domain

810..878

PF03144 Elongation factor Tu domain 2

PMID:19920124[10]

Domain

1..52

PF04760 Translation initiation factor IF-2, N-terminal region

PMID:19920124[10]

Domain

313..364

PF04760 Translation initiation factor IF-2, N-terminal region

PMID:19920124[10]

Domain

55..95

PF08364 Bacterial translation initiation factor IF-2 associated region

PMID:19920124[10]

Domain

672..780

PF11987 Translation-initiation factor 2

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=infB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131060

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947684

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010410

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A705

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10505

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10505

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947684

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000498

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0500

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Plumbridge, JA et al. (1985) Two translational initiation sites in the infB gene are used to express initiation factor IF2 alpha and IF2 beta in Escherichia coli. EMBO J. 4 223-9 PubMed
  6. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 10.5 10.6 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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