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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	ilvA
Gene Synonym(s)	ECK3764, b3772, JW3745, ile^[1]
Product Desc.	IlvA^[2]^[3]; Component of threonine dehydratase (biosynthetic)^[2]^[3] Threonine deaminase; also known as threonine dehydratase, biosynthetic^[4]
Product Synonyms(s)	threonine deaminase^[1], B3772^[2]^[1], Ile^[2]^[1], IlvA^[2]^[1] , ECK3764, JW3745, b3772
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ilvA^[2], ilvEDA^[2], ilvLG_1G_2MEDA^[2], ilvLGGMEDA, ilvLGMEDA, ilvGMEDA
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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N-terminal sequence of cloned IlvA shows fMet is clipped, but natural RBS was not used (Eisenstein, 1991) so it is not Verified, but the ATG start is reliable based on comparisons to Salmonella. ACT-like domain (Grant, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ilvA
Mnemonic	Isoleucine-valine (requirement)
Synonyms	ECK3764, b3772, JW3745, ile^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	85.21 minutes	MG1655: 3953354..3954898 <gbrowseImage> name=NC_000913:3953354..3954898 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3917133..3918677 <gbrowseImage> name=NC_012967:3917133..3918677 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3843023..3844567 <gbrowseImage> name=NC_012759:3843023..3844567 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3681350..3679806 <gbrowseImage> name=NC_007779:3679806..3681350 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4052274..4053818 <gbrowseImage> name=NC_010473:4052274..4053818 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability
ΔilvA (Keio:JW3745)	deletion	deletion		PMID:16738554	Shigen CGSC10733^[5]
ilvA296					CGSC:5345
ilvA634					CGSC:5682
ilvA215					CGSC:8360
ilvA210(ts)			temperature sensitive		CGSC:9349
ilvA201					CGSC:9358
ilvA681					CGSC:10343
ilvA0					CGSC:10797
ilvA194			? Also affects ilvY?		CGSC:13261
ilvA24				PMID:4550818	CGSC:13325
ilvA202					CGSC:13344
ilvA206				PMID:4570785	CGSC:13350
ilvA1000					CGSC:73759
ilvA654					CGSC:74043
ΔilvA723::kan				PMID:16738554	CGSC:101542
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3745	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCTGACTCGCAACCCCTGTC Primer 2:CCACCCGCCAAAAAGAACCTGAA
2A8	Kohara Phage	Genobase	PMID:3038334
2E6	Kohara Phage	Genobase	PMID:3038334
ilvD500::Tn10	Linked marker	CAG18431 = CGSC7462^[5]	est. P1 cotransduction: 99% ^[6] Synonyms:ilv500::Tn10
metEo-3079::Tn10	Linked marker	CAG18491 = CGSC7464^[5]	est. P1 cotransduction: 7% ^[6] Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10493	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10493	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000486	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948287	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0488	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012321	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	IlvA
Synonyms	threonine deaminase^[1], B3772^[2]^[1], Ile^[2]^[1], IlvA^[2]^[1] , ECK3764, JW3745, b3772
Product description	IlvA^[2]^[3]; Component of threonine dehydratase (biosynthetic)^[2]^[3] Threonine deaminase; also known as threonine dehydratase, biosynthetic^[4]
EC number (for enzymes)	4.3.1.19^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004794	L-threonine ammonia-lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001721	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004794	L-threonine ammonia-lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005787	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004794	L-threonine ammonia-lyase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.3.1.19	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009097	isoleucine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001721	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009097	isoleucine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005787	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009097	isoleucine biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0412	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016597	amino acid binding	PMID:2005118	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0030170	pyridoxal phosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000634	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030170	pyridoxal phosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001926	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009097	isoleucine biosynthetic process	PMID:4604254	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0004794	L-threonine ammonia-lyase activity	PMID:4604254	IDA: Inferred from Direct Assay		F		complete
	GO:0009082	branched chain family amino acid biosynthetic process	PMID:348689	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0009097	isoleucine biosynthetic process	PMID:8407838	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0004794	L-threonine ammonia-lyase activity	PMID:8407838	IDA: Inferred from Direct Assay		F		complete
	GO:0005737	cytoplasm	PMID:2005118	IC: Inferred by Curator		C		Missing: with/from
	GO:0004794	L-threonine ammonia-lyase activity	PMID:2005118	IDA: Inferred from Direct Assay		F		complete
	GO:0016597	amino acid binding	PMID:2005118	IDA: Inferred from Direct Assay		F	L-Valine activates IlvA activity. CHEBI:16414	complete
	GO:0016597	amino acid binding	PMID:2005118	IDA: Inferred from Direct Assay		F	L-isoleucine binding negatively regulates IlvA activity. CHEBI:17191	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of threonine dehydratase (biosynthetic)	could be indirect
Protein	ppc		PMID:16606699	Experiment(s):EBI-1146658
Protein	rpoC		PMID:16606699	Experiment(s):EBI-1146658
Protein	rpsC		PMID:16606699	Experiment(s):EBI-1146658
Protein	rne		PMID:16606699	Experiment(s):EBI-1146658
Protein	yfaS		PMID:16606699	Experiment(s):EBI-1146658
Protein	katG		PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):27.215567
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MADSQPLSGA PEGAEYLRAV LRAPVYEAAQ VTPLQKMEKL SSRLDNVILV KREDRQPVHS FKLRGAYAMM AGLTEEQKAH GVITASAGNH AQGVAFSSAR LGVKALIVMP TATADIKVDA VRGFGGEVLL HGANFDEAKA KAIELSQQQG FTWVPPFDHP MVIAGQGTLA LELLQQDAHL DRVFVPVGGG GLAAGVAVLI KQLMPQIKVI AVEAEDSACL KAALDAGHPV DLPRVGLFAE GVAVKRIGDE TFRLCQEYLD DIITVDSDAI CAAMKDLFED VRAVAEPSGA LALAGMKKYI ALHNIRGERL AHILSGANVN FHGLRYVSER CELGEQREAL LAVTIPEEKG SFLKFCQLLG GRSVTEFNYR FADAKNACIF VGVRLSRGLE ERKEILQMLN DGGYSVVDLS DDEMAKLHVR YMVGGRPSHP LQERLYSFEF PESPGALLRF LNTLGTYWNI SLFHYRSHGT DYGRVLAAFE LGDHEPDFET RLNELGYDCH DETNNPAFRF FLAG
Length	514
Mol. Wt	56.195 kDa
pI	5.8 (calculated)
Extinction coefficient	31,860 - 32,735 (calc based on 14 Y, 2 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	25..316	PF00291 Pyridoxal-phosphate dependent enzyme	PMID:19920124
Domain	329..419	PF00585 C-terminal regulatory domain of Threonine dehydratase	PMID:19920124
Domain	424..512	PF00585 C-terminal regulatory domain of Threonine dehydratase	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ilvA taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131630	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948287	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012321	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P04968	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10493	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10493	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948287	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000486	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0488	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	10.817+/-0.145	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.030764249	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	346	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	400	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	420	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ilvA
ilvEDA
ilvLG_1G_2MEDA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3953334..3953374 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3772 (EcoliWiki Page)	NCBI GEO profiles for ilvA
microarray	GenExpDB:b3772 (EcoliWiki Page)	Summary of data for ilvA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to ilvA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10493	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0488	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3772
EcoGene	EcoGene:EG10493	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000486	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012321	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT3G10050 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000005532 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000028425 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Homo sapiens	ENSP00000339435 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000027194 (score: 1.000; bootstrap: 100%) ENSMMUP00000005627 (score: 0.142)	From Inparanoid:20070104
Mus musculus	MGI:1351636 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000014588 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000050774 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YER086W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC16773c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000137987 (score: 1.000; bootstrap: 84%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00031719001 (score: 1.000; bootstrap: 78%)	From Inparanoid:20070104
Shigella flexneri	ILVA	From SHIGELLACYC
E. coli O157	ILVA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00291 Pyridoxal-phosphate dependent enzyme
Superfamily (EcoliWiki Page)	SUPERFAMILY:53686
Panther (EcoliWiki Page)	PTHR10314:SF30
Superfamily (EcoliWiki Page)	SUPERFAMILY:55021
Pfam (EcoliWiki Page)	PF00585 C-terminal regulatory domain of Threonine dehydratase
Pfam (EcoliWiki Page)	PF00585 C-terminal regulatory domain of Threonine dehydratase
EcoCyc	EcoCyc:EG10493	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10493	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000486	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0488	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012321	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

ilvA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ilvA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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