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Gene |

Product(s) |

Expression|

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Standard Name	iap
Gene Synonym(s)	ECK2748, b2753, JW2723^[1], JW2723
Product Desc.	alkaline phosphatase isozyme conversion protein^[2]^[3] Alkaline phosphatase isozyme conversion aminopeptidase; generates alkaline phosphatase isozyme 3 subunit lacking the N-terminal Arg^[4]
Product Synonyms(s)	aminopeptidase in alkaline phosphatase isozyme conversion^[1], B2753^[2]^[1], Iap^[2]^[1] , ECK2748, JW2723, b2753
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): iap^[2]
Regulation/Activity
Quick Links
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Notes

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First 24 aa are predicted to be a type I signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	iap
Mnemonic	Isozymic alkaline phosphatase
Synonyms	ECK2748, b2753, JW2723^[1], JW2723
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	61.96 minutes	MG1655: 2874603..2875640 <gbrowseImage> name=NC_000913:2874603..2875640 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2771442..2772479 <gbrowseImage> name=NC_012967:2771442..2772479 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2760415..2761452 <gbrowseImage> name=NC_012759:2760415..2761452 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2875237..2876274 <gbrowseImage> name=NC_007779:2875237..2876274 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2967145..2968182 <gbrowseImage> name=NC_010473:2967145..2968182 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δiap (Keio:JW2723)	deletion	deletion	PMID:16738554	Shigen CGSC10142^[5]
Δiap-755::kan			PMID:16738554	CGSC:101514
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2723	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTTTCCGCATTGCGCCACCG Primer 2:CCAGACGCCTTCGCCAACTCCTT
1B5	Kohara Phage	Genobase	PMID:3038334
6C8	Kohara Phage	Genobase	PMID:3038334
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 10% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
cysI95::Tn10	Linked marker	CAG12173 = CGSC7425^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:cysC95::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10488	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10488	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000481	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947215	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0483	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009033	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Iap
Synonyms	aminopeptidase in alkaline phosphatase isozyme conversion^[1], B2753^[2]^[1], Iap^[2]^[1] , ECK2748, JW2723, b2753
Product description	alkaline phosphatase isozyme conversion protein^[2]^[3] Alkaline phosphatase isozyme conversion aminopeptidase; generates alkaline phosphatase isozyme 3 subunit lacking the N-terminal Arg^[4]
EC number (for enzymes)	3.4.11.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004177	aminopeptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0031	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007484	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008233	peptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007484	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008233	peptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0645	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source	Notes
Inner membrane	PMID:3316184	EchoLocation:iap	Membrane associated
periplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFSALRHRTA ALALGVCFIL PVHASSPKPG DFANTQARHI ATFFPGRMTG TPAEMLSADY IRQQFQQMGY RSDIRTFNSR YIYTARDNRK SWHNVTGSTV IAAHEGKAPQ QIIIMAHLDT YAPLSDADAD ANLGGLTLQG MDDNAAGLGV MLELAERLKN TPTEYGIRFV ATSGEEEGKL GAENLLKRMS DTEKKNTLLV INLDNLIVGD KLYFNSGVKT PEAVRKLTRD RALAIARSHG IAATTNPGLN KNYPKGTGCC NDAEIFDKAG IAVLSVEATN WNLGNKDGYQ QRAKTPAFPA GNSWHDVRLD NHQHIDKALP GRIERRCRDV MRIMLPLVKE LAKAS
Length	345
Mol. Wt	37.921 kDa
pI	9.3 (calculated)
Extinction coefficient	29,910 - 30,410 (calc based on 9 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-24	UniProt Manual:Signal Peptides	UniProt:P10423
Domain	110..320	PF04389 Peptidase family M28		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=iap taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130660	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947215	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009033	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P10423	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10488	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10488	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947215	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000481	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0483	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	99	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	34	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	102	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
iap
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2874583..2874623 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2753 (EcoliWiki Page)	NCBI GEO profiles for iap
microarray	GenExpDB:b2753 (EcoliWiki Page)	Summary of data for iap from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2874267..2874677) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ08; Well:H6^[7]
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Notes

Accessions Related to iap Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10488	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0483	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2753
EcoGene	EcoGene:EG10488	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000481	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009033	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	IAP	From SHIGELLACYC
E. coli O157	IAP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04389 Peptidase family M28
Superfamily (EcoliWiki Page)	SUPERFAMILY:53187
EcoCyc	EcoCyc:EG10488	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10488	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000481	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0483	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009033	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

iap:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to iap Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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