iap:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Iap

Synonyms

aminopeptidase in alkaline phosphatase isozyme conversion[1], B2753[2][1], Iap[2][1] , ECK2748, JW2723, b2753

Product description

alkaline phosphatase isozyme conversion protein[2][3]

Alkaline phosphatase isozyme conversion aminopeptidase; generates alkaline phosphatase isozyme 3 subunit lacking the N-terminal Arg[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0030288

outer membrane-bounded periplasmic space

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004177

aminopeptidase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0031

F

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007484

P

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007484

F

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0645

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Inner membrane

PMID:3316184[5]

EchoLocation:iap

Membrane associated

periplasm


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MFSALRHRTA ALALGVCFIL PVHASSPKPG DFANTQARHI ATFFPGRMTG TPAEMLSADY
IRQQFQQMGY RSDIRTFNSR YIYTARDNRK SWHNVTGSTV IAAHEGKAPQ QIIIMAHLDT
YAPLSDADAD ANLGGLTLQG MDDNAAGLGV MLELAERLKN TPTEYGIRFV ATSGEEEGKL
GAENLLKRMS DTEKKNTLLV INLDNLIVGD KLYFNSGVKT PEAVRKLTRD RALAIARSHG
IAATTNPGLN KNYPKGTGCC NDAEIFDKAG IAVLSVEATN WNLGNKDGYQ QRAKTPAFPA
GNSWHDVRLD NHQHIDKALP GRIERRCRDV MRIMLPLVKE LAKAS
Length

345

Mol. Wt

37.921 kDa

pI

9.3 (calculated)

Extinction coefficient

29,910 - 30,410 (calc based on 9 Y, 3 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-24

UniProt Manual:Signal Peptides

UniProt:P10423

Domain

110..320

PF04389 Peptidase family M28

PMID:19920124[6]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=iap taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130660

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947215

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009033

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P10423

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10488

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10488

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947215

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000481

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0483

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Ishino, Y et al. (1987) Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J. Bacteriol. 169 5429-33 PubMed
  6. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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