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Product(s) |

Expression|

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Standard Name	hycF
Gene Synonym(s)	ECK2715, b2720, JW2690, hevF, hevH^[1]
Product Desc.	formate hydrogenlyase complex iron-sulfur protein^[2]^[3]; Component of hydrogenase 3^[2]^[3]; formate hydrogenlyase complex^[2]^[3] Formate hydrogenlyase complex iron-sulfur protein^[4]
Product Synonyms(s)	formate hydrogenlyase complex iron-sulfur protein^[1], B2720^[2]^[1], HevH^[2]^[1], HevF^[2]^[1], HycF^[2]^[1] , ECK2715, hevF, JW2690, b2720
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hycABCDEFGHI^[2], OP00208
Regulation/Activity
Quick Links
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Notes

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FhlA regulon. HycF is a probable electron carrier for Hyd-3. Hydrogenase 3 is part of the formate hydrogenlyase complex.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hycF
Mnemonic	Hydrogenase 3
Synonyms	ECK2715, b2720, JW2690, hevF, hevH^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	61.26 minutes, 61.26 minutes	MG1655: 2842774..2842232 <gbrowseImage> name=NC_000913:2842232..2842774 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2739612..2739070 <gbrowseImage> name=NC_012967:2739070..2739612 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2728044..2728586 <gbrowseImage> name=NC_012759:2728044..2728586 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2843408..2842866 <gbrowseImage> name=NC_007779:2842866..2843408 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2935316..2934774 <gbrowseImage> name=NC_010473:2934774..2935316 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔhycF (Keio:JW2690)	deletion	deletion	PMID:16738554	Shigen CGSC10119^[5]
hycF::Tn5KAN-I-SceI (FB20901)	Insertion at nt 147 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20901	does not contain pKD46
hycF::Tn5KAN-I-SceI (FB20902)	Insertion at nt 147 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20902	contains pKD46
ΔhycF724::kan			PMID:16738554	CGSC:101468
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2690	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTTACCTTTATCAAAAAAGT Primer 2:CCGATGGCCTCTTTCATATGGCG
1B2	Kohara Phage	Genobase	PMID:3038334
12F2	Kohara Phage	Genobase	PMID:3038334
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 55% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
cysI95::Tn10	Linked marker	CAG12173 = CGSC7425^[5]	est. P1 cotransduction: 15% ^[6] Synonyms:cysC95::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10479	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10479	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000472	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947048	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0474	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008942	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HycF
Synonyms	formate hydrogenlyase complex iron-sulfur protein^[1], B2720^[2]^[1], HevH^[2]^[1], HevF^[2]^[1], HycF^[2]^[1] , ECK2715, hevF, JW2690, b2720
Product description	formate hydrogenlyase complex iron-sulfur protein^[2]^[3]; Component of hydrogenase 3^[2]^[3]; formate hydrogenlyase complex^[2]^[3] Formate hydrogenlyase complex iron-sulfur protein^[4]
EC number (for enzymes)	1.18.99.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009055	electron carrier activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001450	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009055	electron carrier activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017900	F	Seeded from EcoCyc (v14.0)	complete
	GO:0022900	electron transport chain	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0249	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051536	iron-sulfur cluster binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001450	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051536	iron-sulfur cluster binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017900	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051536	iron-sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0411	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051539	4 iron, 4 sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0004	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of hydrogenase 3	could be indirect
Protein	Subunits of formate hydrogenlyase complex	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFTFIKKVIK TGTATSSYPL EPIAVDKNFR GKPEQNPQQC IGCAACVNAC PSNALTVETD LATGELAWEF NLGHCIFCGR CEEVCPTAAI KLSQEYELAV WKKEDFLQQS RFALCNCRVC NRPFAVQKEI DYAIALLKHN GDSRAENHRE SFETCPECKR QKCLVPSDRI ELTRHMKEAI
Length	180
Mol. Wt	20.308 kDa
pI	7.1 (calculated)
Extinction coefficient	15,470 - 17,220 (calc based on 3 Y, 2 W, and 14 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	33..56	PF00037 4Fe-4S binding domain	PMID:19920124
Domain	68..91	PF00037 4Fe-4S binding domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hycF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130627	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947048	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008942	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P16432	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10479	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10479	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947048	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000472	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0474	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	14^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	7^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hycABCDEFGHI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2842754..2842794 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2720 (EcoliWiki Page)	NCBI GEO profiles for hycF
microarray	GenExpDB:b2720 (EcoliWiki Page)	Summary of data for hycF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hycF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10479	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0474	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2720
EcoGene	EcoGene:EG10479	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000472	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008942	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Oryza gramene	P05312 (score: 1.000; bootstrap: 72%) O98692 (score: 1.000; bootstrap: 73%) Q6QY33 (score: 0.941) Q6QXX0 (score: 0.941) Q6ENA3 (score: 0.941) Q6Z1V7 (score: 0.937) P46722 (score: 0.927) Q6L3D6 (score: 0.911) Q6ENP3 (score: 0.911) P12099 (score: 0.911)	From Inparanoid:20070104
E. coli O157	HYCF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00037 4Fe-4S binding domain
Pfam (EcoliWiki Page)	PF00037 4Fe-4S binding domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:54862
EcoCyc	EcoCyc:EG10479	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10479	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000472	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0474	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008942	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hycF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hycF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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