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Standard Name	hslO
Gene Synonym(s)	ECK3388, b3401, JW5692, yrfI^[1], yrfI
Product Desc.	molecular chaperone Hsp33^[2]^[3] Hsp33, oxidative stress-induced heat shock chaperone; C-terminal zinc-binding motif regulates Hsp33 activity^[4]
Product Synonyms(s)	heat shock protein Hsp33^[1], B3401^[2]^[1], YrfI^[2]^[1], HslO^[2]^[1], Hsp33^[2]^[1] , ECK3388, JW5692, yrfI, b3401
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hslO^[2]
Regulation/Activity
Quick Links
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Notes

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Hsp33 is functionaly down regulated by zinc binding, zinc masks the hydrophobic surfaces under reduced conditions, release of zinc during oxidative induced disulfide bond formation results in the unmasking of the hydrophobic surfaces and partial activation of chaperone function. The redox induced secondary structure changes are primarily localized in the C-terminus, leaving the N-terminus which harbors the dimerization interface largely unaltered.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hslO
Mnemonic	heat shock locus Heat shock locus
Synonyms	ECK3388, b3401, JW5692, yrfI^[1], yrfI
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	76.04 minutes	MG1655: 3527796..3528674 <gbrowseImage> name=NC_000913:3527796..3528674 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3458458..3459336 <gbrowseImage> name=NC_012967:3458458..3459336 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3414953..3415831 <gbrowseImage> name=NC_012759:3414953..3415831 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4110642..4109764 <gbrowseImage> name=NC_007779:4109764..4110642 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3625541..3626419 <gbrowseImage> name=NC_010473:3625541..3626419 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔhslO (Keio:JW5692)	deletion	deletion			PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5692	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCGCAACATGACCAATTACA Primer 2:CCATGAACTTGCGGATCTGCCGG
2F6	Kohara Phage	Genobase	PMID:3038334
1C9	Kohara Phage	Genobase	PMID:3038334
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 39% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
glgP721::Tn10	Linked marker	CAG18638 = CGSC7451^[5]	est. P1 cotransduction: 24% ^[6] Synonyms:zhg-3086::Tn10, zhh-21::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7744	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12930	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004287	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947178	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2766	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011096	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HslO
Synonyms	heat shock protein Hsp33^[1], B3401^[2]^[1], YrfI^[2]^[1], HslO^[2]^[1], Hsp33^[2]^[1] , ECK3388, JW5692, yrfI, b3401
Product description	molecular chaperone Hsp33^[2]^[3] Hsp33, oxidative stress-induced heat shock chaperone; C-terminal zinc-binding motif regulates Hsp33 activity^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009408	response to heat	PMID:8349564	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	tufA	PMID:15690043	Experiment(s):EBI-881279, EBI-888653
Protein	arcB	PMID:15690043	Experiment(s):EBI-888653
Protein	nikD	PMID:15690043	Experiment(s):EBI-888653
Protein	rplC	PMID:15690043	Experiment(s):EBI-888653
Protein	rpsB	PMID:15690043	Experiment(s):EBI-888653
Protein	rpsC	PMID:15690043	Experiment(s):EBI-888653
Protein	rpsE	PMID:15690043	Experiment(s):EBI-888653
Protein	rpsG	PMID:15690043	Experiment(s):EBI-888653
Protein	rpsJ	PMID:15690043	Experiment(s):EBI-888653
Protein	rpsL	PMID:15690043	Experiment(s):EBI-888653
Protein	arcB	PMID:19402753	LCMS(ID Probability):99.0
Protein	nikD	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIMPQHDQLH RYLFENFAVR GELVTVSETL QQILENHDYP QPVKNVLAEL LVATSLLTAT LKFDGDITVQ LQGDGPMNLA VINGNNNQQM RGVARVQGEI PENADLKTLV GNGYVVITIT PSEGERYQGV VGLEGDTLAA CLEDYFMRSE QLPTRLFIRT GDVDGKPAAG GMLLQVMPAQ NAQQDDFDHL ATLTETIKTE ELLTLPANEV LWRLYHEEEV TVYDPQDVEF KCTCSRERCA DALKTLPDEE VDSILAEDGE IDMHCDYCGN HYLFNAMDIA EIRNNASPAD PQVH
Length	294
Mol. Wt	32.778 kDa
pI	4.2 (calculated)
Extinction coefficient	18,910 - 19,660 (calc based on 9 Y, 1 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..274	PF01430 Hsp33 protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hslO taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111586	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947178	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011096	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6Y5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7744	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12930	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947178	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004287	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2766	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	9.05E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	81.892+/-0.407	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.05051+/-0.00631	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
Protein	E. coli K-12 MG1655	6405	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	952	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4261	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hslO
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3527776..3527816 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3401 (EcoliWiki Page)	NCBI GEO profiles for hslO
microarray	GenExpDB:b3401 (EcoliWiki Page)	Summary of data for hslO from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hslO Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7744	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2766	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3401
EcoGene	EcoGene:EG12930	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004287	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011096	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000040168 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YRFI	From SHIGELLACYC
E. coli O157	YRFI	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01430 Hsp33 protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:64397
Superfamily (EcoliWiki Page)	SUPERFAMILY:118352
EcoCyc	EcoCyc:G7744	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12930	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004287	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2766	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011096	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hslO:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hslO Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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