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Standard Name	hscC
Gene Synonym(s)	ECK0643, b0650, JW0645, ybeW^[1], ybeW
Product Desc.	Hsc62, Hsp70 family chaperone, binds to RpoD and inhibits transcription^[2]^[3] Hsc62, DnaK-like chaperone; binds to RpoD and inhibits transcription; inhibits growth when overexpressed; Hsc56(DjlC) is DnaJ-like co-chaperone; ATPase activity is stimulated by GrpE, DjlC; mutant grows slow and is hypersensitive to Cd(II) and UV^[4]
Product Synonyms(s)	Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription^[1], YbeW^[2]^[1], HscC^[2]^[1], B0650^[2]^[1], Hsc62^[2]^[1] , ECK0643, JW0645, ybeW, b0650
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hscC^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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dnaK, hscA, hscC and yegD are paralogs.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hscC
Mnemonic	Heat shock cognate
Synonyms	ECK0643, b0650, JW0645, ybeW^[1], ybeW
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	14.68 minutes	MG1655: 682616..680946 <gbrowseImage> name=NC_000913:680946..682616 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 663179..661509 <gbrowseImage> name=NC_012967:661509..663179 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 583706..585376 <gbrowseImage> name=NC_012759:583706..585376 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 683815..682145 <gbrowseImage> name=NC_007779:682145..683815 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 621948..620278 <gbrowseImage> name=NC_010473:620278..621948 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 735208..733538 <gbrowseImage> name=NC_010473:733538..735208 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔhscC (Keio:JW0645)	deletion	deletion	PMID:16738554	Shigen CGSC8741^[5]
ΔhscC783::kan			PMID:16738554	CGSC:101412
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0645	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGATAATGCAGAACTCGCCAT Primer 2:CCTGGGATCTCAATGGCTAAATA
15D7	Kohara Phage	Genobase	PMID:3038334
3A2	Kohara Phage	Genobase	PMID:3038334
crcA280::Tn10	Linked marker	CAG12077 = CGSC7347^[5]	est. P1 cotransduction: 36% ^[6] Synonyms:zbe-280::Tn10
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[5]	est. P1 cotransduction: 49% ^[6] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6357	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13653	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002964	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945218	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3417	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002225	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HscC
Synonyms	Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription^[1], YbeW^[2]^[1], HscC^[2]^[1], B0650^[2]^[1], Hsc62^[2]^[1] , ECK0643, JW0645, ybeW, b0650
Product description	Hsc62, Hsp70 family chaperone, binds to RpoD and inhibits transcription^[2]^[3] Hsc62, DnaK-like chaperone; binds to RpoD and inhibits transcription; inhibits growth when overexpressed; Hsc56(DjlC) is DnaJ-like co-chaperone; ATPase activity is stimulated by GrpE, DjlC; mutant grows slow and is hypersensitive to Cd(II) and UV^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	yffL	PMID:15690043	Experiment(s):EBI-880828
Protein	clpA	PMID:15690043	Experiment(s):EBI-880828
Protein	rplJ	PMID:15690043	Experiment(s):EBI-880828
Protein	rpsB	PMID:15690043	Experiment(s):EBI-880828
Protein	tnaA	PMID:15690043	Experiment(s):EBI-880828
Protein	helD	PMID:16606699	Experiment(s):EBI-1137559
Protein	hslR	PMID:16606699	Experiment(s):EBI-1137559
Protein	narZ	PMID:16606699	Experiment(s):EBI-1137559
Protein	narG	PMID:16606699	Experiment(s):EBI-1137559
Protein	gyrA	PMID:16606699	Experiment(s):EBI-1137559
Protein	greA	PMID:16606699	Experiment(s):EBI-1137559
Protein	topA	PMID:16606699	Experiment(s):EBI-1137559
Protein	rplF	PMID:16606699	Experiment(s):EBI-1137559
Protein	yfdS	PMID:16606699	Experiment(s):EBI-1137559
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1137559
Protein	sdhA	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpsE	PMID:16606699	Experiment(s):EBI-1137559
Protein	nuoG	PMID:16606699	Experiment(s):EBI-1137559
Protein	ydcP	PMID:16606699	Experiment(s):EBI-1137559
Protein	rplJ	PMID:16606699	Experiment(s):EBI-1137559
Protein	pflB	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpsB	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpoB	PMID:16606699	Experiment(s):EBI-1137559
Protein	mukB	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1137559
Protein	ada	PMID:16606699	Experiment(s):EBI-1137559
Protein	rpmE	PMID:16606699	Experiment(s):EBI-1137559
Protein	rplR	PMID:16606699	Experiment(s):EBI-1137559
Protein	yciH	PMID:16606699	Experiment(s):EBI-1137559
Protein	yhjK	PMID:15690043	Experiment(s):EBI-893359
Protein	yffL	PMID:19402753	MALDI(Z-score):17.053160
Protein	yhjK	PMID:19402753	LCMS(ID Probability):99.4
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDNAELAIGI DLGTTNSLIA VWKDGAAQLI PNKFGEYLTP SIISMDENNH ILVGKPAVSR RTSHPDKTAA LFKRAMGSNT NWRLGSDTFN APELSSLVLR SLKEDAEEFL QRPIKDVVIS VPAYFSDEQR KHTRLAAELA GLNAVRLINE PTAAAMAYGL HTQQNTRSLV FDLGGGTFDV TVLEYATPVI EVHASAGDNF LGGEDFTHML VDEVLKRADV ARTTLNESEL AALYACVEAA KCSNQSPLHI RWQYQEETRE CEFYENELED LWLPLLNRLR VPIEQALRDA RLKPSQIDSL VLVGGASQMP LVQRIAVRLF GKLPYQSYDP STIVALGAAI QAACRLRSED IEEVILTDIC PYSLGVEVNR QGVSGIFSPI IERNTTVPVS RVETYSTMHP EQDSITVNVY QGENHKVKNN ILVESFDVPL KKTGAYQSID IRFSYDINGL LEVDVLLEDG SVKSRVINHS PVTLSAQQIE ESRTRLSALK IYPRDMLINR TFKAKLEELW ARALGDEREE IGRVITDFDA ALQSNDMARV DEVRRRASDY LAIEIP
Length	556
Mol. Wt	61.987 kDa
pI	4.9 (calculated)
Extinction coefficient	51,340 - 51,965 (calc based on 16 Y, 5 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	80..551	PF00012 Hsp70 protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hscC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128633	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945218	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002225	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77319	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6357	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13653	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945218	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002964	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3417	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.3+/-0.049	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hscC
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:682596..682636 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0650 (EcoliWiki Page)	NCBI GEO profiles for hscC
microarray	GenExpDB:b0650 (EcoliWiki Page)	Summary of data for hscC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (682526..682765) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:B9^[7]
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Notes

Accessions Related to hscC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6357	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3417	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0650
EcoGene	EcoGene:EG13653	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002964	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002225	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000036736 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	YBEW	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00012 Hsp70 protein
Panther (EcoliWiki Page)	PTHR19375:SF1
Superfamily (EcoliWiki Page)	SUPERFAMILY:53067
Superfamily (EcoliWiki Page)	SUPERFAMILY:53067
Superfamily (EcoliWiki Page)	SUPERFAMILY:100920
EcoCyc	EcoCyc:G6357	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13653	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002964	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3417	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002225	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

hscC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hscC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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