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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	hpt
Gene Synonym(s)	ECK0124, b0125, JW5009, hprt^[1]^[2]
Product Desc.
Product Synonyms(s)	hypoxanthine phosphoribosyltransferase^[1], B0125^[2]^[1], Hpt^[2]^[1] , ECK0124, JW5009, b0125
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hpt^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hpt
Mnemonic	Hypoxanthine-guanine phosphoribosyltransferase
Synonyms	ECK0124, b0125, JW5009, hprt^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	3.05 minutes	MG1655: 141431..141967 <gbrowseImage> name=NC_000913:141431..141967 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 144234..144770 <gbrowseImage> name=NC_012967:144234..144770 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 141430..141966 <gbrowseImage> name=NC_012759:141430..141966 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 141431..141967 <gbrowseImage> name=NC_007779:141431..141967 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 115535..116071 <gbrowseImage> name=NC_010473:115535..116071 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
hpt(del) (Keio:JW5009)	deletion	deletion			PMID:16738554	Shigen
hpt-1						CGSC:7174
hpt in strain TL505M			Resistant to	Sulfanilamide and hypoxanthine resistance	PMID:6392473
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5009	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACATACTGTAGAAGTAAT Primer 2:CCtTCGTCCAGCAGAATCACTTT
4E11	Kohara Phage	Genobase	PMID:3038334
17C11	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[3]	est. P1 cotransduction: 5% ^[4] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[3]	est. P1 cotransduction: 79% ^[4]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG20098	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20098	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946624	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002443	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4143	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000440	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Hpt
Synonyms	hypoxanthine phosphoribosyltransferase^[1], B0125^[2]^[1], Hpt^[2]^[1] , ECK0124, JW5009, b0125
Product description
EC number (for enzymes)	2.4.2.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004422	hypoxanthine phosphoribosyltransferase activity	PMID:765747	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0032263	GMP salvage	PMID:12070315	IDA: Inferred from Direct Assay		P		complete
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0032264	IMP salvage	PMID:12070315	IDA: Inferred from Direct Assay		P		complete
	GO:0004422	hypoxanthine phosphoribosyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005904	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004422	hypoxanthine phosphoribosyltransferase activity	PMID:12070315	IDA: Inferred from Direct Assay		F		complete
	GO:0004422	hypoxanthine phosphoribosyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.4.2.8	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005904	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006166	purine ribonucleoside salvage	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005904	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006166	purine ribonucleoside salvage	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0660	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009116	nucleoside metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000836	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	slyD	PMID:19402753	LCMS(ID Probability):99.6
Protein	dapD	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MVRDMKHTVE VMIPEAEIKA RIAELGRQIT ERYKDSGSDM VLVGLLRGSF MFMADLCREV QVSHEVDFMT ASSYGSGMST TRDVKILKDL DEDIRGKDVL IVEDIIDSGN TLSKVREILS LREPKSLAIC TLLDKPSRRE VNVPVEFIGF SIPDEFVVGY GIDYAQRYRH LPYIGKVILL DE
Length	182
Mol. Wt	20.616 kDa
pI	5.0 (calculated)
Extinction coefficient	8,940 - 9,190 (calc based on 6 Y, 0 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	7..129	PF00156 Phosphoribosyl transferase domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hpt taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111088	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946624	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000440	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9M2	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG20098	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20098	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946624	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002443	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4143	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.04E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	7860	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1120	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	3511	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hpt
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:141411..141451 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0125 (EcoliWiki Page)	NCBI GEO profiles for hpt
microarray	GenExpDB:b0125 (EcoliWiki Page)	Summary of data for hpt from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hpt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG20098	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4143	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0125
EcoGene	EcoGene:EG20098	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002443	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000440	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G71750 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000006872 (score: 1.000; bootstrap: 100%) ENSBTAP00000019547 (score: 0.868) ENSBTAP00000030572 (score: 0.801) ENSBTAP00000028120 (score: 0.730) ENSBTAP00000019950 (score: 0.473)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00038963 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00013690 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000027831 (score: 1.000; bootstrap: 100%) ENSCAFP00000006328 (score: 0.508)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000006233 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-CDNA-040425-2865 (score: 1.000; bootstrap: 100%) ZDB-GENE-040426-1918 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040625-14 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040625-25 (score: 0.695) ZDB-GENE-040625-17 (score: 0.695) ZDB-CDNA-040625-196 (score: 0.563) ZDB-GENE-980526-324 (score: 0.563) ZDB-CDNA-040425-380 (score: 0.560)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0216388 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000009829 (score: 1.000; bootstrap: 100%) ENSGALP00000033179 (score: 0.657) ENSGALP00000030603 (score: 0.570)	From Inparanoid:20070104
Homo sapiens	ENSP00000298556 (score: 1.000; bootstrap: 100%) ENSP00000318602 (score: 0.579)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000010889 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000019043 (score: 1.000; bootstrap: 100%) ENSMODP00000010875 (score: 0.550) ENSMODP00000002497 (score: 0.345)	From Inparanoid:20070104
Mus musculus	MGI:96217 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000004004 (score: 1.000; bootstrap: 100%) ENSPTRP00000041182 (score: 0.239)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000050910 (score: 1.000; bootstrap: 100%) ENSRNOP00000037266 (score: 0.560)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000155659 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000176529 (score: 0.378) NEWSINFRUP00000136250 (score: 0.320) NEWSINFRUP00000136242 (score: 0.314)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00028357001 (score: 1.000; bootstrap: 100%) GSTENP00012473001 (score: 0.504) GSTENP00022748001 (score: 0.343) GSTENP00010877001 (score: 0.287) GSTENP00025492001 (score: 0.199) GSTENP00025491001 (score: 0.108)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000042790 (score: 1.000; bootstrap: 100%) ENSXETP00000012985 (score: 0.366)	From Inparanoid:20070104
Shigella flexneri	HPT	From SHIGELLACYC
E. coli O157	HPT	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00156 Phosphoribosyl transferase domain
Panther (EcoliWiki Page)	PTHR22573:SF9
Superfamily (EcoliWiki Page)	SUPERFAMILY:53271
EcoCyc	EcoCyc:EG20098	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20098	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002443	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4143	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000440	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 CGSC: The Coli Genetics Stock Center
↑ ^4.0 ^4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hpt:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hpt Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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