hpt:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hpt

Mnemonic

Hypoxanthine-guanine phosphoribosyltransferase

Synonyms

ECK0124, b0125, JW5009, hprt[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

3.05 minutes 

MG1655: 141431..141967
<gbrowseImage> name=NC_000913:141431..141967 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 144234..144770
<gbrowseImage> name=NC_012967:144234..144770 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 141430..141966
<gbrowseImage> name=NC_012759:141430..141966 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 141431..141967
<gbrowseImage> name=NC_007779:141431..141967 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 115535..116071
<gbrowseImage> name=NC_010473:115535..116071 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

hpt(del) (Keio:JW5009)

deletion

deletion

PMID:16738554[3]

Shigen

hpt-1

CGSC:7174

hpt in strain TL505M

Resistant to

Sulfanilamide and hypoxanthine resistance

PMID:6392473[4]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5009

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAACATACTGTAGAAGTAAT

Primer 2:CCtTCGTCCAGCAGAATCACTTT

4E11

Kohara Phage

Genobase

PMID:3038334[6]

17C11

Kohara Phage

Genobase

PMID:3038334[6]

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[7]

est. P1 cotransduction: 5% [8]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[7]

est. P1 cotransduction: 79% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG20098

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20098

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946624

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002443

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4143

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000440

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. Bruce, I et al. (1984) Potentiation by purines of the growth-inhibitory effects of sulphonamides on Escherichia coli K12 and the location of the gene which mediates this effect. J. Gen. Microbiol. 130 2489-95 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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