hmp:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

hmp

Gene Synonym(s)

ECK2549, b2552, JW2536, fsrB, hmpA, G679[1][2]

Product Desc.

EG10456[2][3]

Flavohemoglobin; nitric oxide dioxygenase; dihydropteridine reductase; GSNO and nitrite reductase; has alkylhydroperoxide reductase activity, possibly involved in the repair of lipid membrane oxidative damage[4]

Product Synonyms(s)

fused nitric oxide dioxygenase[1], dihydropteridine reductase 2[1], B2552[2][1], Hmp[2][1], FsrB[2][1], HmpA[2][1] , ECK2549, fsrB, hmpA, JW2536, b2552

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hmp[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Upregulated by GSNO in defined medium (aerobic) as part of the NsrR regulon. hmp mutants are defective in macrophage survival and swarming motility (Stevanin, 2007).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hmp

Mnemonic

Hemoglobin-like protein

Synonyms

ECK2549, b2552, JW2536, fsrB, hmpA, G679[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

57.85 minutes 

MG1655: 2683857..2685047
<gbrowseImage> name=NC_000913:2683857..2685047 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2606534..2607724
<gbrowseImage> name=NC_012967:2606534..2607724 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2569662..2570852
<gbrowseImage> name=NC_012759:2569662..2570852 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2684491..2685681
<gbrowseImage> name=NC_007779:2684491..2685681 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2775622..2776812
<gbrowseImage> name=NC_010473:2775622..2776812 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2683857

Edman degradation

PMID:1601132
PMID:2034230
PMID:9298646
PMID:9724711


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δhmp (Keio:JW2536)

deletion

deletion

PMID:16738554

Shigen
CGSC10019[5]

hmpY29E,H

Y29E,H

15 to 35-fold reduction in NO dioxygenase activity

seeded from UniProt:P24232

hmpY29F

Y29F

30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant

seeded from UniProt:P24232

Δhmp-726::kan

PMID:16738554

CGSC:101386


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2536

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCTTGACGCTCAAACCATCGC

Primer 2:CCaAGCACCTTATGCGGGCCAAA

8E12

Kohara Phage

Genobase

PMID:3038334

6H2

Kohara Phage

Genobase

PMID:3038334

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[5]

est. P1 cotransduction: 47% [6]
Synonyms:zff-208::Tn10

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[5]

est. P1 cotransduction: 38% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10456

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10456

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000449

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947018

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0451

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008396

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Hmp

Synonyms

fused nitric oxide dioxygenase[1], dihydropteridine reductase 2[1], B2552[2][1], Hmp[2][1], FsrB[2][1], HmpA[2][1] , ECK2549, fsrB, hmpA, JW2536, b2552

Product description

EG10456[2][3]

Flavohemoglobin; nitric oxide dioxygenase; dihydropteridine reductase; GSNO and nitrite reductase; has alkylhydroperoxide reductase activity, possibly involved in the repair of lipid membrane oxidative damage[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005344

oxygen transporter activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01252

F

Seeded from EcoCyc (v14.0)

complete

GO:0005344

oxygen transporter activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0561

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000971

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012292

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008941

nitric oxide dioxygenase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01252

F

Seeded from EcoCyc (v14.0)

complete

GO:0008941

nitric oxide dioxygenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.14.12.17

F

Seeded from EcoCyc (v14.0)

complete

GO:0009636

response to toxin

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0216

P

Seeded from EcoCyc (v14.0)

complete

GO:0015671

oxygen transport

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01252

P

Seeded from EcoCyc (v14.0)

complete

GO:0015671

oxygen transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012292

P

Seeded from EcoCyc (v14.0)

complete

GO:0015671

oxygen transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0561

P

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001433

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008333

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017927

F

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0019825

oxygen binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012292

F

Seeded from EcoCyc (v14.0)

complete

GO:0020037

heme binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000971

F

Seeded from EcoCyc (v14.0)

complete

GO:0020037

heme binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012292

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001221

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001433

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008333

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017927

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

nadE

PMID:16606699

Experiment(s):EBI-1143229

Protein

groL

PMID:16606699

Experiment(s):EBI-1143229

Protein

kbaZ

PMID:16606699

Experiment(s):EBI-1143229

Protein

glyA

PMID:16606699

Experiment(s):EBI-1143229
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytoplasm

PMID:7875569

EchoLocation:hmpA


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLDAQTIATV KATIPLLVET GPKLTAHFYD RMFTHNPELK EIFNMSNQRN GDQREALFNA
IAAYASNIEN LPALLPAVEK IAQKHTSFQI KPEQYNIVGE HLLATLDEMF SPGQEVLDAW
GKAYGVLANV FINREAEIYN ENASKAGGWE GTRDFRIVAK TPRSALITSF ELEPVDGGAV
AEYRPGQYLG VWLKPEGFPH QEIRQYSLTR KPDGKGYRIA VKREEGGQVS NWLHNHANVG
DVVKLVAPAG DFFMAVADDT PVTLISAGVG QTPMLAMLDT LAKAGHTAQV NWFHAAENGD
VHAFADEVKE LGQSLPRFTA HTWYRQPSEA DRAKGQFDSE GLMDLSKLEG AFSDPTMQFY
LCGPVGFMQF TAKQLVDLGV KQENIHYECF GPHKVL
Length

396

Mol. Wt

43.867 kDa

pI

5.7 (calculated)

Extinction coefficient

50,880 - 51,130 (calc based on 12 Y, 6 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

154..254

PF00970 Oxidoreductase FAD-binding domain

PMID:19920124

Domain

6..103

PF00042 Globin

PMID:19920124

Domain

264..373

PF00175 Oxidoreductase NAD-binding domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hmp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130477

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947018

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008396

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P24232

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10456

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10456

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947018

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000449

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0451

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

984

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

289

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

697

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hmp

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2683837..2683877 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2552 (EcoliWiki Page)

NCBI GEO profiles for hmp

microarray

GenExpDB:b2552 (EcoliWiki Page)

Summary of data for hmp from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to hmp Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10456

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0451

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2552

EcoGene

EcoGene:EG10456

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000449

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008396

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Dictyostelium discoideum

  • DDB0191099 (score: 1.000; bootstrap: 100%)
  • DDB0191088 (score: 0.432)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YGR234W (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC8692c (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Shigella flexneri

HMPA

From SHIGELLACYC

E. coli O157

HMPA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00042 Globin

Pfam (EcoliWiki Page)

PF00970 Oxidoreductase FAD-binding domain

Pfam (EcoliWiki Page)

PF00175 Oxidoreductase NAD-binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:46458

Superfamily (EcoliWiki Page)

SUPERFAMILY:52343

Panther (EcoliWiki Page)

PTHR22924:SF2

Superfamily (EcoliWiki Page)

SUPERFAMILY:63380

EcoCyc

EcoCyc:EG10456

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10456

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000449

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0451

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008396

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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