hisI:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

hisI

Gene Synonym(s)

ECK2021, b2026, JW2008, hisE, hisIE[1], hisIE

Product Desc.

PR-ATP pyrophosphatase/PR-AMP cyclohydrolase, bifunctional[2]

Product Synonyms(s)

fused phosphoribosyl-AMP cyclohydrolase[1], phosphoribosyl-ATP pyrophosphatase[1], B2026[3][1], HisE[3][1], HisI[3][1] , ECK2021, hisE, hisIE, JW2008, b2026

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hisBHAFI[3], hisLGDCBHAFI[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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After four hours of Zn(II) stress, HisJ protein levels decreased (Easton, 2006).[2]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hisI

Mnemonic

Histidine

Synonyms

ECK2021, b2026, JW2008, hisE, hisIE[1], hisIE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

45.15 minutes, 45.15 minutes 

MG1655: 2094638..2095249
<gbrowseImage> name=NC_000913:2094638..2095249 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2026616..2027227
<gbrowseImage> name=NC_012967:2026616..2027227 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1987121..1987732
<gbrowseImage> name=NC_012759:1987121..1987732 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2098751..2099362
<gbrowseImage> name=NC_007779:2098751..2099362 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2185646..2186257
<gbrowseImage> name=NC_010473:2185646..2186257 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhisI (Keio:JW2008)

deletion

deletion

Auxotrophies

Requires histidine for growth

PMID:16738554

Shigen

CGSC9654[4]

hisITT192GE

TT192GE

Auxotrophies

(in strain: F719)

Strain variation; seeded from UniProt:P06989

hisIE196D

E196D

Auxotrophies

(in strain: F719)

Strain variation; seeded from UniProt:P06989

hisI

Auxotrophies

Requires Histidine for growth

Deletion

hisIH164N

H164N

Auxotrophies

(in strain: F719)

Strain variation; seeded from UniProt:P06989

hisIQ5L

Q5L

Auxotrophies

(in strain: F719)

Strain variation; seeded from UniProt:P06989

hisIL46I

L46I

Auxotrophies

(in strain: F719)

Strain variation; seeded from UniProt:P06989

hisIRK199KN

RK199KN

Auxotrophies

(in strain: F719)

Strain variation; seeded from UniProt:P06989

ΔhisI903

deletion

deletion

Auxotrophies

Requires Histidine for growth

CGSC:7886

ΔhisI724::kan

deletion

deletion

Auxotrophies

Requires Histidine for growth

PMID:16738554

CGSC:101374


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2008

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTTAACAGAACAACAACGTCG

Primer 2:CCtTGATGCCGTTTACGCAGGTT

6D9

Kohara Phage

Genobase

PMID:3038334

21H10

Kohara Phage

Genobase

PMID:3038334

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[4]

est. P1 cotransduction: 67% [5]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[4]

est. P1 cotransduction: % [5]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10451

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10451

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000444

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946515

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0446

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006729

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HisI

Synonyms

fused phosphoribosyl-AMP cyclohydrolase[1], phosphoribosyl-ATP pyrophosphatase[1], B2026[3][1], HisE[3][1], HisI[3][1] , ECK2021, hisE, hisIE, JW2008, b2026

Product description

PR-ATP pyrophosphatase/PR-AMP cyclohydrolase, bifunctional[2]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000105

histidine biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01019

P

Seeded from EcoCyc (v14.0)

complete

GO:0000105

histidine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002496

P

Seeded from EcoCyc (v14.0)

complete

GO:0000105

histidine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008179

P

Seeded from EcoCyc (v14.0)

complete

GO:0000105

histidine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0368

P

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004635

phosphoribosyl-AMP cyclohydrolase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01019

F

Seeded from EcoCyc (v14.0)

complete

GO:0004635

phosphoribosyl-AMP cyclohydrolase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002496

F

Seeded from EcoCyc (v14.0)

complete

GO:0004635

phosphoribosyl-AMP cyclohydrolase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.5.4.19

F

Seeded from EcoCyc (v14.0)

complete

GO:0004636

phosphoribosyl-ATP diphosphatase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01019

F

Seeded from EcoCyc (v14.0)

complete

GO:0004636

phosphoribosyl-ATP diphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008179

F

Seeded from EcoCyc (v14.0)

complete

GO:0004636

phosphoribosyl-ATP diphosphatase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.6.1.31

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01019

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsE

PMID:16606699

Experiment(s):EBI-1141661

Protein

yagR

PMID:16606699

Experiment(s):EBI-1141661

Protein

yghW

PMID:16606699

Experiment(s):EBI-1141661

Protein

frsA

PMID:16606699

Experiment(s):EBI-1141661

Protein

dld

PMID:16606699

Experiment(s):EBI-1141661

Protein

fpr

PMID:16606699

Experiment(s):EBI-1141661

Protein

mdoG

PMID:16606699

Experiment(s):EBI-1141661

Protein

yjhG

PMID:16606699

Experiment(s):EBI-1141661

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1141661

Protein

manC

PMID:16606699

Experiment(s):EBI-1141661

Protein

cspI

PMID:16606699

Experiment(s):EBI-1141661

Protein

rpsQ

PMID:16606699

Experiment(s):EBI-1141661

Protein

ymfG

PMID:16606699

Experiment(s):EBI-1141661

Protein

prpE

PMID:16606699

Experiment(s):EBI-1141661

Protein

deoA

PMID:16606699

Experiment(s):EBI-1141661
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLTEQQRREL DWEKTDGLMP VIVQHAVSGE VLMLGYMNPE ALDKTLESGK VTFFSRTKQR
LWTKGETSGN FLNVVSIAPD CDNDTLLVLA NPIGPTCHKG TSSCFGDTAH QWLFLYQLEQ
LLAERKSADP ETSYTAKLYA SGTKRIAQKV GEEGVETALA ATVHDRFELT NEASDLMYHL
LVLLQDQGLD LTTVIENLRK RHQ
Length

203

Mol. Wt

22.755 kDa

pI

5.2 (calculated)

Extinction coefficient

23,950 - 24,325 (calc based on 5 Y, 3 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

33..107

PF01502 Phosphoribosyl-AMP cyclohydrolase

PMID:19920124

Domain

115..202

PF01503 Phosphoribosyl-ATP pyrophosphohydrolase

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hisI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129967

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946515

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006729

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P06989

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10451

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10451

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946515

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000444

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0446

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

0.714+/-0.029

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.081833061

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

2356

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2042

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1564

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hisBHAFI

hisLGDCBHAFI

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2094618..2094658 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2026 (EcoliWiki Page)

NCBI GEO profiles for hisI

microarray

GenExpDB:b2026 (EcoliWiki Page)

Summary of data for hisI from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to hisI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10451

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0446

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2026

EcoGene

EcoGene:EG10451

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000444

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006729

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT1G31860 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q5NBQ1 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC29A32c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000007666 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

HISI

From SHIGELLACYC

E. coli O157

HISI

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Panther (EcoliWiki Page)

PTHR21256:SF3

Superfamily (EcoliWiki Page)

SUPERFAMILY:101386

Superfamily (EcoliWiki Page)

SUPERFAMILY:141734

Pfam (EcoliWiki Page)

PF01502 Phosphoribosyl-AMP cyclohydrolase

Pfam (EcoliWiki Page)

PF01503 Phosphoribosyl-ATP pyrophosphohydrolase

EcoCyc

EcoCyc:EG10451

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10451

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000444

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0446

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006729

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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