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Standard Name	hinT
Gene Synonym(s)	ECK1089, b1103, JW1089, ycfF^[1], ycfF
Product Desc.	purine nucleoside phosphoramidase^[2]^[3] Purine nucleoside phosphoramidase; aminoacyl-adenylate hydrolase; physological role unknown^[4]
Product Synonyms(s)	purine nucleoside phosphoramidase^[1], B1103^[2]^[1], YcfF^[2]^[1], HinT^[2]^[1] , ECK1089, JW1089, ycfF, b1103
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hinT^[2]
Regulation/Activity
Quick Links
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Notes

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HinT hydrolyzes lysyl-AMP, an activity that may be involved in the regulation of translation or the scavanging of aminoacyl-adenylates (Chou, 2006). hinT is essential for growth under high salt conditions (Chou, 2005). HinT is a member of the HIT (histidine triad) family that contains Ap3A and Ap4A hydrolases. HinT is a low abundance protein.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hinT
Mnemonic	HIstidine triad nucleotide-binding
Synonyms	ECK1089, b1103, JW1089, ycfF^[1], ycfF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	25.03 minutes	MG1655: 1161108..1161467 <gbrowseImage> name=NC_000913:1161108..1161467 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1176454..1176813 <gbrowseImage> name=NC_012967:1176454..1176813 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1065033..1065392 <gbrowseImage> name=NC_012759:1065033..1065392 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1163462..1163821 <gbrowseImage> name=NC_007779:1163462..1163821 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1216553..1216912 <gbrowseImage> name=NC_010473:1216553..1216912 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔhinT (Keio:JW1089)	deletion	deletion			PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1089	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAGAAGAAACTATATTCAG Primer 2:CCaAGACCTTTATGCGCCAGCAT
E9G1	Kohara Phage	Genobase	PMID:3038334
15A8	Kohara Phage	Genobase	PMID:3038334
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 82% ^[6]
zcf-117::Tn10	Linked marker	CAG18463 = CGSC7363^[5]	est. P1 cotransduction: 10% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12172	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12172	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002067	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948549	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2090	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003729	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HinT
Synonyms	purine nucleoside phosphoramidase^[1], B1103^[2]^[1], YcfF^[2]^[1], HinT^[2]^[1] , ECK1089, JW1089, ycfF, b1103
Product description	purine nucleoside phosphoramidase^[2]^[3] Purine nucleoside phosphoramidase; aminoacyl-adenylate hydrolase; physological role unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dnaK	PMID:15690043	Experiment(s):EBI-888025
Protein	dnaN	PMID:15690043	Experiment(s):EBI-888025
Protein	tufA	PMID:15690043	Experiment(s):EBI-888025
Protein	ydeP	PMID:15690043	Experiment(s):EBI-893639
Protein	mltD	PMID:15690043	Experiment(s):EBI-893639
Protein	yjhH	PMID:15690043	Experiment(s):EBI-893639
Protein	nadE	PMID:16606699	Experiment(s):EBI-1138875
Protein	yhbJ	PMID:16606699	Experiment(s):EBI-1138875
Protein	nuoI	PMID:16606699	Experiment(s):EBI-1138875
Protein	tpx	PMID:16606699	Experiment(s):EBI-1138875
Protein	accA	PMID:16606699	Experiment(s):EBI-1138875
Protein	atpG	PMID:16606699	Experiment(s):EBI-1138875
Protein	mukB	PMID:16606699	Experiment(s):EBI-1138875
Protein	mltD	PMID:19402753	LCMS(ID Probability):99.6
Protein	dnaN	PMID:19402753	MALDI(Z-score):37.284431
Protein	ydeP	PMID:19402753	LCMS(ID Probability):99.6
Protein	yjhH	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAEETIFSKI IRREIPSDIV YQDDLVTAFR DISPQAPTHI LIIPNILIPT VNDVSAEHEQ ALGRMITVAA KIAEQEGIAE DGYRLIMNTN RHGGQEVYHI HMHLLGGRPL GPMLAHKGL
Length	119
Mol. Wt	13.241 kDa
pI	6.1 (calculated)
Extinction coefficient	4,470 (calc based on 3 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Modification Site	33	phosphorylation site at S33	probability less than 75%	PMID:17938405
Modification Site	38	phosphorylation site at T38	probability less than 75%	PMID:17938405
Domain	12..110	PF01230 HIT domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hinT taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176077	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948549	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003729	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACE7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12172	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12172	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948549	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002067	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2090	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	73.177+/-0.342	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	3872	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2098	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2005	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hinT
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1161088..1161128 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1103 (EcoliWiki Page)	NCBI GEO profiles for hinT
microarray	GenExpDB:b1103 (EcoliWiki Page)	Summary of data for hinT from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hinT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12172	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2090	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1103
EcoGene	EcoGene:EG12172	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002067	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003729	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000012999 (score: 1.000; bootstrap: 100%) ENSANGP00000029056 (score: 0.179)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000009330 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G56490 (score: 1.000; bootstrap: 99%) AT1G31160 (score: 0.391)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000015208 (score: 1.000; bootstrap: 100%) ENSBTAP00000014552 (score: 0.255) ENSBTAP00000009924 (score: 0.250)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00040575 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00009002 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000003255 (score: 1.000; bootstrap: 100%) ENSCAFP00000001042 (score: 0.220)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040927-8 (score: 1.000; bootstrap: 99%) ZDB-CDNA-040926-16 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0216234 (score: 1.000; bootstrap: 100%) DDB0219436 (score: 0.792)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0031459 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA15490-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000000583 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000259667 (score: 1.000; bootstrap: 100%) ENSP00000304229 (score: 0.243) ENSP00000310489 (score: 0.143)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000021458 (score: 1.000; bootstrap: 100%) ENSMMUP00000013065 (score: 0.073)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000008617 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1916167 (score: 1.000; bootstrap: 100%) MGI:1321133 (score: 0.240)	From Inparanoid:20070104
Oryza gramene	P42856 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000035787 (score: 1.000; bootstrap: 100%) ENSPTRP00000029435 (score: 0.243) ENSPTRP00000033923 (score: 0.162)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000021387 (score: 1.000; bootstrap: 100%) ENSRNOP00000000772 (score: 0.229) ENSRNOP00000007437 (score: 0.224)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDL125C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000156464 (score: 1.000; bootstrap: 99%) NEWSINFRUP00000155788 (score: 0.259)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00014347001 (score: 1.000; bootstrap: 99%) GSTENP00028994001 (score: 0.264)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000018156 (score: 1.000; bootstrap: 59%)	From Inparanoid:20070104
Shigella flexneri	YCFF	From SHIGELLACYC
E. coli O157	YCFF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01230 HIT domain
Panther (EcoliWiki Page)	PTHR23089:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:54197
EcoCyc	EcoCyc:EG12172	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12172	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002067	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2090	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003729	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hinT:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hinT Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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