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Gene |

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Expression|

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Standard Name	hemL
Gene Synonym(s)	ECK0153, b0154, JW0150, gsa, popC, gsa?^[1]^[2], gsa?
Product Desc.	glutamate-1-semialdehyde aminotransferase^[2]^[3]; Component of glutamate-1-semialdehyde aminotransferase^[2]^[3] Glutamate-1-semialdehyde aminomutase^[4]
Product Synonyms(s)	glutamate-1-semialdehyde aminotransferase (aminomutase)^[1], B0154^[2]^[1], Gsa^[2]^[1], PopC^[2]^[1], HemL^[2]^[1] , ECK0153, gsa?, JW0150, popC, b0154
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hemL^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Mg(2+) stimulon-PhoPQ regulon, EvgAS-PhoPQ cascade enhanced expression subset.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hemL
Mnemonic	Hemin
Synonyms	ECK0153, b0154, JW0150, gsa, popC, gsa?^[1]^[2], gsa?
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	3.74 minutes	MG1655: 174882..173602 <gbrowseImage> name=NC_000913:173602..174882 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 177724..176444 <gbrowseImage> name=NC_012967:176444..177724 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 173601..174881 <gbrowseImage> name=NC_012759:173601..174881 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 174882..173602 <gbrowseImage> name=NC_007779:173602..174882 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 148986..147706 <gbrowseImage> name=NC_010473:147706..148986 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
hemLK265R		K265R		2% of wild-type activity			seeded from UniProt:P23893
hemL207			Growth Phenotype	Reduced growth rate	PMID:2045363	CGSC:29164 Experimental strain: AB354	Mutants were grown on L agar supplemented with aminolevulinic acid.
hemL206			Growth Phenotype	Reduced growth rate	PMID:2045363	CGSC:26713 Experimental strain: AB354	Mutants were grown on L agar supplemented with aminolevulinic acid.
hemL::Tn10dCam	Idnsertion		mutagenesis rate	Decrease in Stressed Induced Mutagenesis (SIM).	PMID:23224554	Parent Strain: SMR4562 Experimental Strain: SMR11982	The hemL mutation conferred a strong decrease in the SIM with mutant frequency being decreased by over 90 percent. See table S3 for full experimental data.
hemL::Tn10dCam	Insertion		Sensitivity to	UV sensitivity	PMID:23224554	Parent Strain: SMR4562 Experimental Strain: SMR11982	The mutation conferred an increase in UV sensitivity. See tables S7 and S2 for a summary of experimental data.
CAG45114 hemL::Tn10dCam	Insertion		Sigma^E activity	Decrease in Sigma^E activity	PMID:23224554	Parental Strain: CAG45114 Experimental Strain: SMR15319	See table S11 for full experimental data
hemL			Resistant to	Resistance to Kanamycin	PMID:207952	Experimental Strain: AB354
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0150	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTAAGTCTGAAAATCTTTA Primer 2:CCCAACTTCGCAAACACCCGACG
15A7	Kohara Phage	Genobase	PMID:3038334
9H2	Kohara Phage	Genobase	PMID:3038334
4E4	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 39% ^[6]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 5% ^[6] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10432	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10432	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000425	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946892	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0427	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000529	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HemL
Synonyms	glutamate-1-semialdehyde aminotransferase (aminomutase)^[1], B0154^[2]^[1], Gsa^[2]^[1], PopC^[2]^[1], HemL^[2]^[1] , ECK0153, gsa?, JW0150, popC, b0154
Product description	glutamate-1-semialdehyde aminotransferase^[2]^[3]; Component of glutamate-1-semialdehyde aminotransferase^[2]^[3] Glutamate-1-semialdehyde aminomutase^[4]
EC number (for enzymes)	5.4.3.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015421	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00375	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006779	porphyrin biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00375	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006779	porphyrin biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0627	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008483	transaminase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005814	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016853	isomerase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0413	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030170	pyridoxal phosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005814	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030170	pyridoxal phosphate binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015421	F	Seeded from EcoCyc (v14.0)	complete
	GO:0033014	tetrapyrrole biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004639	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042286	glutamate-1-semialdehyde 2,1-aminomutase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00375	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042286	glutamate-1-semialdehyde 2,1-aminomutase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004639	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042286	glutamate-1-semialdehyde 2,1-aminomutase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.4.3.8	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of glutamate-1-semialdehyde aminotransferase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSKSENLYSA ARELIPGGVN SPVRAFTGVG GTPLFIEKAD GAYLYDVDGK AYIDYVGSWG PMVLGHNHPA IRNAVIEAAE RGLSFGAPTE MEVKMAQLVT ELVPTMDMVR MVNSGTEATM SAIRLARGFT GRDKIIKFEG CYHGHADCLL VKAGSGALTL GQPNSPGVPA DFAKYTLTCT YNDLASVRAA FEQYPQEIAC IIVEPVAGNM NCVPPLPEFL PGLRALCDEF GALLIIDEVM TGFRVALAGA QDYYGVVPDL TCLGKIIGGG MPVGAFGGRR DVMDALAPTG PVYQAGTLSG NPIAMAAGFA CLNEVAQPGV HETLDELTTR LAEGLLEAAE EAGIPLVVNH VGGMFGIFFT DAESVTCYQD VMACDVERFK RFFHMMLDEG VYLAPSAFEA GFMSVAHSME DINNTIDAAR RVFAKL
Length	426
Mol. Wt	45.365 kDa
pI	4.6 (calculated)
Extinction coefficient	26,360 - 27,610 (calc based on 14 Y, 1 W, and 10 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	35..361	PF00202 Aminotransferase class-III		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hemL taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128147	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946892	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000529	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P23893	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10432	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10432	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946892	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000425	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0427	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	7.63E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	4631	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1124	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	3236	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hemL
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:174862..174902 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0154 (EcoliWiki Page)	NCBI GEO profiles for hemL
microarray	GenExpDB:b0154 (EcoliWiki Page)	Summary of data for hemL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (174811..175128) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:G8^[7]
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Notes

Accessions Related to hemL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10432	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0427	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0154
EcoGene	EcoGene:EG10432	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000425	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000529	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT3G48730 (score: 1.000; bootstrap: 100%) AT5G63570 (score: 0.826)	From Inparanoid:20070104
Oryza gramene	Q6YZE2 (score: 1.000; bootstrap: 100%) P18492 (score: 0.792)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPCC4171c (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000011066 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	HEML	From SHIGELLACYC
E. coli O157	HEML	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00202 Aminotransferase class-III
Superfamily (EcoliWiki Page)	SUPERFAMILY:53383
Panther (EcoliWiki Page)	PTHR11986:SF5
EcoCyc	EcoCyc:EG10432	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10432	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000425	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0427	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000529	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

hemL:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hemL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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