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Gene |

Product(s) |

Expression|

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Standard Name	gspA
Gene Synonym(s)	ECK3310, b3323, JW3285, yheD^[1], yheD
Product Desc.	general secretory pathway component, cryptic^[2]^[3] Part of H-NS-silenced gsp divergon, type II protein secretion; cloned gsp divergon secretes ChiA, requires gspAB operon^[4]
Product Synonyms(s)	general secretory pathway component, cryptic^[1], B3323^[2]^[1], YheD^[2]^[1], GspA^[2]^[1] , ECK3310, JW3285, yheD, b3323
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pioO^[2], gspA-pioO^[2], gspAB^[3]
Regulation/Activity
Quick Links
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Notes

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GspA is homologous to exeA of A. hydrophila, which is required for protein export, specifically for the export and assembly of secretin (ExeD/GspD) into the outer membrane. GspA has an N-terminal predicted cytoplasmic domain with an ATP/GTP binding motif, a single predicted transmembrane segment (239-261), followed by a predicted periplasmic domain with a predicted C-terminal peptidoglycan-binding motif (407-467; Pfam PG_binding_1). GspA may interact with GspB. A divergon is a regulon comprised of two adjacent divergent operons or genes. Overexpression causes a decrease in the amount of biofilm formed and decreases motility.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gspA
Mnemonic	General secretory pathway
Synonyms	ECK3310, b3323, JW3285, yheD^[1], yheD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	74.4 minutes	MG1655: 3453420..3451951 <gbrowseImage> name=NC_000913:3451951..3453420 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3383303..3381834 <gbrowseImage> name=NC_012967:3381834..3383303 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3339108..3340577 <gbrowseImage> name=NC_012759:3339108..3340577 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4185018..4186487 <gbrowseImage> name=NC_007779:4185018..4186487 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3551165..3549696 <gbrowseImage> name=NC_010473:3549696..3551165 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔgspA (Keio:JW3285)	deletion	deletion			PMID:16738554	Shigen CGSC10458^[5]
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3285	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCTACGAGAAGAGAAGTTAT Primer 2:CCTTTCCCCTTCACTGTTGTTTC
9H3	Kohara Phage	Genobase	PMID:3038334
E1H9	Kohara Phage	Genobase	PMID:3038334
smg-3082::Tn10	Linked marker	CAG12071 = CGSC7444^[5]	est. P1 cotransduction: 42% ^[6] Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7701	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12888	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004246	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947825	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2725	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010867	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GspA
Synonyms	general secretory pathway component, cryptic^[1], B3323^[2]^[1], YheD^[2]^[1], GspA^[2]^[1] , ECK3310, JW3285, yheD, b3323
Product description	general secretory pathway component, cryptic^[2]^[3] Part of H-NS-silenced gsp divergon, type II protein secretion; cloned gsp divergon secretes ChiA, requires gspAB operon^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002477	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	groL		PMID:16606699	Experiment(s):EBI-1145583
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSTRREVILS WLCEKRQTWR LCYLLGEAGS GKTWLAQQLQ KDKHRRVITL SLVVSWQGKA AWIVTDDNAA EQGCRDSAWT RDEMAGQLLH ALHRTDSRCP LIIIENAHLN HRRILDDLQR AISLIPDGQF LLIGRPDRKV ERDFKKQGIE LVSIGRLTEH ELKASILEGQ NIDQPDLLLT ARVLKRIALL CRGDRRKLAL AGETIRLLQQ AEQTSVFTAK QWRMIYRILG DNRPRKMQLA VVMSGTIIAL TCGWLLLSSF TATLPVPAWL IPVTPVVKQD MTKDIAHVVM RDSEALSVLY GVWGYEVPAD SAWCDQAVRA GLACKSGNAS LQTLVDQNLP WIASLKVGDK KLPVVVVRVG EASVDVLVGQ QTWTLTHKWF ESVWTGDYLL LWKMSPEGES TITRDSSEEE ILWLETMLNR ALHISTEPSA EWRPLLVEKI KQFQKSHHLK TDGVVGFSTL VHLWQVAGES AYLYRDEANI SPETTVKGK
Length	489
Mol. Wt	55.298 kDa
pI	8.6 (calculated)
Extinction coefficient	114,930 - 115,930 (calc based on 7 Y, 19 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	407..463	PF01471 Putative peptidoglycan binding domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gspA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131202	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947825	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010867	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P45756	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7701	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12888	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947825	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004246	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2725	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.17E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pioO
gspA-pioO
gspAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3453400..3453440 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3323 (EcoliWiki Page)	NCBI GEO profiles for gspA
microarray	GenExpDB:b3323 (EcoliWiki Page)	Summary of data for gspA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to gspA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7701	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2725	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3323
EcoGene	EcoGene:EG12888	Escherichia coli str. K-12 substr. MG1655
EcoCyc (EcoliWiki Page)	EcoCyc:TU0-14376
RegulonDB	RegulonDB:ECK120004246	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010867	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00338
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01471 Putative peptidoglycan binding domain
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 ^2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

gspA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gspA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

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Families

Notes

Links

References

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