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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	gph
Gene Synonym(s)	ECK3372, b3385, JW3348, yhfE^[1], yhfE
Product Desc.	GPH^[2]^[3] Phosphoglycolate phosphatase; in dam operon; involved in degradation of 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends; HAD10^[4]
Product Synonyms(s)	phosphoglycolate phosphatase^[1], B3385^[2]^[1], YhfE^[2]^[1], Gph^[2]^[1] , ECK3372, JW3348, yhfE, b3385
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): rpe-gph-trpS^[2], dam-rpe-gph-trpS^[2], damX-dam-rpe-gph-trpS^[2], aroKB-damX-dam-rpe-gph-trpS^[2]
Regulation/Activity
Quick Links
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Notes

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HAD superfamily. gph is a non-essential gene. Gph has weak phosphoramidase activity in vitro (Kuznetsova, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gph
Mnemonic	Glycolate phosphatase
Synonyms	ECK3372, b3385, JW3348, yhfE^[1], yhfE
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	75.69 minutes	MG1655: 3512411..3511653 <gbrowseImage> name=NC_000913:3511653..3512411 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3443071..3442313 <gbrowseImage> name=NC_012967:3442313..3443071 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3398810..3399568 <gbrowseImage> name=NC_012759:3398810..3399568 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4126027..4126785 <gbrowseImage> name=NC_007779:4126027..4126785 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3610156..3609398 <gbrowseImage> name=NC_010473:3609398..3610156 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
Δgph (Keio:JW3348)	deletion	deletion	PMID:16738554	Shigen CGSC10498^[5]
gph::Tn5KAN-I-SceI (FB21144)	Insertion at nt 721 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21144	does not contain pKD46
gph::Tn5KAN-I-SceI (FB21145)	Insertion at nt 721 in Minus orientation		PMID:15262929	E. coli Genome Project:FB21145	contains pKD46
Δgph-720::kan			PMID:16738554	CGSC:101244
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3348	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAATAAGTTTGAAGATATTCG Primer 2:CCGTCATTTTTCGATTCCTGATT
4D4	Kohara Phage	Genobase	PMID:3038334
6F9	Kohara Phage	Genobase	PMID:3038334
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 74% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
glgP721::Tn10	Linked marker	CAG18638 = CGSC7451^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zhg-3086::Tn10, zhh-21::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11871	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11871	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001802	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947895	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1817	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011058	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Gph
Synonyms	phosphoglycolate phosphatase^[1], B3385^[2]^[1], YhfE^[2]^[1], Gph^[2]^[1] , ECK3372, JW3348, yhfE, b3385
Product description	GPH^[2]^[3] Phosphoglycolate phosphatase; in dam operon; involved in degradation of 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends; HAD10^[4]
EC number (for enzymes)	3.1.3.18^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0016052	carbohydrate catabolic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00495	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0119	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008967	phosphoglycolate phosphatase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00495	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008967	phosphoglycolate phosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006346	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008967	phosphoglycolate phosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006439	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008967	phosphoglycolate phosphatase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.18	F	Seeded from EcoCyc (v14.0)	complete
	GO:0031404	chloride ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0868	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	nagA	PMID:16606699	Experiment(s):EBI-1145733
Protein	caiD	PMID:16606699	Experiment(s):EBI-1145733
Protein	otsB	PMID:16606699	Experiment(s):EBI-1145733
Protein	gapA	PMID:16606699	Experiment(s):EBI-1145733
Protein	nadE	PMID:16606699	Experiment(s):EBI-1145733
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNKFEDIRGV AFDLDGTLVD SAPGLAAAVD MALYALELPV AGEERVITWI GNGADVLMER ALTWARQERA TQRKTMGKPP VDDDIPAEEQ VRILRKLFDR YYGEVAEEGT FLFPHVADTL GALQAKGLPL GLVTNKPTPF VAPLLEALDI AKYFSVVIGG DDVQNKKPHP DPLLLVAERM GIAPQQMLFV GDSRNDIQAA KAAGCPSVGL TYGYNYGEAI DLSQPDVIYQ SINDLLPALG LPHSENQESK ND
Length	252
Mol. Wt	27.389 kDa
pI	4.4 (calculated)
Extinction coefficient	22,920 - 23,045 (calc based on 8 Y, 2 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	7..204	PF00702 haloacid dehalogenase-like hydrolase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gph taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131263	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947895	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011058	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P32662	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11871	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11871	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947895	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001802	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1817	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1316	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	432	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	762	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
rpe-gph-trpS
dam-rpe-gph-trpS
damX-dam-rpe-gph-trpS
aroKB-damX-dam-rpe-gph-trpS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3512391..3512431 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3385 (EcoliWiki Page)	NCBI GEO profiles for gph
microarray	GenExpDB:b3385 (EcoliWiki Page)	Summary of data for gph from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to gph Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11871	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1817	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3385
EcoGene	EcoGene:EG11871	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001802	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011058	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000026891 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT2G33255 (score: 1.000; bootstrap: 52%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000040209 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	GPH	From SHIGELLACYC
E. coli O157	GPH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00702 haloacid dehalogenase-like hydrolase
Superfamily (EcoliWiki Page)	SUPERFAMILY:56784
EcoCyc	EcoCyc:EG11871	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11871	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001802	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1817	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011058	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

gph:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gph Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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